Water is extremely important for sustainable development, enabling socio-economic growth, ecological stability, and human survival. Hence, the provision of clean water holds utmost significance. The study aimed to evaluate the quality of tube well water in specific residential areas of Khulna for drinking purposes, comparing it with the standards of World Health Organization (WHO) and Bangladesh (BD) (Environmental Conservation Rules 1997 (ECR, 1997)). Twenty random samples of tube well water were collected from selected residential areas, sourced from deep tube wells with depths ranging from 700 to 1500 ft. The samples were tasted with about 20 parameters, including physical: color, taste, odor, total dissolved solids (TDS) and total suspended solids (TSS); chemical: acidity/alkalinity (pH), arsenic (As), electrical conductivity (EC), dissolved oxygen (DO), bicarbonate (HCO3–), calcium (Ca2+), magnesium (Mg2+), sodium (Na+), chloride (Cl–), iron (Fe), phosphate (PO43–), sulfate (SO42–), potassium (K+); and bacteriological parameters: total coliforms and E. coli bacteria. Most of the samples were found to have higher TDS, TSS, Fe, K+, and Na+ concentrations compared to the WHO and BD Standards. 90 to 95% of samples exhibited acceptable levels of EC, pH, DO, Mg2+, Ca2+, Cl–, and SO43–. The salinity levels in most of the samples were excessive to be used for drinking, and the levels were especially very high in samples S-01 and S-04. The arsenic levels were found to be higher than acceptable limit in S-01 and S-04 as well. E. coli and other bacteria in a few samples were detected.