Impact of routine cell block preparation on results of head and neck fine needle aspirates

Carter, Michael D.; Moore, David P.; MacIntosh, Rebecca F.; Bullock, Martin

doi:10.1002/dc.23597

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…This to our knowledge is one of the few studies that has incorporated the use of cell blocks in the diagnosis of a spectrum of head and neck pathology cases . Contrary to the findings of Carter et al evaluation of the sensitivity of cell block technique (95.56%) in our present study highlights the need for adopting cell block technique for the diagnosis of various head and neck pathologies. Our findings were in agreement with that of Anderson CE et al who also advocate the use of cell blocks for adequate diagnosis of the FNA fluid.…”

Section: Discussioncontrasting

confidence: 95%

Assessment of cell block technique in head and neck pathology diagnoses: A preliminary study

Desai

Angadi

Kale

et al. 2018

Diagnostic Cytopathology

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Objective The head and neck region is a composite site made of multiple tissue components. These tissues when affected by disease or pathology present with an array of changes in the tissue architecture and pattern. It is essential to visualize the cellular details and tissue patterns for accurate diagnosis and treatment planning. Aspiration cytology primarily makes use of the cellular details for diagnosing lesions of the head and neck. Despite the promising results, its use is still limited in certain cases of the head and neck. The reason implicated could be the indiscernible appearance of cells in the absence of tissue integrity. In this regard, cell blocks are known to facilitate the visualization of the cytomorphological as well as the tissue arrangement patterns. Thus, the present study was designed to evaluate the role of cell block cytology in the diagnosis of various lesions of the head and neck. Methods Odontogenic lesions, epithelial carcinomas and connective tissue pathology of the head and neck origin were included in the study (n = 45). Aspiration cytology smears and cell block diagnosis were compared with tissue biopsy diagnosis for determining their sensitivity (%) and diagnostic efficacy. Results Cell blocks showed distinct preservation of the architectural pattern. In case of fluid‐filled lesions, the contents were preserved and correlated with the tissue biopsy results. The results of cell blocks were similar to that of tissue biopsy in majority of the cases (95.56%). Conclusion We recommend using cell blocks as a part of routine laboratory practice for all head‐neck cases.

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Section: Discussioncontrasting

confidence: 95%

Assessment of cell block technique in head and neck pathology diagnoses: A preliminary study

Desai

Angadi

Kale

et al. 2018

Diagnostic Cytopathology

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“…As reported, the cell block, combined with immunostaining, helps in the diagnosis and individualized treatment of cancer patients [36–38]. After centrifuging the NSCLC cell suspension for 3 min at 2,000×g, the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

Clinical significance and effect of AEG-1 on the proliferation, invasion, and migration of NSCLC: a study based on immunohistochemistry, TCGA, bioinformatics, in vitro and in vivo verification

Zhang

Hou

et al. 2017

Oncotarget

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BackgroundAstrocyte elevated gene-1 (AEG-1) is related to the tumorigenesis and deterioration of different cancers, including non-small cell lung cancer (NSCLC). However, the effect of AEG-1 in NSCLC remains unclear. In this study, we aimed to investigate the clinical significance and effect of AEG-1 on biological function of NSCLC.ResultsAEG-1 was significantly overexpressed in NSCLC tissues and closely correlated to the deterioration of NSCLC based on tissue microarray, TCGA database and meta-analysis. After knock-down of AEG-1, the proliferation, migration and invasion of NSCLC cells were all inhibited, and the tumorigenic and angiogenic ability of NSCLC cells were weakened. Furthermore, the AEG-1 co-expressed genes were significantly related to AMPK signaling pathway based on bioinformatics approaches.Materials and MethodsA tissue microarray, the Cancer Genome Atlas (TCGA) database, as well as a meta-analysis were performed to analyze the relationship between AEG-1 and the clinicopathological parameters of NSCLC. Furthermore, immunocytochemistry, Western blot analysis, scratch assay, colony formation assay, Transwell migration and invasion assay and the chick embryo chorioallantoic membrane (CAM) model were conducted to explore the effect of AEG-1 on NSCLC in vitro and in vivo. Additionally, bioinformatics analyses were carried out to assess the potential pathways and networks of the co-expressed genes of AEG-1.ConclusionsAEG-1 is positively activated in the tumorigenesis and deterioration of NSCLC. We hypothesize that AEG-1 could play an important role in NSCLC via AMPK signaling pathway. Inhibiting the expression of AEG-1 is expected to become a novel method in the therapeutic strategies of NSCLC.

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“…CB combines the advantages of histology and cytology and leads to recognition of patterns viewed in histological slides. 28,29 Anderson et al 30 found that CB in head and neck cytology provided additional diagnostic information in 31%…”

Section: Discussionmentioning

confidence: 99%

“…This technique is simple and can be performed in the remaining sample after cytological smear slide preparation. CB combines the advantages of histology and cytology and leads to recognition of patterns viewed in histological slides 28,29 . Anderson et al 30 found that CB in head and neck cytology provided additional diagnostic information in 31% of cases and was essential for making the correct diagnosis in 12% of cases.…”

Section: Discussionmentioning

confidence: 99%

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

et al. 2020

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Introduction: The aim of this study was to perform an audit of oral and maxillofacial specimens submitted for cytological diagnosis to verify the importance of this complementary examination. Methods: A retrospective analysis of our institutional cytopathology database was performed over an 18-year period. Clinical information and cytological data were collected. Associations between independent variables and outcomes were assessed using the Pearson χ 2 test or Fisher's test, with a 5% significance level. When available, the histological diagnosis was compared with cytological diagnosis to identify the percentage of agreement and the specificity, sensitivity and accuracy of cytology in identifying malignant neoplasms. Results: A total of 1082 cases were identified, which included 65 different cytological diagnoses. Exfoliative cytology (EC) was performed in 312 cases (29.1%) and fine needle aspiration cytology (FNAC) in 770 cases (70.9%). EC was mainly employed to diagnose oral infectious diseases (P < 0.001) and FNAC to diagnose neoplasms, cystic, reactive and miscellaneous lesions (P < 0.001). Cell-block was performed in 555 FNAC cases (51.3%). Panoptic, Papanicolaou and haematoxylin-eosin staining were performed in FNAC and periodic acid-Schiff in EC (P < 0.001). In 211 cases (19.5%), the histological diagnosis was available and the percentage agreement with the cytological diagnosis was 41.2%. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy to identify malignant neoplasms were 84.6%, 100%, 100%, 77.8% and 90.0%, respectively. Conclusions: EC was mainly performed for diagnosis of infectious diseases and FNAC for diagnosis of salivary gland tumours, odontogenic lesions, reactive lesions and cervical metastasis. K E Y W O R D S cytopathology, exfoliative cytology, fine needle aspiration cytology, head and neck, oral cavity PÉREZ-DE-OLIVEIRA Et AL. 1 | INTRODUC TI ON Oral diseases can share many clinical aspects, and complementary tests may be needed to achieve a diagnosis. Cytology represents a diagnostic tool based on a microscopic evaluation of the cell samples collected from mucosal surface via exfoliative cytology (EC) or internal sites via fine needle aspiration cytology (FNAC). 1 This complementary examination is widely used in many clinical routines, such as investigation of masses in lymph nodes, liver, thyroid and breast, as well as screening of precursor lesions of cervical carcinoma and follow-up of patients with lung carcinoma. 2,3 In oral diagnosis, the use of cytology examination remains controversial. Previous reports demonstrated that cytopathology can be a useful tool in head and neck lesions. 3,4 Most reports have focused on the thyroid, 5 major salivary gland 6 and cervical lymph node masses, 7 in which, when well indicated, performed and interpreted, cytopathology can achieve a high accuracy to allow for correct therapeutic management. Single reports in the literature suggest that cytopathology applications can go beyond the above-mentioned scenario...

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Impact of routine cell block preparation on results of head and neck fine needle aspirates

Cited by 8 publications

References 15 publications

Assessment of cell block technique in head and neck pathology diagnoses: A preliminary study

Assessment of cell block technique in head and neck pathology diagnoses: A preliminary study

Clinical significance and effect of AEG-1 on the proliferation, invasion, and migration of NSCLC: a study based on immunohistochemistry, TCGA, bioinformatics, in vitro and in vivo verification

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

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