Impact of Rosemary Leave extracts on Triacylglycerol levels in Human Serum Albumin

Abstract

In order to estimate the best affinity of rosemary extract towards the removal of triacylglycerol (TAG) from a real human serum albumin sample HSA in vitro, Rosemary leaves were obtained from Rosmarinus officinalis L plant, and rosemary extracts were prepared with four well selected ethanol-water concentrations combinations and were screened accordingly. The relative affinities of the rosemary (RM) extracts using the selected extraction solvents were evaluated spectrophotometrically and analyzed by applying a well-defined standard equation for each extract. Our results of the current study suggest that the extracts of RM plant could be considered as effective agents to remove TAG from human serum albumin in vitro. The relative affinity is dependent on the concentration of ethanol -water ratio used in the extraction procedure. The relative affinities of plant extracts in this study towards the removal of TAG showed different extends for TAG removal and were varied from 3.96 to 11.16 dependent on ethanol -water ratio. The studied extracts interacted with human serum albumin (HSA) with different binding affinities, which were directly related to their relative affinities to remove TAG from the human serum albumin sample. The present study clearly shows that the highest relative affinity was observed in 50% ethanol -water extracting solution.