Impact of reactive oxygen species on keratinocyte signaling pathways

Bito, Toshinori; Nishigori, Chikako

doi:10.1016/j.jdermsci.2012.06.006

Cited by 80 publications

(54 citation statements)

References 56 publications

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“…ROS can promote tumor initiation by damaging DNA that will lead to mutations and by augmenting signaling pathways that promote cell growth and proliferation [49]. NF-țB, AP-1, and MAPK induction, or STAT3 tyrosine phosphorylation are all triggered by oxidative stress [50], therefore control of ROS levels appears a primary mechanism where Pter could be particularly effective. In this work we focused on Nrf2 signaling as a possible mechanism because the Keap1-Nrf2-ARE pathway is known to control the oxidative stress response [51].…”

Section: Discussionmentioning

confidence: 99%

Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis

Sirerol

Feddi

Mena

et al. 2015

Free Radical Biology and Medicine

107

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Section: Discussionmentioning

confidence: 99%

Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis

Sirerol

Feddi

Mena

et al. 2015

Free Radical Biology and Medicine

107

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“…It has been generally accepted that the generation of ROS by exogenous and endogenous stimuli plays an important role in the homeostasis of skin (49,50). Specifically, the long-term effect of oxidative stress induced by exogenous stimuli such as UV is implicated in skin aging.…”

Section: Discussionmentioning

confidence: 99%

Testosterone Stimulates Duox1 Activity through GPRC6A in Skin Keratinocytes

Choi

Kim

et al. 2014

Journal of Biological Chemistry

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Background:The molecular mechanisms underlying the non-genomic activities of testosterone in keratinocytes are unknown. Results: Testosterone stimulates Duox1 activity through GPRC6A leading to cell death in skin keratinocyte. Conclusion: These results support an understanding of the molecular mechanism of testosterone-dependent apoptosis through Duox1-induced H 2 O 2 generation. Significance: These results provide a novel signaling cascade of testosterone-mediated redox regulation in keratinocytes.

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“…An early response of keratinocytes, which constitute the major epidermal cells of the skin, involves a non-immunological reaction that is characterized by cell damage induced by reactive oxygen species (ROS). ROS can promote the release of pro-inflammatory cytokines from keratinocytes, which in turn activate an immunological response that leads to the progress of contact dermatitis [1,2]. …”

Section: Introductionmentioning

confidence: 99%

A Sensitive Sensor Cell Line for the Detection of Oxidative Stress Responses in Cultured Human Keratinocytes

Hofmann

Priem

Bartzsch

et al. 2014

Sensors

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In the progress of allergic and irritant contact dermatitis, chemicals that cause the generation of reactive oxygen species trigger a heat shock response in keratinocytes. In this study, an optical sensor cell line based on cultured human keratinocytes (HaCaT cells) expressing green fluorescent protein (GFP) under the control of the stress-inducible HSP70B' promoter were constructed. Exposure of HaCaT sensor cells to 25 μM cadmium, a model substance for oxidative stress induction, provoked a 1.7-fold increase in total glutathione and a ∼300-fold induction of transcript level of the gene coding for heat shock protein HSP70B'. An extract of Arnica montana flowers resulted in a strong induction of the HSP70B' gene and a pronounced decrease of total glutathione in keratinocytes. The HSP70B' promoter-based sensor cells conveniently detected cadmium-induced stress using GFP fluorescence as read-out with a limit of detection of 6 μM cadmium. In addition the sensor cells responded to exposure of cells to A. montana extract with induction of GFP fluorescence. Thus, the HaCaT sensor cells provide a means for the automated detection of the compromised redox status of keratinocytes as an early indicator of the development of human skin disorders and could be applied for the prediction of skin irritation in more complex in vitro 3D human skin models and in the development of micro-total analysis systems (μTAS) that may be utilized in dermatology, toxicology, pharmacology and drug screenings.

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Impact of reactive oxygen species on keratinocyte signaling pathways

Cited by 80 publications

References 56 publications

Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis

Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis

Testosterone Stimulates Duox1 Activity through GPRC6A in Skin Keratinocytes

A Sensitive Sensor Cell Line for the Detection of Oxidative Stress Responses in Cultured Human Keratinocytes

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