Impact of prenatal stress on 1H NMR-based metabolic profiling of rat amniotic fluid

Sérrière, Sophie; Barantin, Laurent; François, Sabine; Tranquart, F.; Nadal-Desbarats, Lydie

doi:10.1007/s10334-011-0260-0

Cited by 7 publications

(2 citation statements)

References 54 publications

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“…A decrease in maternal circulating glucose concentrations likely results in decreased fetal uptake of glucose, possibly having long-lasting effects on postnatal responses (Battaglia and Meschia, 1978). Indeed, a study in rats reported a decrease in glucose in the amniotic fluid 2 h following LPS administration on gestational d 19 (Serriere et al, 2011). As stated by Battaglia and Meschia (1978), hypoglycemia in the dam results in hypoglycemia in the fetus, altering the glucose requirements of the fetus.…”

Section: Discussionmentioning

confidence: 99%

Exposure to lipopolysaccharide in utero alters the postnatal metabolic response in heifers1,2,3

Sanchez

Carroll

Arthingon

et al. 2017

Journal of Animal Science

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This study was designed to determine the effect of prenatal lipopolysaccharide (LPS) exposure on the postnatal metabolic response to an LPS challenge in beef heifers. Pregnant, crossbred cows ( = 50) were assigned to a prenatal immune stimulation (PIS; = 25; administered 0.1 µg/kg BW LPS subcutaneously 233 ± 15d of gestation) or saline treatment group (Control; = 25). Birth and weaning BW of calves were collected. There was not (> 0.05) a treatment × gender interaction for birth weight or 205-d adjusted weaning BW. Treatment did not affect (> 0.05) birth BW, but steers and heifers of PIS cows had greater ( < 0.02) 205-d adjusted weaning BW than offspring from Control cows. From the 2 prenatal treatment groups, heifer calves ( = 12 PIS, 11 Control) were identified at weaning (238 ± 15 d of age) to subsequently receive an LPS challenge. On d 0, heifers were fitted with indwelling jugular catheters and were moved into individual pens. On d 1, heifers (fed at 0600 h) were challenged i.v. with LPS (0.5 µg/kg BW) at 0 h (1000 h). Blood samples were collected at 30-min intervals from -2 to 8 h and again at 24 h relative to the LPS challenge. There was a treatment × time interaction ( < 0.01) for cortisol; PIS heifers had greater cortisol from 4 to 6.5 h post-LPS challenge ( < 0.001). There was a treatment × time interaction ( = 0.04) for serum glucose such that glucose was greater ( = 0.01) in PIS than Control heifers at 0.5 h, but was greater in Control than PIS heifers at 2, 4.5, and 7 h post-LPS challenge. This resulted in overall time ( < 0.01) and treatment ( < 0.01) effects such that Control heifers had greater glucose concentrations than PIS heifers. There was a tendency ( = 0.10) for a treatment × time interaction for serum NEFA, such that NEFA was greater in Control than PIS heifers at -2, -1.5, and 7 h relative to the LPS challenge ( ≤ 0.02). Also, there were time ( < 0.01) and treatment effects ( < 0.01) for NEFA with Control heifers having greater NEFA than PIS heifers. Serum BUN was affected by a treatment × time interaction ( < 0.01). Concentrations of BUN were greater in PIS heifers from -1.5 to -1 h, 1 to 2 h, at 4 h, and from 5 to 24 h relative to the LPS challenge. These results demonstrate postnatal growth and the metabolic responses of weaned beef calves can be significantly altered with a single exposure to LPS in utero.

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Section: Discussionmentioning

confidence: 99%

Exposure to lipopolysaccharide in utero alters the postnatal metabolic response in heifers1,2,3

Sanchez

Carroll

Arthingon

et al. 2017

Journal of Animal Science

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“…The AF metabolome has been frequently studied using NMR in clinical and preclinical studies, , but for the past few years, the use of LC–MS is quickly increasing . Several studies already report the use of different solvents such as MeOH or ACN to deproteinize and extract metabolites ,, but without a rationale for their choice.…”

Section: Resultsmentioning

confidence: 99%

Maternal Rat Metabolomics: Amniotic Fluid and Placental Metabolic Profiling Workflows

et al. 2021

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Studying the metabolome of specific gestational compartments is of growing interest in the context of fetus developmental disorders. However, the metabolomes of the placenta and amniotic fluid (AF) are poorly characterized. Therefore, we present the validation of a fingerprinting methodology. Using pregnant rats, we performed exhaustive and robust extractions of metabolites in the AF and lipids and more polar metabolites in the placenta. For the AF, we compared the extraction capabilities of methanol (MeOH), acetonitrile (ACN), and a mixture of both. For the placenta, we compared (i) the extraction capabilities of dichloromethane, methyl t-butyl ether (MTBE), and butanol, along with (ii) the impact of lyophilization of the placental tissue. Analyses were performed on a C18 and hydrophilic interaction liquid chromatography combined with high-resolution mass spectrometry. The efficiency and the robustness of the extractions were compared based on the number of the features or metabolites (for untargeted or targeted approach, respectively), their mean total intensity, and their coefficient of variation (% CV). The extraction capabilities of MeOH and ACN on the AF metabolome were equivalent. Lyophilization also had no significant impact and usefulness on the placental tissue metabolome profiling. Considering the placental lipidome, MTBE extraction was more informative because it allowed extraction of a slightly higher number of lipids, in higher concentration. This proof-of-concept study assessing the metabolomics and lipidomics of the AF and the placenta revealed changes in both metabolisms, at two different stages of rat gestation, and allowed a detailed prenatal metabolic fingerprinting.

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Metabolomics of the amniotic fluid: Is it a feasible approach to evaluate the safety of Chinese medicine during pregnancy?

Shan

Xie

Zhou

et al. 2018

J of Applied Toxicology

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The use of Chinese medicines (CMs) during pregnancy has long been a major public health concern. Although CMs have been shown to be effective in treating infertility and preventing miscarriage, their use has been restricted, mainly because of limited knowledge of their potential toxicity. Accurate toxicology data are urgently required to assess whether these CMs are safe for maternal health and fetal development. Amniotic fluid (AF) contains carbohydrates, lipids and phospholipids, urea and proteins, all of which aid in the growth of the fetus and reflect the mother's health status as well. The changes in metabolomic patterns of AF are related to pathophysiological occurrences during the course of pregnancy. In this review, we provide a summary of the research performed in recent years on metabolomic AF samples, and use our previous study as an example to explore the feasibility of metabolomics of AF to evaluate the safety of CMs during pregnancy. We believe that metabolomics of AF play a far more important role than traditional morphology methods in the safety evaluation of CMs for pregnancy, with a higher sensitivity and correlation.

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Impact of prenatal stress on 1H NMR-based metabolic profiling of rat amniotic fluid

Abstract: Some consistent metabolic changes, observable by (1)H NMR spectroscopy, occurred in amniotic fluid during prenatal stress caused by hyperthermia and LPS-induced inflammation and had an impact on the gestation outcome.

Cited by 7 publications

References 54 publications

Exposure to lipopolysaccharide in utero alters the postnatal metabolic response in heifers1,2,3

Exposure to lipopolysaccharide in utero alters the postnatal metabolic response in heifers1,2,3

Maternal Rat Metabolomics: Amniotic Fluid and Placental Metabolic Profiling Workflows

Metabolomics of the amniotic fluid: Is it a feasible approach to evaluate the safety of Chinese medicine during pregnancy?

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