Impact of pre-incubation time of silk fibroin scaffolds in culture medium on cell proliferation and attachment

Amirikia, Mehdi; Shariatzadeh, Mohammad Ali; Jorsaraei, Seyed Gholam Ali; Mehranjani, Malek Soleimani

doi:10.1016/j.tice.2017.09.002

Cited by 22 publications

(15 citation statements)

References 46 publications

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“…Preincubation resulted in scaffold modifications in the form of reductions in surface roughness and the water contact angle. Moreover, the duration of the preincubation period was proportional to the enhancement of the properties (Amirikia et al, 2017 ). Other natural scaffolds that enhance cellular activities of SCAP are chitosan–based scaffolds.…”

Section: Applications Of Scap In Vivomentioning

confidence: 99%

Stem Cells From the Apical Papilla (SCAP) as a Tool for Endogenous Tissue Regeneration

Nada

Backly

2018

Front. Bioeng. Biotechnol.

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Stem cells extracted from developing tissues possibly exhibit not only unique but also superior traits against their developed counterparts. Indeed, stem cells from the apical papilla (SCAP); a unique group of dental stem cells related to developing roots have been shown to be a promising tool for regenerative endodontic procedures and regeneration in general. Studies have characterized the phenotypic traits as well as other regenerative potentials of these cells. Specific sub-populations have been highlighted as well as their neurogenic and angiogenic properties. Nevertheless, in light of the previously discussed features and potential applications of SCAP, there is still much to understand and a lot of information to unravel. The current review will discuss the role of specific markers for detection of different functional populations of SCAP; including CD146 and STRO-1, as well as their true multilineage differentiation potential. In particular, the role of the secretome in association with paracrine signaling in inflammatory microenvironments is also tackled. Additionally, the role of SCAP both in vitro and in vivo during regenerative approaches and in response to different growth factors and biologic scaffolds is highlighted. Finally, this review will shed light on current knowledge regarding the clinical translational potential of SCAP and elucidate possible areas for future research applications.

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Section: Applications Of Scap In Vivomentioning

confidence: 99%

Stem Cells From the Apical Papilla (SCAP) as a Tool for Endogenous Tissue Regeneration

Nada

Backly

2018

Front. Bioeng. Biotechnol.

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“…For example, increased surface wettability of a silk fibroin scaffold improved the attachment and proliferation of apical papilla stem cells. 34 Decorated micropillars on polymeric substrate surfaces induced osteogenic differentiation of DPSCs. 35 Specifically, the closed side of our scaffold promoted DPSCs osteogenic differentiation in the absence of osteogenic induction medium.…”

Section: Discussionmentioning

confidence: 99%

Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments

Montoya

Orrego

et al. 2019

Cell Proliferation

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Objectives Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic‐co‐glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). Methods The surface morphology of the scaffolds was visualized by scanning electron microscope, and the surface roughness was measured by profilometry. DPSCs were cultured on each side of the scaffolds. Cell morphology, expression of Yes‐associated protein (YAP) and osteogenic/odontogenic differentiation were analysed by immunohistochemistry, real‐time polymerase chain reaction, and Alizarin Red S staining. In addition, cytochalasin D (CytoD), an F‐actin disruptor, was used to examine the effects of F‐actin on intracellular YAP localisation. Verteporfin, a YAP transcriptional inhibitor, was used to explore the effects of YAP signalling on osteogenic/odontogenic differentiation of DPSCs. Results The closed side of our scaffold showed smaller pores and less roughness than the open side. On the closed side, DPSCs exhibited enhanced F‐actin stress fibre alignment, larger spreading area, more elongated appearance, predominant nuclear YAP localization and spontaneous osteogenic differentiation. Inhibition of F‐actin alignments was correlated with nuclear YAP exclusion of DPSCs. Verteporfin restricted YAP localisation to the cytoplasm, down‐regulated expression of early osteogenic/odontogenic markers and inhibited mineralization of DPSCs cultures. Conclusions The surface topographic cues changed F‐actin alignment and morphology of DPSCs, which in turn regulated YAP signalling to control osteogenic/odontogenic differentiation.

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“…Herein, scaffold degradation in culture media without cells was compared to that mediated by osteoclasts, to fully distinguish osteoclasts resorption capacity from Fg‐3D scaffolds cell‐independent degradation. Biomaterials structure modification in culture media is likely to occur due to interactions between serum proteins (Hayman, Pierschbacher, Suzuki, & Ruoslahti, ) and other media constituents with specific motifs, and molecules that compose the biomaterial (Amirikia, Shariatzadeh, Jorsaraei, & Soleimani, ). These changes can interfere with cell adhesion, viability, and proliferation (Wilson, Clegg, Leavesley, & Pearcy, ).…”

Section: Discussionmentioning

confidence: 99%

Osteoclasts degrade fibrinogen scaffolds and induce mesenchymal stem/stromal osteogenic differentiation

Almeida

Bessa-Gonçalves

Vasconcelos

et al. 2019

J Biomedical Materials Res

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Fibrinogen (Fg) is a pro-inflammatory protein with pro-healing properties. Previous work showed that fibrinogen 3D scaffolds (Fg-3D) promote bone regeneration, but the cellular players were not identified. Osteoclasts are bone resorbing cells that promote bone remodeling in close crosstalk with osteoblasts. Herein, the capacity of osteoclasts differentiated on Fg-3D to degrade the scaffolds and promote osteoblast differentiation was evaluated in vitro. Fg-3D scaffolds were prepared by freezedrying and osteoclasts were differentiated from primary human peripheral blood monocytes. Results obtained showed osteoclasts expressing the enzymes cathepsin K and tartrate resistant acid phosphatase colonizing Fg-3D scaffolds. Osteoclasts were able to significantly degrade Fg-3D, reducing the scaffold's area, and increasing D-dimer concentration, a Fg degradation product, in their culture media. Osteoclast conditioned media from the first week of differentiation promoted significantly stronger human primary mesenchymal stem/stromal cell (MSC) osteogenic differentiation, evaluated by alkaline phosphatase activity. Moreover, week 1 osteoclast conditioned media promoted earlier MSC osteogenic differentiation, than chemical osteogenesis inductors. TGF-β1 was found increased in osteoclast conditioned media from week 1, when compared to week 3 of differentiation. Taken together, our results suggest that osteoclasts are able to differentiate and degrade Fg-3D, producing factors like TGF-β1 that promote MSC osteogenic differentiation. K E Y W O R D Sbiomaterial scaffolds, bone regeneration, fibrinogen, mesenchymal stem/stromal cells, osteoclasts

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Impact of pre-incubation time of silk fibroin scaffolds in culture medium on cell proliferation and attachment

Cited by 22 publications

References 46 publications

Stem Cells From the Apical Papilla (SCAP) as a Tool for Endogenous Tissue Regeneration

Stem Cells From the Apical Papilla (SCAP) as a Tool for Endogenous Tissue Regeneration

Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments

Osteoclasts degrade fibrinogen scaffolds and induce mesenchymal stem/stromal osteogenic differentiation

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