Impact of polyphenol-rich green tea extracts on the protection of DOPC monolayer against damage caused by ozone induced lipid oxidation

Rudolphi-Skórska, Elżbieta; Dyba, Barbara; Kreczmer, Barbara; Filek, Maria

doi:10.18388/abp.2018_2612

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…The highest TRO value was demonstrated for EGCG‐combined AgNPs. Rudolphi‐Skórska et al (2018) showed that EGCG is more effective than GA in direct protection of the model cell membrane against oxidative stress. TEM imaging indicated that EGCGAgNPs and EGCGGAAgNPs had the highest cell penetration ability.…”

Section: Discussionmentioning

confidence: 99%

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Barbasz

Czyżowska

Piergies

et al. 2021

J of Applied Toxicology

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Silver nanoparticles (AgNPs) prepared and stabilized by diverse biologically active substances seem to be especially useful in diverse biological and medical applications. The combination of AgNPs with bioactive substances, such as antioxidants, can lead to the development of new systems of desired anticancer properties. In this research, AgNPs were prepared with the use of diverse antioxidant combinations including gallic acid (GA), (−)‐epicatechin‐3‐gallate (EGCG), and caffeine (CAF). The insightful physicochemical characteristic revealed that each type of AgNPs exhibited spherical shape, comparable size distribution and negative surface charge. Surface‐enhanced Raman spectroscopy (SERS) delivered the information about the chemistry of AgNP stabilizing layers, which turned out to be a crucial factor tuning toxicity of AgNPs toward murine B16 melanoma cells (B16‐F0) and human skin melanoma (COLO 679) cells. EGCGAgNPs were the most cytotoxic among all the investigated AgNPs. They strongly reduced the activity of mitochondria, damaged cell membrane integrity, and penetrated inside the cells causing DNA damage. In turn, the toxicity of GAAgNPs strongly manifested via the induction of oxidative stress in the cells. It was found that CAFGAAgNPs exhibited the lowest toxicity toward the melanoma cells, which proved that a proper combination of antioxidants enable to prepare AgNPs of differentiated toxicity. It was established that human skin melanoma cells were significantly more sensitive to AgNPs than the murine melanoma cells.

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Section: Discussionmentioning

confidence: 99%

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Barbasz

Czyżowska

Piergies

et al. 2021

J of Applied Toxicology

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“…Stress conditions were created by introducing ozone into the buffer, where in the water environment ozone undergoes decomposition reactions forming oxidative radicals, among others hydroxyl radicals and superoxide anion radicals [ 35 ]. The obtained mixture of reactive oxygen species corresponds to those naturally occurring in cells, due to the redox status disturbance imbalance [ 36 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Advantageous/Unfavorable Effect of Quercetin on the Membranes of SK-N-SH Neuroblastoma Cells

et al. 2021

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Quercetin is a polyphenolic compound, the effects of which raise scientists’ doubts. The results of many experiments show that it has anticancer, antiinflammatory, and antioxidant properties, while other studies indicate its pro-oxidative and cytotoxic action. This compound can react with reactive oxygen species, and due to its chemical properties, it can be found in the hydrophobic-hydrophilic area of cells. These features of quercetin indicate that its action in cells will be associated with the modification of membranes and its participation in maintaining the redox balance. Therefore, this study distinguishes these two mechanisms and determines whether they are important for cell function. We check: (1) Whether the selected concentrations of quercetin are cytotoxic and destructive for SK-N-SH cell membranes (MTT, LDH, MDA tests) in situations with and without the applied oxidative stress; (2) what is the level of changes in the structural/mechanical properties of the lipid part of the membranes of these cells due to the presence of polyphenol molecules; and (3) whether the antioxidative action of quercetin protects the membrane against its modification. Our results show that changes in the stiffness/elasticity of the lipid part of the membrane constitute the decisive mechanism of action of quercetin, potentially influencing cellular processes whose initial stages are associated with membranes (e.g., reception of signals from the environment, transport).

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“…Green tea contains a many of polyphenols, especially monomeric avonols [75,76]. Since there are very limited studies for investigating protective effects of green tea polyphenols against particulate pollutants [77,78], we examined green tea avonols rich fraction FLGs and its representative compounds, quercetin, kaempferol, and myricetin for damage attenuation by FDP. Among these compounds, myricetin have shown potent positive effects of cell survival under FDP-stimulation dose-dependent manner (Fig.…”

Section: Discussionmentioning

confidence: 99%

Combination of green tea catechins, polysaccharides, and flavonols against urban fine dust particle-induced bronchial damage mediated by modulation of inflammation factors and airway cilia

Kim

Choi

et al. 2020

Preprint

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Background Many studies have reported that environmental pollution has become a serious public health issue. Airborne fine dust particles (FDPs) have been identified as major toxins in air pollution that threaten human respiratory health. Developing strategies for defense against FDPs is one of the primary goals of air pollution research. Results While searching for an anti-FDP reagent, we found that green tea extract (GTE) and green tea fractions rich in flavonol glycosides (FLGs) and crude green tea polysaccharides (CTPs) had protective effects against FDP-stimulated cellular damage in the BEAS-2B airway epithelial cell line. The results demonstrated that GTE, FLGs, and CTPs significantly increased viability and lowered oxidative stress levels in FDP-treated cells. Major catechin and flavonol compounds increased cell survival, and (-)-epigallocatechin gallate (EGCG) and myricetin exerted synergistic effects on cell survival under FDP stimulation. Combined treatment with GTE, FLGs, and CTPs also exerted synergistic protective effects on cells, and the green tea components attenuated FDP-induced elevations in inflammatory gene expression. Moreover, the green tea components increased the proportion of ciliated cells and upregulated ciliogenesis in the airway in FDP-stimulated BEAS-2B cells. Conclusions Our findings provide insights into how natural phytochemicals protect the airway and suggest that green tea could be used to reduce FDP-induced airway damage as an individual therapeutic agent or as an ingredient in pharmaceutical and cosmeceutical products.

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Impact of polyphenol-rich green tea extracts on the protection of DOPC monolayer against damage caused by ozone induced lipid oxidation

Cited by 6 publications

References 20 publications

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Advantageous/Unfavorable Effect of Quercetin on the Membranes of SK-N-SH Neuroblastoma Cells

Combination of green tea catechins, polysaccharides, and flavonols against urban fine dust particle-induced bronchial damage mediated by modulation of inflammation factors and airway cilia

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