Impact of polymer shell on the formation and time evolution of nanoparticle–protein corona

Natte, Kishore; Friedrich, Jörg; Wohlrab, Sebastian; Lutzki, Jana; Klitzing, Regine von; Österle, Werner; Orts-Gil, Guillermo

doi:10.1016/j.colsurfb.2012.11.019

Cited by 52 publications

(52 citation statements)

References 58 publications

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“…Moreover, after measuring the thickness of these images, an increase in diameter of 6±1 nm was computed, which is consistent with the data obtained by DLS, suggesting a BSA monolayer with thickness about 3nm was formed on T-SPIONs and C-SPIONs surface. The formation of protein corona on SPIONs surface will likely change their zeta potential and the isoelectric point (IEP) (Natte et al 2013), thus the change in zeta potential of C-SPIONs and T-SPIONs before and after performing the protocol should also evidence the BSA adsorption on their surface. The zeta potential value of T-SPIONs and C-SPIONs (-54 mV and -42mV, respectively) increased to -24 mV and -22 mV for BSA-T SPIONs and BSA-C-SPIONs respectively (Fig.…”

Section: Characterization Of Bsa Layer On C-spions and T-spionsmentioning

confidence: 99%

Enhanced stability of superparamagnetic iron oxide nanoparticles in biological media using a pH adjusted-BSA adsorption protocol

Laromaine

Roig

2014

J Nanopart Res

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Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biological applications due to their unique properties compared to their bulk counterparts, simplified SPIONs stabilization protocols applicable for a wide spectra of biological media remains a challenging issue. In this work, SPIONs with different surface coatings, tetramethylammonium hydroxide coated SPIONs (T-SPIONs) and citrate coated SPIONs (C-SPIONs), were synthesized by a facile, rapid and cost effective microwave assisted method. CSPIONs show robust stability in biological media of PBS and RPMI, while destabilize in DMEM. T-SPIONs were found to aggregate rapidly and significantly in all tested media. Then, a modified pH adjusted-BSA adsorption protocol and an addition of excess trisodium citrate dihydrate (Na 3 Cit) were used to enhance their stability in the media. The BSA adsorption protocol showed great efficiency in stabilizing the dispersed state of both SPIONs in the tested media, while the addition of excess Na 3 Cit showed limit effect, and it was only applicable for C-SPIONs. The formed BSA layer on SPIONs could be imaged by negative staining TEM, and revealed by Cryo-TEM, FTIR, DLS and the zeta potential measurements. Results indicated that BSA forms a monolayer of a thickness of about 3 ± 1 nm and BSA interacts with C-SPIONs and T-SPIONs through the coating of the SPIONs, rather than by replacing them and interacting directly with the SPIONs surface. This synthetic method and stabilization protocol offer a general methodology to obtain SPIONs with a variety of surfactants, stable in different biological media in few minutes.

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Section: Characterization Of Bsa Layer On C-spions and T-spionsmentioning

confidence: 99%

Enhanced stability of superparamagnetic iron oxide nanoparticles in biological media using a pH adjusted-BSA adsorption protocol

Laromaine

Roig

2014

J Nanopart Res

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“…112 NP-protein interaction offers potential way to design nanoparticulate drug delivery systems in which controlling protein and protein corona can mask NP surface and perform desirable biological functions. 113 The "dysopsonins" such as BSA and Apo J offer a simple way to create and enrich the "stealth effect" for NPs, in which NPs are just mixed and controlled with human plasma or a specific protein component before administration, 69,72 instead of undergoing complicated preparation procedures of NPs. Protein structural modification and approachability of adsorbed proteins should be carefully considered in the tailoring of preformed protein NPs for effective therapeutic activities mediated through binding with relevant cell receptors.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…66,67 Besides, the degree of surface coverage is also an important consideration in design and preparation of NPs to prevent protein adsorption. 68,69 Among all polymers, PEG is the most commonly used polymer for NP surface modification. NPs surrounded by PEG increased blood circulation time by several orders of magnitude and reduced liver accumulation, depending on the coated PEG molecular weight and surface density.…”

Section: Systemic Circulation Time Of Npsmentioning

confidence: 99%

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Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

521

343

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After administration of nanoparticle (NP) into biological fluids, an NP–protein complex is formed, which represents the “true identity” of NP in our body. Hence, protein–NP interaction should be carefully investigated to predict and control the fate of NPs or drug-loaded NPs, including systemic circulation, biodistribution, and bioavailability. In this review, we mainly focus on the formation of protein corona and its potential applications in pharmaceutical sciences such as prediction modeling based on NP-adsorbed proteins, usage of active proteins for modifying NP to achieve toxicity reduction, circulation time enhancement, and targeting effect. Validated correlative models for NP biological responses mainly based on protein corona fingerprints of NPs are more highly accurate than the models solely set up from NP properties. Based on these models, effectiveness as well as the toxicity of NPs can be predicted without in vivo tests, while novel cell receptors could be identified from prominent proteins which play important key roles in the models. The ungoverned protein adsorption onto NPs may have generally negative effects such as rapid clearance from the bloodstream, hindrance of targeting capacity, and induction of toxicity. In contrast, controlling protein adsorption by modifying NPs with diverse functional proteins or tailoring appropriate NPs which favor selective endogenous peptides and proteins will bring promising therapeutic benefits in drug delivery and targeted cancer treatment.

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“…The formation of the corona supports the idea that unmodified NP do not exist in vivo, because as soon as they are administered the adsorption of proteins present in the blood with more affinity for the particle surface will immediately modify them, thus forming a weak layer (soft corona) or a more or less tightly bound layer (hard corona) [334,335]. The binding of different proteins to the NP shell not only influences their surface charge of the NP, but also modifies their total size and can hide functional groups.…”

Section: Fate Of Nanomaterialsmentioning

confidence: 61%

Diagnostic and Therapeutic Uses of Nanomaterials in the Brain

Garbayo¹,

Mendoza²,

Blanco-Prieto

2014

CMC

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Nanomedicine has recently emerged as an exciting tool able to improve the early diagnosis and treatment of a variety of intractable or age-related brain disorders. The most relevant properties of nanomaterials are that they can be engineered in such a way that they can cross the blood brain barrier, with the final aim of targeting specific cells and molecules and to act as vehicles for drugs. Potentially beneficial properties of nanotherapeutics derived from its unique characteristics include improved efficacy, safety, sensitivity and personalization compared to conventional medicines.In this review, recent advances in available nanostructures and nanomaterials for brain applications will be described. Then, the latest nanotechnological applications for the treatment and diagnosis of neurological disorders, mainly brain tumors and neurodegenerative diseases, will be reviewed. Recent investigations of the neurotoxicity of the nanomaterial both in vitro and in vivo will be summarized. Finally, the ongoing challenges that have to be meet if new nanomedical products are to be put on the market will be discussed and some future directions will be outlined.

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Impact of polymer shell on the formation and time evolution of nanoparticle–protein corona

Cited by 52 publications

References 58 publications

Enhanced stability of superparamagnetic iron oxide nanoparticles in biological media using a pH adjusted-BSA adsorption protocol

Enhanced stability of superparamagnetic iron oxide nanoparticles in biological media using a pH adjusted-BSA adsorption protocol

Protein corona: a new approach for nanomedicine design

Diagnostic and Therapeutic Uses of Nanomaterials in the Brain

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