Impact of plant genotype and plant habitat in shaping bacterial pathobiome: a comparative study in olive tree

Abstract

Plant-inhabiting microorganisms interact directly with each other affecting disease progression.However, the role of host plant and plant habitat in shaping pathobiome composition and their implication for host susceptibility/resistance to a particular disease are currently unknown. For the elucidation of these questions, both epiphytic and endophytic bacterial communities, present in asymptomatic and symptomatic twigs from olive cultivars displaying different susceptibilities to olive knot (OK) disease, were investigated using culturing methods. OK disease was the main driver of the bacterial community, causing changes on their diversity, abundance and composition. OK disease effect was most notorious on OK-susceptible cultivar and when considering the endophytic communities. plant habitat (epiphytes vs. endophytes) also contributed to the bacterial community assembling, in particular on symptomatic twigs (knots) of OK-susceptible cultivar. In contrast, host cultivar had little effect on the bacterial community composition, but OK-symptomatic twigs (knots) revealed to be more affected by this driver. Overall, the pathobiome seems to result from an intricate interaction between the pathogen, the resident bacteria, and the plant host. Specific bacterial genera were associated to the presence or absence of OK disease in each cultivar. Their ability to trigger and/or suppress disease should be studied in the future.

Comunidades epifíticas em citros associadas ao huanglongbing em áreas com e sem aplicação de biofertilizante

“…Diferenças na diversidade da comunidade bacteriana em função do genótipo do hospedeiro também foram observadas em ramos de oliveira sintomáticos e assintomáticos em estudos quanto a relação entre a microbiota da filosfera e o patógeno Pseudomonas savastanoi pv. savastanoi (Mina et al, 2020). Isto indica que as interações entre as plantas, os microrganismos benéficos e os patógenos não depende apenas de um fator.…”

Screening the Olive Tree Phyllosphere: Search and Find Potential Antagonists Against Pseudomonas savastanoi pv. savastanoi

“…In this work, the bacterial isolates previously obtained from the phyllosphere (epiphytes and endophytes) of two olive cultivars ( Cobrançosa and Verdeal Transmontana , more tolerant and susceptible to OK disease, respectively) and plant organs (leaves, twigs and knots) ( Mina et al, 2020a , b ) were further studied ( Supplementary Table S1 ). The 60 isolates belonged to 22 different bacterial genera and to 40 species.…”

“…These isolates were originally isolated and identified from symptomless olive tree leaves and twigs, as well as from knots, of cvs. Cobrançosa and Verdeal Transmontana growing in Mirandela (Northeast of Portugal), as referred in Mina et al (2020a , b ). Briefly, for the isolation of epiphytes, bacterial suspensions made from pieces of plant tissues in peptone water were poured over nutrient agar plates.…”

“…Endophytes were isolated from the same plant pieces, by inoculating surface sterilized plant fragments previously dissected into small segments (ca. 4–5 mm) on nutrient agar plates ( Mina et al, 2020a , b ). A total of 60 isolates [stored in 30% (v/v) glycerol at −80°C] were selected for this study, including five isolates from each population (2 plant cultivars × 3 plant organs × 2 microbial habitats; Supplementary Table S1 ).…”

Short review on recent progress in Mn4+‐activated oxide phosphors for indoor plant light‐emitting diodes

“…The blue, red, and FR lights help in plant growth via different mechanisms such as phototropic, photosynthesis, and photomorphogenesis processes. [ 8–15 ] In addition to this, there is shade avoidance syndrome in plants, exhibited by variation in the concentration of phytochrome P R and P FR. [ 8 ] The concentration of these two phytochromes is affected by the variation in the intensity of the R to FR ratio.…”