Impact of phytic acid on nutrient bioaccessibility and antioxidant properties of chickpea genotypes

Abstract

Phytic acid is considered as the major storage pool for phosphorus in the seeds. Present study was experimented on 60 chickpea genotypes for MIPS gene, antioxidant activity, mineral content and phytic acid content. PCR analysis successfully amplified 400 bp fragment of MIPS gene in chickpea. The average phytic acid content observed across all the genotypes was 59.27 mg/100 g. The mineral content assorted for Fe and Zn was 2.41–129.98 mg/100 g and 313.58–1,490.47 mg/100 g, respectively. Significant association of high phytic acid and its mole ratio to zinc was depicted. The relationship between phytic acid content and mineral bioavailability was strongest in Fe (r = 0.448**) followed by Zn (r = 0.959**). This suggest that phytic acid significantly reduce the mineral bioaccessibility. Cluster analysis revealed the distinctness among genotypes on the basis of phytic acid and antioxidant activity and grouped them into seven clusters. Cluster (a) contains low phytic acid genotypes while cluster (b) grouped most of the high phytic acid contenting genotypes. Practical applications Phytic acid chelates micronutrient which prevent their bioavailability. Objectives of plant breeding programs demand enhancement of micronutrient concentration, antioxidant levels with low levels of phytic acid. The present study identified chickpea genotype KWR‐108 with high Fe and Zn contents with average levels of phytic acid. This genotype show prospective food source for commercial applications and can be used as a parent for designing future breeding program.

Moth bean (Vigna aconitifolia (Jacq.) Marechal) seeds: A review on nutritional properties and health benefits

“…These levels observed to be lesser than reported for chickpea (0.75-0.80 g 100 g −1 ), black gram (0.65-0.67 g 100 g −1 ) and pigeon pea (0.01-1.66 g 100 g −1 ), suggesting nutritive value of raw moth bean seeds to comparatively higher extent. Food processing methods like soaking, germination and fermentation reduce phytic acid content in the moth bean seeds [61]. On comparing with other common legumes, moth bean contained significantly lower antinutritional factors.…”

Role of Molecular Markers in Identification and Characterization of Chickpea Variety

“…Chickpea seed contents and chickpea lectins are being characterized substantially at our research laboratory [2,10]. This includes screening of lectins/phytohemagglutinin in more than 100 genotypes [11], purification and characterization [11][12][13], antimicrobial activity [12], fungal resistance [11], MIPS gene [14], anticancer activities [15], apoptosis inducing activity [16], and its various pharmacological attributes [17].…”

Testing Dna Variation in Kabuli Chickpea Seeds Through Issr-PCR

“…Chickpea seed contents and chickpea lectins are being characterized substantially [16,17]. This includes screening of lectins/phytohemagglutinin in more than 100 genotypes [18], purification and characterization [16,17,19], X-ray crystallography [20], microbial resistance [16], antifungal activity [17], MIPS gene [21], anticancer activities [3], apoptosis inducing activity [22], and its various pharmacological significance [4].…”