Impact of Physicochemical Modifications in Casein Promoted by UV-C on the Peptide Profile of Gastric Digestion and the Transepithelial Transport of Peptides

Abstract

Caseins are the main proteins in milk, and their structure and spatial conformation are responsible for their slow digestion rate. The release of bioactive and β-casomorphin peptides from casein digestion may induce allergic responses during consumption. Spectroscopic techniques were used to observe the structural changes in casein conformation induced by Ultraviolet light irradiation (UV-C). Raman spectroscopy results showed more pronounced peaks at 618 and 640 cm −1 for phenylalanine and tyrosine moieties of the photolyzed micellar casein, respectively, suggesting changes in the micelle structure. The decrease in the intensity of Raman signals for tryptophan and tyrosine corroborates to the UV-C-induced modifications of the micelle structure. Particle size distribution showed a decrease in the average micelle size after 15 min of UV-C exposure, while low-temperature, longtime (LTLT) pasteurization led to the formation of large aggregates, as observed by atomic force microscopy. UV-C did not impact the formation or transport of peptides, as observed by using the Caco-2 cell as a model for peptide absorption. However, the absence of the opioid peptide SRYPSY from κ-casein and only 20% of the concentration of opioid peptide RYLGY were noted. This work demonstrated that UV-C can be utilized to induce the physicochemical modification of dairy products, promoting a higher digestion rate and reducing allergenicity.

Exploration of Novel Plasmin Inhibitor from β-Lactoglobulin for Enhancing the Storage Stability of UHT Milk

“…β-Lg was dissolved in simulated milk ultrafiltrate (SMUF). 17 High pressure-resistant borosilicate glass tubes (18 mm × 120 mm) were used for UHT treatment. For the preheating step, 3 mL of β-Lg solution was loaded into the tube and heated in a 75 °C water bath for 5 min.…”

“…β-Lg was dissolved in simulated milk ultrafiltrate (SMUF) . High pressure-resistant borosilicate glass tubes (18 mm × 120 mm) were used for UHT treatment.…”

Fabricating Ultra-Narrow Precision Slit Structures with Periodically Reducing Current Over-Growth Electroforming

“…A micro-scale slit structure is used as the primary component with the function of light filtering, collimation, and diffraction [ 1 , 2 , 3 , 4 ], and the slit width directly affects the sensitivity and resolution in the field of spectrometry [ 5 , 6 , 7 ]. The slits for optical applications are often several micrometers in width and tens of millimeters in length.…”