Impact of Phenylpropanoid Compounds on Heat Stress Tolerance in Carrot Cell Cultures

Commisso, Mauro; Toffali, Ketti; Strazzer, Pamela; Stocchero, Matteo; Ceoldo, Stefania; Baldan, Barbara; Levi, Marisa; Guzzo, Flavia

doi:10.3389/fpls.2016.01439

Cited by 54 publications

(29 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The phenylpropanoid biosynthetic pathway is responsible for the synthesis of phenolic compounds [35]. However, biosynthesis stimulation of these compounds is mainly due to the regulation of many genes encoding the main enzymes of the phenylpropanoid pathway according to environmental conditions [36], such as drought stress [37] and high temperature [38,39]. The effect of genotype on phenolic compounds has been previously shown [40].…”

Section: Discussionmentioning

confidence: 99%

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

et al. 2019

View full text Add to dashboard Cite

The phenol content of vegetable oil and its antioxidant activity are of primary interest for human health. Oilseed species are considered important sources of these compounds with medicinal effects on a large scale. Total phenol content (TPC) and antioxidant activity (AA) of safflower oil were previously studied. Nevertheless, there is no report on genotypic differences and antiaging activity of safflower oil. The aim of this study was to determine the TPC, diphenyl-picrylhydrazyl (DPPH), and antiaging activity on three respective accessions from Syria, France, and Algeria of seed oil of safflower grown under semi-arid conditions during 3 consecutive years (2015, 2016, and 2017). The results showed that phenol content as well as antioxidant and antiaging activity varied according to both genotype and years. In 2017, the mean value of TPC in oil seed was two times higher than in 2015 and 2016. Moreover, accessions presented different TPC values depending on the year. The highest antioxidant activity was observed among accessions in 2017 compared to 2015 and 2016. As expected, a positive correlation was found between TPC and antioxidant activity. The inhibition in the collagenase assay was between 47% and 72.1% compared to the positive control (83.1%), while inhibition in the elastase assay of TPC ranged from 32.2% to 70.3%, with the positive control being 75.8%. These results highlight the interest of safflower oil as a source of phenols with valuable antioxidant and antiaging activity, and uses for cosmetics.

show abstract

Section: Discussionmentioning

confidence: 99%

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Isoprene altered the expression of genes that enhance tolerance to heavy metals, xenobiotics, secondary metabolites, soil acidity and proton toxicity, high light, heat, salt, drought, and oxidative stress. For example, phenylpropanoids are known to enhance tolerance to heat stress as well as biotic stress like herbivory/ wounding (Commisso et al, 2016;Lv et al, 2016). Expression of phenylpropanoid pathway enzymes was higher in gray poplar control lines than in those with RNAi-mediated suppression of isoprene under moderate heat stress (Behnke et al, 2010).…”

Section: Isoprene Executes Its Protective Functions By Altering Exprementioning

confidence: 99%

“…NAD(H) kinase1 remedies oxidative damage under a variety of stresses, including H 2 O 2 and ozone (Berrin et al, 2005;Chai et al, 2006;Hashida et al, 2009). Phenylpropanoids have been shown to protect the actin cytoskeleton in cells against heat damage (Commisso et al, 2016), and the expression of several PAL genes was up-regulated by isoprene. Thus, we see a clear connection between isoprene-mediated alterations in the transcriptome and physiological responses to stress by isoprene-emitting lines.…”

Section: Isoprene Executes Its Protective Functions By Altering Exprementioning

confidence: 99%

Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth

et al. 2019

View full text Add to dashboard Cite

Isoprene synthase converts dimethylallyl diphosphate to isoprene and appears to be necessary and sufficient to allow plants to emit isoprene at significant rates. Isoprene can protect plants from abiotic stress but is not produced naturally by all plants; for example, Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum) do not produce isoprene. It is typically present at very low concentrations, suggesting a role as a signaling molecule; however, its exact physiological role and mechanism of action are not fully understood. We transformed Arabidopsis with a Eucalyptus globulus isoprene synthase. The regulatory mechanisms of photosynthesis and isoprene emission were similar to those of native emitters, indicating that regulation of isoprene emission is not specific to isoprene-emitting species. Leaf chlorophyll and carotenoid contents were enhanced by isoprene, which also had a marked positive effect on hypocotyl, cotyledon, leaf, and inflorescence growth in Arabidopsis. By contrast, leaf and stem growth was reduced in tobacco engineered to emit isoprene. Expression of genes belonging to signaling networks or associated with specific growth regulators (e.g. gibberellic acid that promotes growth and jasmonic acid that promotes defense) and genes that lead to stress tolerance was altered by isoprene emission. Isoprene likely executes its effects on growth and stress tolerance through direct regulation of gene expression. Enhancement of jasmonic acid-mediated defense signaling by isoprene may trigger a growth-defense tradeoff leading to variations in the growth response. Our data support a role for isoprene as a signaling molecule.

show abstract

“…It has been shown that exogenous application of GABA helps coping a variety of plant species with adverse effects of these stress cues [42] either by increasing the antioxidant metabolism [16], the osmotic adjustment [22], maintaining or improving the integrity of cell membrane [43], or by inhibiting the formation of malondialdehyde (MDA) during lipid peroxidation [44]. In carrots, most of the previous studies were focused on managing the damaging effects of abiotic stresses by increasing the level of their tolerance through conventional breeding [45], activation of the molecular network including signal transduction [46], production of stress-specific metabolites [47], expression of genes related to unambiguous stresses [48], development of functional and/or regulatory genes [49,50], and chemical priming [51,52]. Most of these management strategies are either practically complex or beyond the financial affordability of the growers thereby warranting to explore simple and cheaper alternatives with commercial implications.…”

Section: Resultsmentioning

confidence: 99%

Foliar Application of Y-Aminobutyric Acid (GABA) Improves Vegetative Growth, and the Physiological and Antioxidative Potential of Daucus Carota L. under Water Deficit Conditions

Bashir¹,

Hn²,

Shafiq³

et al. 2019

Preprint

View full text Add to dashboard Cite

Scarcity of water is one of the most serious concerns in plant biology with diverse implications at all the levels of molecular, biochemical, and physiological phenomena of plant growth, development, and consequently the productivity. Most of the strategies to induce or enhance drought tolerance in plants are unreasonably expensive and/or time-consuming. Some studies conducted in the recent past have shown that plant growth regulators (PGRs) may induce/improve physiological tolerance in plants to cope with adverse environmental conditions including drought. The present study was aimed at investigating the effects of foliar spray of GABA (0, 1, 2, and 4 mM) applied 20 days following the germination of seeds, on vegetative growth, morphological characteristics, integrity of cell-membrane, and the levels of photosynthetic pigments and enzymatic antioxidants in carrot cvs. Supertaj and Bharat, grown under 100% and 50% field capacity of soil moisture. The treated and untreated (control) carrot plants were harvested and analyzed 2 weeks following the GABA application. The results revealed that foliar application of GABA improved the vegetative growth and significantly increased the levels of free amino acids, plastid pigments, enzymatic antioxidants, and the relative water content in the root crop grown under 50% field capacity of soil moisture, compared to control. Additionally, the GABA application decreased the electrolyte leakage of ions and melondialdehyde (MDA) content in carrot leaves. The carrots harvested from GABA-treated or untreated (control) plants were not significantly different for their protein contents. In conclusion, the incorporation of GABA in the production management of carrots may help plants to mitigate the adverse effects of water deficit stress.

show abstract

Impact of Phenylpropanoid Compounds on Heat Stress Tolerance in Carrot Cell Cultures

Cited by 54 publications

References 70 publications

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth

Foliar Application of Y-Aminobutyric Acid (GABA) Improves Vegetative Growth, and the Physiological and Antioxidative Potential of Daucus Carota L. under Water Deficit Conditions

Contact Info

Product

Resources

About