Impact of phage predation on
            <i>P. aeruginosa</i>
            adhered to human airway epithelium: major transcriptomic changes in metabolism and virulence-associated genes

Brandão, Ana Catarina; Putzeys, Leena; Pires, Diana Priscila Penso; Voet, Marleen; Paeshuyse, Jan; Azeredo, Joana; Lavigne, Rob

doi:10.1080/15476286.2023.2216065

Cited by 5 publications

(3 citation statements)

References 76 publications

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“…Gene ontology enrichment analysis 55 of differentially expressed genes revealed that all treatments investigated resulted in the downregulation of locomotion processes, in accordance with a recent study using P. aeruginosa 56 , as well as cell localization, cell projection, organelle organization and response to external stimuli processes (Figure 6A, 6B and Supplementary files 4-6). Ciprofloxacin monotherapy also caused the downregulation of biological processes involved in cell communication, as well as the upregulation of RNA, catabolic, metabolic and ribosomal processes (Figure 6A and Supplementary file 4).…”

Section: Resultssupporting

confidence: 89%

Section: Synergistic Bactericidal Interactions Between Phage and Anti...supporting

confidence: 89%

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Phage-induced efflux down-regulation boosts antibiotic efficacy

Kraus,

Łapińska,

Chawla

et al. 2023

Preprint

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The interactions between a virus and its host vary in space and time and are affected by the presence of molecules that alter the physiology of either the host or the virus. Determining the dynamics of these interactions is paramount for predicting the fate of bacterial and phage populations and for designing rational phage-antibiotic therapies. We study the interactions between stationary phase Burkholderia thailandensis and the phage phi Bp-AMP1. Although heterogeneous genetic resistance to phage rapidly emerges in B. thailandensis, the presence of phage enhances the efficacy of three major antibiotic classes, the quinolones, the beta-lactams and the tetracyclines, but antagonizes tetrahydrofolate synthesis inhibitors. Enhanced antibiotic efficacy is underpinned by reduced antibiotic efflux in the presence of phage. This new phage-antibiotic therapy allows for eradication of stationary phase bacteria, whilst requiring reduced antibiotic concentrations, which is crucial for treating infections in sites where it is difficult to achieve high antibiotic concentrations.

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Section: Resultssupporting

confidence: 89%

Section: Synergistic Bactericidal Interactions Between Phage and Anti...supporting

confidence: 89%

Phage-induced efflux down-regulation boosts antibiotic efficacy

Kraus,

Łapińska,

Chawla

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Gene ontology enrichment analysis [ 46 ] of differentially expressed genes revealed that all treatments investigated resulted in the downregulation of locomotion processes, in accordance with a recent study using P . aeruginosa [ 47 ], as well as cell localization, cell projection, organelle organization and response to external stimuli processes (Fig 6A , 6B , S12 , S13 and S14 Files ). Ciprofloxacin monotherapy also caused the downregulation of biological processes involved in cell communication, as well as the upregulation of RNA, catabolic, metabolic and ribosomal processes ( Fig 6A and S12 File ).…”

Section: Resultsmentioning

confidence: 99%

Phage-induced efflux down-regulation boosts antibiotic efficacy

Kraus,

Fletcher,

Łapińska

et al. 2024

PLoS Pathog

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The interactions between a virus and its host vary in space and time and are affected by the presence of molecules that alter the physiology of either the host or the virus. Determining the molecular mechanisms at the basis of these interactions is paramount for predicting the fate of bacterial and phage populations and for designing rational phage-antibiotic therapies. We study the interactions between stationary phase Burkholderia thailandensis and the phage ΦBp-AMP1. Although heterogeneous genetic resistance to phage rapidly emerges in B. thailandensis, the presence of phage enhances the efficacy of three major antibiotic classes, the quinolones, the beta-lactams and the tetracyclines, but antagonizes tetrahydrofolate synthesis inhibitors. We discovered that enhanced antibiotic efficacy is facilitated by reduced antibiotic efflux in the presence of phage. This new phage-antibiotic therapy allows for eradication of stationary phase bacteria, whilst requiring reduced antibiotic concentrations, which is crucial for treating infections in sites where it is difficult to achieve high antibiotic concentrations.

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