Impact of Perfluorocarbons with Gas Transport Function on Growth of Phototrophic Microorganisms in a Free and Immobilized State and in Consortia with Bacteria

Abstract

The effects of the presence of perfluorocarbons (PFC) with a gas transport function in media with different phototrophic microorganisms on their growth rates and the accumulation of their biomass when using free and immobilized cells as inoculums were investigated. The significant increase in the average rate of biomass accumulation as well as levels of biomass accumulation in the presence of various PFCs were established for Chlorella vulgaris cells. When 1 g/L glycerol was introduced into the growth medium with PFCs and C. vulgaris cells, the increase in the rate of biomass accumulation was 9–32%. The maximum intracellular ATP concentrations corresponded to the combination of microalgae (Chlorella vulgaris) with bacterial cells (Pseudomonas esterophilus and Rhodoccus ruber) obtained with a mass ratio of 25:1. It provided for the formation of a consortium, which was able to accumulate the maximum amount of microalgae biomass for 3 days in the medium with PFCs and organophosphorus pesticide. The obtained data allow, on the one hand, predicting the growth of microalgae under environmental conditions in media with PFC pollution and, on the other hand, developing approaches to regulation of phototrophic microorganisms’ growth in order to obtain and use their high biomass yields for various purposes.

Recent advances and fundamentals of microalgae cultivation technology

“…1.3 P [148] Coelastrella sp. KGU-HN001 Comparison between algae monoculture and the coculture with N-fixing bacterium Rhodobacter sphaeroides with or without supplemental nitrogen.…”

“…[ 147 ] In the presence of perfluorocarbons and organophosphorus pesticide, co‐cultivating C. vulgaris with P seudomonas esterophilus and Rhodococcus ruber proved advantageous for biomass accumulation. [ 148 ] Consequently, microalgae development is facilitated by interactions with species‐specific bacterial consortiums.…”

Progressive Biocatalysts for the Treatment of Aqueous Systems Containing Pharmaceutical Pollutants

“…It should be noted that in order to stabilize microalgae cells in wastewater treatment processes, they can be immobilized ( Table 5 ) [ 76 , 77 , 78 , 79 , 80 ]. In this regard, the efficiency of the functioning of cells of the microalgae Nannochloropsis sp.…”

“…However, despite the high efficiency (up to 99%) of biological treatment using microalgae cells, this approach is longer in duration in comparison with enzymatic treatment of wastewater. Since microalgae and cyanobacteria mainly exist in stable consortia with other microorganisms in natural water sources, many researchers focus their attention on them [ 80 ].…”

“…Fundamentally, an effective approach to the creation of consortia for the destruction of xenobiotics is a directed combination of cultures based on the preliminary selection of active PCPs destructors and an understanding of the possible interaction between them [ 80 , 100 , 101 , 102 ].…”