Impact of Paenarthrobacter ureafaciens ZF1 on the soil enzyme activity and microbial community during the bioremediation of atrazine-contaminated soils

Abstract

Bioremediation of atrazine-contaminated soil is considered a safe and effective approach in removing contaminates from the soil. However, the effects of adding foreign organisms to assist bioremediation on soil environmental quality and ecosystem are unclear. Here, the ecological remediation potential of strain Paenarthrobacter ureafaciens ZF1 on atrazine-contaminated soil was investigated through miniature experiments using variations in soil enzymes and bacterial communities as indicators. The results showed that strain ZF1 accelerated atrazine degradation, which could completely degrade atrazine at concentrations of 100 mg·L− 1 atrazine within 2 h in liquid medium and could remove up to 99.3% of atrazine (100 mg·kg− 1 in soil) within 6 days. During soil bioremediation, atrazine promoted the activities of urease and cellulase, and inhibited the activities of sucrase and catalase, while the strain ZF1 significantly promoted the activities of these four enzymes. High-throughput sequencing of 16S rRNA genes showed that ZF1 affected the relative abundance and bacterial community structure, and promoted bacterial diversity and evenness. Furthermore, redundancy analysis revealed a certain correlation among the strain ZF1, atrazine residue, soil enzyme activity, and soil bacterial community. The strain ZF1 in this work demonstrated remarkable potential for ecological restoration, and can be an effective and environmentally friendly alternative in remediating atrazine-contaminated soil.

KEGG orthology prediction of bacterial proteins using natural language processing

“…Bacteria, ubiquitous microorganisms inhabiting diverse environments, play an indispensable role in shaping the biosphere and influencing human health [ 1 – 3 ]. Their sheer abundance and diversity underscore their significance in ecological processes, ranging from nutrient cycling to bioremediation [ 4 – 6 ]. Moreover, bacteria have been central to pivotal discoveries in the fields of genetics, molecular biology, and biotechnology, serving as model organisms for fundamental biological research.…”

Antifungal mechanism of volatile compounds emitted by Actinomycetota Paenarthrobacter ureafaciens from a disease-suppressive soil on Saccharomyces cerevisiae

“…Intriguingly, our previous study showed that Actinomycetota are major contributors to Rhizoctonia solani inhibition in soil ( 28 ), and from Rhizoctonia -suppressive soil, we isolated Paenarthrobacter ureafaciens , which belongs to the Actinomycetota phylum ( 29 , 30 ). P. ureafaciens is known as an indole acetic acid and siderophore producer ( 31 ) as well as an herbicide-degrading bacterium ( 32 , 33 ). Thus, Actinomycetota P. ureafaciens may impact plant growth and microbial communications in soil.…”