Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Nazareth, Talita Corrêa; Zanutto, Conrado Planas; Maass, Danielle; Souza, Antônio Augusto Ulson de; Souza, Selene de Arruda Guelli Ulson de Maria

doi:10.1016/j.jece.2021.105372

Cited by 5 publications

(5 citation statements)

References 45 publications

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“…Prior to measurements, the samples were properly diluted to fall within the calibration curve. Kim et al (1997), Nitschke and Pastore (2006), Kronemberger et al (2008), Silva et al (2015 and Nazareth et al (2021) also employed this method for determining biosurfactant concentration. Among the biocompounds synthetized during the cultivation which can lower the surface tension of the culture broth, surfactin is the most powerful in terms of minimum surface tension (Davis et al 1999;Chen et al 2015).…”

Section: Biomass Glucose and Surfactinmentioning

confidence: 99%

“…Davis et al (2001) suggested that overflow of the culture broth through the exhaust air line when high stirrer speeds are used could remove peptides that induce expression of the srfA gene, which is responsible for surfactin synthesis (Cosby et al 1998). Nazareth et al (2021) reported a volumetric productivity of 9.98 mg/L.h for a fermentation performed with 0.5 vvm and 150 rpm in a bioreactor coupled to a foam collector, which was lower than the one obtained in EXP4 (14.1 mg/L.h), although a higher aeration rate was employed by those authors. However, a higher surfactin concentration in the foam phase was observed compared to that found in the present study for the best condition.…”

Section: Kinetics Parameters Of Surfactin Productionmentioning

confidence: 99%

“…These microbial products represent promising alternatives to petrochemical surfactants, due to their lower toxicity and higher biodegradability (Winterburn et al 2010;Domingues et al 2017). In addition, they can be produced from renewable and cheap substrates, and exhibit good stability under extreme conditions of pH, temperature, and salinity (Desai and Banai 1997;Davis et al 1999;Nazareth et al 2021). Surfactin, a lipopeptide synthesized by Bacillus subtilis ATCC 21332 (Davis et al 1999), is one of the most effective biosurfactants available (Grangemard et al 2001;Chen et al 2015), since it can lower the surface tension of water from 72 to 27 mN/m, at concentrations as low as 0.005% (w/v) (Cooper et al 1981;Desai and Banai 1997).…”

Section: Introductionmentioning

confidence: 99%

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Effect of oxygen mass transfer coefficient (kLa) on surfactin production by Bacillus subtilis ATCC 21332

Martins,

Debon,

Schmidell

et al. 2024

Preprint

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Surfactin is one of the most effective biosurfactants available and has several interesting properties. However, the high cost and low yield associated with surfactin production have been major drawbacks preventing its wider industrial application. The use of intensive stirring and aeration, in order to increase surfactin synthesis, can cause excessive foaming, resulting in low levels of biosurfactant production inside the bioreactor. Therefore, the aim of this study was to evaluate the influence of the oxygen mass transfer coefficient (kLa) on surfactin productivity in a bioreactor integrated with a foam collector using low aeration rates. The experiments were carried out at 32°C, using a mineral salts medium containing 10 g/L of glucose, at constant pH 7.0. The results showed that the best surfactin production performance was achieved at kLa = 21.88 h− 1 (with 0.3 vvm aeration and 250 rpm stirring), which resulted in a maximum productivity of 14.1 mg/L.h and a maximum surfactin concentration of 443.1 mg/L in the foam. Despite loss of the culture broth through the exhaust air line, a very high surfactin concentration was reached in the foam phase, which can facilitate downstream processing and purification of the product. Besides, the best results regarding surfactin enrichment and recovery were obtained by using low aeration rate combined with high agitation rate.

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Section: Biomass Glucose and Surfactinmentioning

confidence: 99%

Section: Kinetics Parameters Of Surfactin Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of oxygen mass transfer coefficient (kLa) on surfactin production by Bacillus subtilis ATCC 21332

Martins,

Debon,

Schmidell

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…The use of agro-industrial residues is an interesting alternative for culture medium. Several agroindustrial residues rich in carbon have been successfully tested as substrate for surfactin production (Barros et al 2008), as potato waste (Fox and Bala 2000), whey powder (Cagri-Mehmetoglu et al 2012), rice straw (Zhu et al 2013), brewery waste (Paraszkiewicz et al 2018;Nazareth et al 2021). Cassava wastewater sanands out as a agro-industrial residue rich in carbon andfthe orssential nutrients to production of surfactin, in addition, it has advantages over other agro-industrial for being produced all year round and with low soil fertility requirements (de Oliveira Schmidt et al…”

Section: Surfactinmentioning

confidence: 99%

Cassava wastewater valorization for the production of biosurfactants: surfactin, rhamnolipids, and mannosileritritol lipids

Schmidt

Vasconcelos

Vicente

et al. 2022

World J Microbiol Biotechnol

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The global production of cassava was estimated at ca. 303 million tons. Due to this high production, the cassava processing industry (cassava flour and starch) generates approximately ca. 0.65 kg of solid residue and ca. 25.3 l of wastewater per kg of fresh processed cassava root. The composition of the liquid effluent varies according to its origin; for example, the effluent from cassava flour production, when compared to the wastewater from the starch processing, presents a higher organic load (ca. 12 times) and total cyanide (ca. 29 times). It is worthy to highlight the toxicity of cassava residues regarding cyanide presence, which could generate disorders with acute or chronic symptoms in humans and animals. In this sense, the development of simple and low-cost eco-friendly methods for the proper treatment or reuse of cassava wastewater is a challenging, but promising path. Cassava wastewater is rich in macro-nutrients (proteins, starch, sugars) and micro-nutrients (iron, magnesium), enabling its use as a low-cost culture medium for biotechnological processes, such as the production of biosurfactants. These compounds are amphipathic molecules synthesized by living cells and can be widely used in industries as pharmaceutical agents, for microbial-enhanced oil recovery, among others. Amongst these biosurfactants, surfactin, rhamnolipids, and mannosileritritol lipids show remarkable properties such as antimicrobial, biodegradability, demulsifying and emulsifying capacity. However, the high production cost restricts the massive biosurfactant applications. Therefore, this study aims to present the state of the art and challenges in the production of biosurfactants using cassava wastewater as an alternative culture medium.

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“…Depending on the microorganisms, BS/BE production can be induced by culture conditions. In fact, several factors, such as oxygen concentration, agitation, pH, temperature, culture density, and/or concentration of different salts, can have both positive and negative influences [6][7][8]. One of the most influential factors is the carbon source, which, based on its nature and concentration, can change not only the amount of BS/BE produced but also its type and characteristics [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A New Biosurfactant/Bioemulsifier from Gordonia alkanivorans Strain 1B: Production and Characterization

et al. 2022

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Biosurfactants and bioemulsifiers (BS/BE) are naturally synthesized molecules, which can be used as alternatives to traditional detergents. These molecules are commonly produced by microorganisms isolated from hydrocarbon-rich environments. Gordonia alkanivorans strain 1B was originally found in such an environment, however little was known about its abilities as a BS/BE producer. The goal of this work was to access the potential of strain 1B as a BS/BE producer and perform the initial characterization of the produced compounds. It was demonstrated that strain 1B was able to synthesize lipoglycoprotein compounds with BS/BE properties, both extracellularly and adhered to the cells, without the need for a hydrophobic inducer, producing emulsion in several different hydrophobic phases. Using a crude BS/BE powder, the critical micelle concentration was determined (CMC = 16.94 mg/L), and its capacity to reduce the surface tension to a minimum of 35.63 mN/m was demonstrated, surpassing many commercial surfactants. Moreover, after dialysis, emulsification assays revealed an activity similar to that of Triton X-100 in almond and sunflower oils. In benzene, the E24 value attained was 83.45%, which is 30% greater than that of the commercial alternative. The results obtained highlight for the presence of promising novel BS/BE produced by strain 1B.

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Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Cited by 5 publications

References 45 publications

Effect of oxygen mass transfer coefficient (kLa) on surfactin production by Bacillus subtilis ATCC 21332

Effect of oxygen mass transfer coefficient (kLa) on surfactin production by Bacillus subtilis ATCC 21332

Cassava wastewater valorization for the production of biosurfactants: surfactin, rhamnolipids, and mannosileritritol lipids

A New Biosurfactant/Bioemulsifier from Gordonia alkanivorans Strain 1B: Production and Characterization

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