Impact of oxidative stress on cellular biomechanics and rho signaling in C2C12 myoblasts

Sun, Shan; Wong, Sing Wan; Mak, Arthur F.T.; Cho, Michael

doi:10.1016/j.jbiomech.2014.09.036

Cited by 26 publications

(17 citation statements)

References 33 publications

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“…As expected, during induction by soluble factors, the stem cell mechanical properties are adjusted to those of fully differentiated cells. We found and reported that transient suppression of ERM expression by RNAi results in a temporary decrease of the cell elasticity and the membrane-cytoskeleton adhesion, mediated by the disassembly of the actin stress fibers and the focal adhesions in hMSCs and progenitor cells Titushki, 2013, Sun, 2014. The decrease in ERM protein expression in hMSCs on day 3 after ERM siRNA transfection correlates with the partial disassembly of the actin stress fibers and the focal adhesions.…”

Section: Lineage-dependent Changes In the Stem Cell Biomechanicsmentioning

confidence: 87%

“…The dense thin actin meshwork in load-bearing cells should yield low cell elastic modulus, but there might be multiple binding sites for coupling between the membrane and cytoskeleton. We identified a family of linkers proteins, including the well-characterized ezrin, radixin and moesin (ERM proteins) that can serve as the mechano-sensitive molecules regulating the strength of membrane-cytoskeletal coupling Sun, 2014;Titushkin, 2013]. In load-bearing cells, the sum of many weak interactions forms a stronger membrane-cytoskeleton linkage.…”

Section: Plasma Membrane Tensionmentioning

confidence: 99%

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Functional Stem Cell Biomechanics: Application of Biophysical Techniques and Multi-content 3D Image Analysis

Sun

Paul

Kanagaraj

et al. 2015

Biosystems &Amp; Biorobotics

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The mechanical properties of the cell -cytoskeleton elasticity, membrane tension, and adhesion strength -play an important role in the regulation of stem cell differentiation. While the cellular mechanical properties are significantly altered during stem cell specification to a particular phenotype, the complexity of events associated with transformation of these precursor cells leaves many questions unanswered about morphological, structural, proteomic, and functional changes in differentiating stem cells. However, control of cell behaviors might be feasible through manipulation of the cellular mechanical properties using external physical stimuli and manipulation of mechanically sensitive signaling molecules. Biomechanical regulation of stem cell differentiation can minimize the number of chemicals and growth factors that would otherwise be required for tissue engineering. Coupled with a thorough understanding of stem cell behavior, both experimentally and computationally, development of more effective approaches is a feasible way to expand stem cells and to regulate their phenotypic commitment. We recently developed a high-content/high-throughput screening algorithm that offers significant improvements in 3D quantitative analysis at the single cell level. A consistent pattern observed in all types of stem cell differentiation indicates the cytoskeleton remodeled significantly before lineagespecific cellular changes occurred. This demonstrates that cellular mechanical transformations are a precursor to stem cell differentiation and to phenotypic functionality.

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Section: Lineage-dependent Changes In the Stem Cell Biomechanicsmentioning

confidence: 87%

Section: Plasma Membrane Tensionmentioning

confidence: 99%

Functional Stem Cell Biomechanics: Application of Biophysical Techniques and Multi-content 3D Image Analysis

Sun

Paul

Kanagaraj

et al. 2015

Biosystems &Amp; Biorobotics

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“…The ERM (ezrin, radixin, moesin) linker proteins provide important linkages between the plasma membrane and the underlying cell cortex (Neisch and Fehon 2011). Membranecortex linker proteins ERM expressions also decreased in a concentration-dependent manner with H 2 O 2 (Sun et al 2014). High intensity of oxidative stress could weaken the actin stress fiber and actin cortex together.…”

Section: Discussionmentioning

confidence: 99%

“…Actin filament cytoskeleton underwent observable changes under oxidative insults (Zhu et al 2005). Oxidative stress also affected the RhoA signaling pathway, actin filament polymerization, linker proteins expression and cell stiffness in myoblasts (Sun et al 2014;Wong et al 2014). Resistance against compressive damage was compromised in myoblasts under chronic and high oxidative stress (Yao et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of oxidative stress-induced changes in the actin cytoskeletal structure on myoblast damage under compressive stress: confocal-based cell-specific finite element analysis

Yao

Lacroix

Mak

2016

Biomech Model Mechanobiol

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Muscle cells are frequently subjected to both mechanical and oxidative stresses in various physiological and pathological situations. To explore the mechanical mechanism of muscle cell damage under loading and oxidative stresses, we experimentally studied the effects of extrinsic hydrogen peroxides on the actin cytoskeletal structure in C2C12 myoblasts and presented a finite element (FE) analysis of how such changes in the actin cytoskeletal structure affected a myoblast's capability to resist damage under compression. A confocal-based cell-specific FE model was built to parametrically study the effects of stress fiber density, fiber cross-sectional area, fiber tensile prestrain, as well as the elastic moduli of the stress fibers, actin cortex, nucleus and cytoplasm. The results showed that a decrease in the elastic moduli of both the stress fibers and actin cortex could increase the average tensile strain on the actin cortex-membrane structure and reduce the apparent cell elastic modulus. Assuming the cell would die when a certain percentage of membrane elements were strained beyond a threshold, a lower elastic modulus of actin cytoskeleton would compromise the compressive resistance of a myoblast and lead to cell death more readily. This model was used with a Weibull distribution function to successfully describe the extent of myoblasts damaged in a monolayer under compression.

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“…최근 골격근 세포 손상의 주요한 기전으로 산화적 스트레스의 중 요성이 인식되면서 [8][9][10] , C2C12 세포는 근기능 손상을 보호하기 위한 다양한 약물의 발굴을 위한 모델로서 널리 사용되고 있다 [11][12][13] . Ⅱ.…”

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Protective Effects of Ethanol Extract Mixtures of Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus against Oxidative Stress-induced Damage in C2C12 Murine Myoblasts

Choi¹,

Hwangbo²,

Kim³

et al. 2017

Herbal Formula Science

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Impact of oxidative stress on cellular biomechanics and rho signaling in C2C12 myoblasts

Cited by 26 publications

References 33 publications

Functional Stem Cell Biomechanics: Application of Biophysical Techniques and Multi-content 3D Image Analysis

Functional Stem Cell Biomechanics: Application of Biophysical Techniques and Multi-content 3D Image Analysis

Effects of oxidative stress-induced changes in the actin cytoskeletal structure on myoblast damage under compressive stress: confocal-based cell-specific finite element analysis

Protective Effects of Ethanol Extract Mixtures of Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus against Oxidative Stress-induced Damage in C2C12 Murine Myoblasts

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