Impact of oil type and pH value on oil-in-water emulsions stabilized by egg yolk granules

Gmach, Oliver; Bertsch, Annalisse; Bilke-Krause, C.; Kulozik, Ulrich

doi:10.1016/j.colsurfa.2019.123788

Cited by 36 publications

(27 citation statements)

References 32 publications

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“…From pH 2.0 to 3.0, the absolute value of zeta potential of EYGs was much larger than that at other pH values, which might be due to the different aggregation states of EYGs under different pH. This phenomenon was consistent with the previous study [ 20 ].…”

Section: Resultssupporting

confidence: 91%

“…However, in the absence of NaCl salt ions, the isoelectric point of the EYGs was 4.0 instead of 5.0 [ 15 ]. Similarly, it had also been found that the isoelectric point of the EYGs was between 4.0 and 5.0 with the addition of 0.15 M NaCl in solution [ 20 ]. This result might be due to the increase in environmental NaCl ionic strength changing the ionization state of protein groups, which affected the number of surface charges.…”

Section: Resultsmentioning

confidence: 99%

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Formation of Natural Egg Yolk Granule Stabilized Pickering High Internal Phase Emulsions by Means of NaCl Ionic Strength and pH Change

Xia

Zhang

et al. 2022

Foods

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Pickering high internal phase emulsions (HIPEs) are gel-like concentrated emulsions that have the potential to be an alternative to partially hydrogenated oil (PHO). In this study, egg yolk granules (EYGs), natural complexes of protein and lipid isolated from egg yolk, were used as an emulsifier to prepare Pickering HIPEs. Gel-like HIPEs with an oil phase volume fraction of 85% and with an emulsifier concentration of only 0.5% could be prepared by using EYGs as an emulsifier. The EYGs were able to form stable HIPEs at NaCl ionic strengths over 0.2 M and at pH over 5.0 with NaCl ionic strength of 0.3 M. The EYGs, which could stabilize HIPEs, were easily to adsorb and cover the oil-water interface to form emulsion droplets with small particle size. In addition, interacting EYGs in the aqueous phase formed a continuous network structure, and the oil droplets packed closely, exhibiting high elasticity and shear thinning behavior. Furthermore, the formed HIPEs had suitable storage stability with no significant changes in appearance and microstructure after storage for 60 days. This work can transform traditional oils from liquid-like to solid-like by using EYGs to enrich food processing diversity and improve the storage stability of oils while reducing the intake of PHO and providing a healthier diet for consumers.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Formation of Natural Egg Yolk Granule Stabilized Pickering High Internal Phase Emulsions by Means of NaCl Ionic Strength and pH Change

Xia

Zhang

et al. 2022

Foods

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“…Samples were diluted with 0.5% sodium dodecyl sulfate (Serva; Heidelberg, Germany) solution (1:10, vol/vol) to separate agglomerates and measure single lipid droplets. 19 From the particle-size distributions, mean diameters were extracted.…”

Section: Phantom Manufacturingmentioning

confidence: 99%

“…17 Instead, a stimulated echo-based (STE) preparation may allow to probe diffusion restriction effects at long diffusion times, and a single-shot turbo spin-echo (TSE) readout could reduce the sensitivity to chemical shift artifacts. 18 Therefore, the purpose of the present work was to (1) develop a DW-STE-prepared single-shot TSE sequence for measuring lipid diffusion using a clinical 3T system, (2) develop the processing for lipid droplet size mapping, (3) validate the methodology in water-fat emulsions by comparing the MR results with laser deflection measurements (methodology to measure lipid particle-size distributions 19 ) and ( 4) to investigate the methodology in ex vivo human AT samples by comparing the MR results with histology.…”

Section: Introductionmentioning

confidence: 99%

Lipid droplet–size mapping in human adipose tissue using a clinical 3T system

Weidlich

Honecker

Boehm

et al. 2021

Magnetic Resonance in Med

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Purpose To develop a methodology for probing lipid droplet sizes with a clinical system based on a diffusion‐weighted stimulated echo–prepared turbo spin‐echo sequence and to validate the methodology in water–fat emulsions and show its applicability in ex vivo adipose‐tissue samples. Methods A diffusion‐weighted stimulated echo–prepared preparation was combined with a single‐shot turbo spin‐echo readout for measurements at different b‐values and diffusion times. The droplet size was estimated with an analytical expression, and three fitting approaches were compared: magnitude‐based spatial averaging with voxel‐wise residual minimization, complex‐based spatial averaging with voxel‐wise residual minimization, and complex‐based spatial averaging with neighborhood‐regularized residual minimization. Simulations were performed to characterize the fitting residual landscape and the approaches’ noise performance. The applicability was assessed in oil‐in‐water emulsions in comparison with laser deflection and in ten human white adipose tissue samples in comparison with histology. Results The fitting residual landscape showed a minimum valley with increasing extent as the droplet size increased. In phantoms, a very good agreement of the mean droplet size was observed between the diffusion‐weighted MRI‐based and the laser deflection measurements, showing the best performance with complex‐based spatial averaging with neighborhood‐regularized residual minimization processing (R2/P: 0.971/0.014). In the human adipose‐tissue samples, complex‐based spatial averaging with neighborhood‐regularized residual minimization processing showed a significant correlation (R2/P: 0.531/0.017) compared with histology. Conclusion The proposed acquisition and parameter‐estimation methodology was able to probe restricted diffusion effects in lipid droplets. The methodology was validated using phantoms, and its feasibility in measuring an apparent lipid droplet size was demonstrated ex vivo in white adipose tissue.

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“…Emulsification was carried out using a colloid mill (Labor-Pilot 2000/4, IKA-Werke) set to 3000, 5000, 8000, and 12,000 rpm to obtain different lipid droplet sizes within the water matrix as described earlier (30,33,34). The generated lipid droplet size spectrum was determined using a laser diffraction particle size analyzer (( Mastersizer 2000 with Hydro 2000S dispersing unit, Malvern Instruments).…”

Section: Lipid Droplet Phantom Studiesmentioning

confidence: 99%

Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue

Honecker

Ruschke

Seeliger

et al. 2021

Preprint

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Adipose tissue is an organ with great plasticity and its hypertrophic expansion is associated with adipocyte dysfunction. How changes in adipocyte morphology are linked to gene expression and which cellular functions are affected remains elusive. We show that adipocyte hypertrophy is associated with transcriptomic changes using RNA-Seq data obtained from adipose tissue and size-separated adipocytes. Genes involved in oxidative phosphorylation, protein biosynthesis and fatty acid metabolism were down-regulated in large adipocytes while genes involved in inflammation were upregulated. For mitochondrial function, a reduction in the expression of thermogenesis related genes and estimated brown/beige adipocyte content was observed in individuals with large adipocytes. As a novel finding the total adipose tissue fatty acid composition was dependent on cell size and depot. MR spectroscopy methods for clinical scanning were developed to characterize adipocyte size and fatty acid composition in a fast and non-invasive manner. Together, the present data provides mechanistic insights on how adipocyte hypertrophy contributes to the manifestation of metabolic disease at the molecular and cellular level. MR spectroscopy was identified as a promising technique for an in-parallel assessment of adipose morphology and fatty acid composition allowing to translate our findings into an improved, non-invasive phenotyping of adipose tissue.

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Impact of oil type and pH value on oil-in-water emulsions stabilized by egg yolk granules

Cited by 36 publications

References 32 publications

Formation of Natural Egg Yolk Granule Stabilized Pickering High Internal Phase Emulsions by Means of NaCl Ionic Strength and pH Change

Formation of Natural Egg Yolk Granule Stabilized Pickering High Internal Phase Emulsions by Means of NaCl Ionic Strength and pH Change

Lipid droplet–size mapping in human adipose tissue using a clinical 3T system

Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue

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