Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly

Shirai, Akira; Kawaguchi, Takayuki; Shimojo, Hideaki; Muramatsu, Daisuke; Ishida-Yonetani, Mayumi; Nishimura, Yoshifumi; Kimurâ, Hiroshi; Nakayama, Jun‐ichi; Shinkai, Yoichi

doi:10.7554/elife.25317

Cited by 68 publications

(69 citation statements)

References 56 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SUV39H1 mediated DNMT3A expression through up-regulating H3K9me3 in cervical cancer cells SUV39H1 is the histone methyltransferase (HMTase) of histone H3 lysine 9 trimethylation (H3K9me3) [16]. SUV39H1 recognizes trimethylated H3K9 (H3K9me3) via its chromodomain (CD), and enriched H3K9me3 afterwards [17]. The H3K9me3 and DNMT3A both expressed in SiHa, HeLa and C33A cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer

Zhang

Tian

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Methylation of histone 3 at lysine 9 (H3K9) and DNA methylation are epigenetic marks correlated with genes silencing. The tumor microenvironment significantly influences therapeutic responses and clinical outcomes. The epigenetic-regulation mechanism of the costimulatory factors Tim-3 and galectin-9 in cervical cancer remains unknown. Methods: The methylation status of HAVCR2 and LGALS9 were detected by MS-PCR in cervical cancer tissues and cell lines. The underlying molecular mechanism of SUV39H1-DNMT3A-Tim-3/galectin-9 regulation was elucidated using cervical cancer cell lines containing siRNA or/and over-expression system. Confirmation of the regulation of DNMT3A by SUV39H1 used ChIP-qPCR.Results: SUV39H1 up-regulates H3K9me3 expression at the DNMT3A promoter region, which in turn induced expression of DNMT3A in cervical cancer. In addition, the mechanistic studies indicate that DNMT3A mediates the epigenetic modulation of the HAVCR2 and LGALS9 genes by directly binding to their promoter regions in vitro. Moreover, in an in vivo assay, the expression profile of SUV39H1 up-regulates the level of H3K9me3 at the DNMT3A promoter region was found to correlate with Tim-3 and galectin-9 cellular expression level. Conclusion: These results indicate that SUV39H1-DNMT3A is a crucial Tim-3 and galectin-9 regulatory axis in cervical cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer

Zhang

Tian

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…αSAT ncRNAs also seem to regulate spindle microtubule attachment and sister chromatid disjunction through association with AURORA B proteins [182]. These molecules were further shown to be associated with the SUV39H1 histone methyltransferase, thereby suggesting a regulatory function in heterochromatin maintenance [183][184][185].…”

Section: Transcribing Satdnas: Targeting Genomic Functionsmentioning

confidence: 97%

Decoding the Role of Satellite DNA in Genome Architecture and Plasticity—An Evolutionary and Clinical Affair

Louzada

Lopes

Ferreira

et al. 2020

Genes

View full text Add to dashboard Cite

Repetitive DNA is a major organizational component of eukaryotic genomes, being intrinsically related with their architecture and evolution. Tandemly repeated satellite DNAs (satDNAs) can be found clustered in specific heterochromatin-rich chromosomal regions, building vital structures like functional centromeres and also dispersed within euchromatin. Interestingly, despite their association to critical chromosomal structures, satDNAs are widely variable among species due to their high turnover rates. This dynamic behavior has been associated with genome plasticity and chromosome rearrangements, leading to the reshaping of genomes. Here we present the current knowledge regarding satDNAs in the light of new genomic technologies, and the challenges in the study of these sequences. Furthermore, we discuss how these sequences, together with other repeats, influence genome architecture, impacting its evolution and association with disease.

show abstract

“…It can bind to DNA independent of its H3K9 methylation status, in the presence of chromatin-bound major satellite transcripts. These transcripts thereafter target Suv39h enzymes to na€ ıve nucleosomes at the onset of heterochromatin formation [42]. This finding was corroborated by another independent study, where using RNase treatment and RNA polymerase inhibition, it was shown that in human cells, Suv39h1/h2 gets targeted to heterochromatin by binding to the repetitive a-satellite transcripts in both the mitotic and interphase stages [43].…”

Section: Centromeric and Pericentromeric Transcripts In Heterochromatmentioning

confidence: 62%

Heterochromatic hues of transcription—the diverse roles of noncoding transcripts from constitutive heterochromatin

Saha

Mishra

2019

The FEBS Journal

View full text Add to dashboard Cite

Constitutive heterochromatin has been canonically considered as transcriptionally inert chromosomal regions, which silences the repeats and transposable elements (TEs), to preserve genomic integrity. However, several studies from the last few decades show that centromeric and pericentromeric regions also get transcribed and these transcripts are involved in multiple cellular processes. Regulation of such spatially and temporally controlled transcription and their relevance to heterochromatin function have emerged as an active area of research in chromatin biology. Here, we review the myriad of roles of noncoding transcripts from the constitutive heterochromatin in the establishment and maintenance of heterochromatin, kinetochore assembly, germline epigenome maintenance, early development, and diseases. Contrary to general expectations, there are active protein‐coding genes in the heterochromatin although the regulatory mechanisms of their expression are largely unknown. We propose plausible hypotheses to explain heterochromatic gene expression using Drosophila melanogaster as a model, and discuss the evolutionary significance of these transcripts in the context of Drosophilid speciation. Such analyses offer insights into the regulatory pathways and functions of heterochromatic transcripts which open new avenues for further investigation.

show abstract

Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly

Cited by 68 publications

References 56 publications

SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer

SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer

Decoding the Role of Satellite DNA in Genome Architecture and Plasticity—An Evolutionary and Clinical Affair

Heterochromatic hues of transcription—the diverse roles of noncoding transcripts from constitutive heterochromatin

Contact Info

Product

Resources

About