Impact of neutron radiation on the viability of tumor cells cultured in the presence of boron-10 isotope

Volkova, Olga Y.; Mechetina, Ludmila V.; Taranin, Alexander V.; Zaboronok, Alexander; Nakai, Kei; Lezhnin, S. I.; Фролов, С. А.; Kasatov, D. A.; Makarov, A. N.; Sorokin, Igor; Sycheva, T. V.; Shchudlo, Ivan; Taskaev, S. Yu.

doi:10.20862/0042-4676-2016-97-5-283-288

Cited by 11 publications

(16 citation statements)

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“…The results of the clonogenic test showed that neutron irradiation of tumor cells with boron leads to a significant suppression of their survivability that confirms the quality of generated neutron beam generated by Institute of nuclear physics demanding for BCT (Fig. 2) [48].…”

Section: Current Status Of Research On the Source Of Neutrons Inp Sb Rassupporting

confidence: 68%

Perspectives of boron-neutron capture therapy of malignant brain tumors

Kanygin

Кичигин

Кривошапкин

et al. 2017

AIP Conference Proceedings

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Boron neutron capture therapy (BN T) is characterized by a selective effect directly on the cells of malignant tumors. The carried out research showed the perspective of the given kind of therapy concerning malignant tumors of the brain. However, the introduction of BNCT into clinical practice is hampered by the lack of a single protocol for the treatment of patients and the difficulty in using nuclear reactors to produce a neutron beam. This problem can be solved by using a compact accelerator as a source of neutrons, with the possibility of installation in a medical institution. Such a neutron accelerator for BN T was developed at Budker Institute of Nuclear Physics, Novosibirsk. A neutron beam was obtained on this accelerator, which fully complies with the requirements of BN T, as confirmed by studies on cell cultures and experiments with laboratory animals. The conducted experiments showed the relative safety of the method with the absence of negative effects on cell cultures and living organisms, and also confirmed the effectiveness of BN T for malignant brain tumors.

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Section: Current Status Of Research On the Source Of Neutrons Inp Sb Rassupporting

confidence: 68%

Perspectives of boron-neutron capture therapy of malignant brain tumors

Kanygin

Кичигин

Кривошапкин

et al. 2017

AIP Conference Proceedings

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“…To more accurately model parameters for future studies, we compared the response of several cell lines, including U251MG—a human glioma, and two normal cell lines. First, we chose the CHO-K1 line previously used in our recent study on the accumulation of new boron compounds, and then also examined the V79 cell line (lung fibroblasts that represent normal tissue cells and are generally accepted in the literature as suitable models [ 1 , 28 , 32 ]).…”

Section: Discussionmentioning

confidence: 99%

Radiobiological response of U251MG, CHO-K1 and V79 cell lines to accelerator-based boron neutron capture therapy

Sato

Zaboronok

Yamamoto

et al. 2017

Journal of Radiation Research

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In the current article, we provide in vitro efficacy evaluation of a unique accelerator-based neutron source, constructed at the Budker Institute of Nuclear Physics (Novosibirsk, Russian Federation), for boron neutron capture therapy (BNCT), which is particularly effective in the case of invasive cancers. U251MG, CHO-K1 and V79 cells were incubated and irradiated in various concentrations of boric acid with epithermal neutrons for 2–3 h in a plexiglass phantom, using 2.0 MeV proton energy and 1.5–3.0 mA proton current, resulting in a neutron fluence of 2.16 × 1012 cm−2. The survival curves of cells loaded with boron were normalized to those irradiated without boron (to exclude the influence of the fast neutron and gamma dose components) and fit to the linear–quadratic (LQ) model. Colony formation assays showed the following cell survival rates (means ± SDs): CHO-K1: 0.348 ± 0.069 (10 ppm), 0.058 ± 0.017 (20 ppm), 0.018 ± 0.005 (40 ppm); V79: 0.476 ± 0.160 (10 ppm), 0.346 ± 0.053 (20 ppm), 0.078 ± 0.015 (40 ppm); and U251MG: 0.311 ± 0.061 (10 ppm), 0.131 ± 0.022 (20 ppm), 0.020 ± 0.010 (40 ppm). The difference between treated cells and controls was significant in all cases (P < 0.01) and confirmed that the neutron source and irradiation regimen were sufficient for control over cell colony formation. We believe our study will serve as a model for ongoing in vitro experiments on neutron capture therapy to advance in this area for further development of accelerator-based BNCT into the clinical phase.

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“…The authors calculated the value of fluence at which blisters appeared in Cu under 2 MeV proton irradiation based on the value at 180 keV and the fluence dependence on the energy as E 0.4 given in [9] to be 2 × 10 19 cm −2 ([2], p. 81). We experimentally obtained a similar value of 1.7 × 10 19 cm −2 ([2], p. 82) after neutron generation for the purpose of biological studies [11]. In conclusion, we say that there is no proper agreement of the threshold fluence for blistering in the literature.…”

Section: Introductionmentioning

confidence: 75%

In Situ Observations of Blistering of a Metal Irradiated with 2-MeV Protons

Badrutdinov

Bykov

Gromilov

et al. 2017

Metals

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A vacuum-insulated tandem accelerator was used to observe in situ blistering during 2-MeV proton irradiation of metallic samples to a fluence of up to 6.7 × 10 20 cm −2 . Samples consisting of copper of different purity, tantalum and tantalum-copper compounds were placed on the proton beam path and forced to cool. The surface state of the samples was observed using a charge-coupled device camera with a remote microscope. Thermistors, a pyrometer and an infrared camera were applied to measure the temperature of the samples during irradiation. After irradiation, the samples were analyzed on an X-ray diffractometer, laser and electron microscopes. The present study describes the experiment, presents the results obtained and notes their relevance and significance in the development of a lithium target for an accelerator-based neutron source, for use in boron neutron capture therapy of cancer.

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Impact of neutron radiation on the viability of tumor cells cultured in the presence of boron-10 isotope

Cited by 11 publications

References 0 publications

Perspectives of boron-neutron capture therapy of malignant brain tumors

Perspectives of boron-neutron capture therapy of malignant brain tumors

Radiobiological response of U251MG, CHO-K1 and V79 cell lines to accelerator-based boron neutron capture therapy

In Situ Observations of Blistering of a Metal Irradiated with 2-MeV Protons

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