“…On the other hand, specially conserved in anhydrobiotic cyanobacterial MTs, serine residues (Ser-19 and Ser-33) can provide an enhanced molecular stability (Emoto et al, 1996). Such stabilizing effect was described in Pseudomonas fluorescens (Cd4PflQ2 MT), in which Ser29 has an essential role as an N-cap for a short alpha-helix, forming two hydrogen bonds, one from its carbonyl oxygen to the amide proton of Ala33 and a second one from the side-chain oxygen to the amide proton of Ala31 (Habjanič et al, 2020). However, most of these conserved non-coordinating residues (V4, T5, M7, K8, A10, L15, V18, E21, A23, K26, D27, K29, Y32, G39, G46, and G51) do not have well characterized functions in MT.…”