Impact of molasses and microbial inoculants on fermentation quality, aerobic stability, and bacterial and fungal microbiomes of barley silage

Abstract

This study aimed to investigate the effects of microbial inoculants (L) and molasses (M) on the bacterial and fungal microbiomes of barley silage after the aerobic stage. The addition of molasses and microbial inoculants improved the aerobic stability of barley silage. The ML silage, which had a low pH value and high lactic and acetic acid contents, remained aerobically stable for more than 216 h. The ML silage exhibited low bacterial and high fungal diversities. Microbial inoculants and molasses enriched the abundance of Lactobacillus in silage after aerobic exposure. The enrichment of L. buchneri was significant in ML silage at days 5 and 7 during the aerobic stage. The abundance of harmful microorganisms, such as aerobic bacterial including Acinetobacter, Providencia, Bacillus, and yeasts including Issatchenkia, Candida, and Kazachstania, were suppressed in ML silage. M and L had an impact on bacterial and fungal microbes, resulting in the improvement of fermentation quality and reduction of aerobic spoilage in barley silage.

Silage making of maize stover and banana pseudostem under South Ethiopian conditions: evolution of pH, dry matter and microbiological profile

“…The significant difference ( P = 0.000) between the two banana‐based silages can only be related to the addition of the molasses that might have replaced the lost water soluble carbohydrates during squeezing (Ni et al ., 2017; Bernardes et al ., 2018). While some fungi and yeasts can grow at low pH values, a rapid decrease in pH to a value below 4.5 likely contributes to the inhibition of spoilage and pathogenic microorganisms (Hu et al ., 2018; Liu et al ., 2020).…”

“…These two practices can be hypothesized to reduce the soluble carbohydrate content (WSC) available for microbial fermentation. Therefore, addition of molasses can substitute the WSC and enhance lactic acid fermentation (McDonald et al ., 1991; Ni et al ., 2018; Liu et al ., 2020; Shiau et al ., 2020). To the best of our knowledge, there is only one study in China (Wang et al ., 2016) reporting on the feasibility of ensiling banana pseudostem for feed purposes, and bacterial profiles have not yet been determined by Illumina sequencing.…”

Lactobacillus plantarum and molasses alter dynamic chemical composition, microbial community, and aerobic stability of mixed (amaranth and rice straw) silage

“…Similarly, Lactobacillus was the dominant genus in mulberry leaf silage treated with L. casei and sucrose 28 . Adding 2% molasses and LAB in barley silage enriched the abundance of Lactobacillus and suppressed the abundance of harmful microorganisms 35 . Enterobacteria are facultative anaerobes (i.e.…”

Dual sensor measurement shows that temperature outperforms pH as an early sign of aerobic deterioration in maize silage

“…2 cm) were air inlets for the aerobic-exposure measures when rubber stoppers were removed. Ex situ determination of silage pH followed a standard protocol of extraction of 25 g of maize silage with 0.225 L deionized water for 30 min 4 , 14 , 23 .…”

“…Since utilization of acids by aerobic microorganisms leads to a rise of silage pH, the pH of a silage mass reflects the extent of aerobic deterioration 14 . Previous studies of aerobic responses of silage pH used laboratory mini-silos 7 , 15 – 18 and farm silos 5 , 10 , 19 – 23 , both with invasive sub-sampling followed by liquid extraction of silage samples.…”