Impact of miRNAs expression modulation on the methylation status of breast cancer stem cell-related genes

Elosaily, Heba H.; Ibrahim, I. K. A.; Essawi, Mona; Salem, Sohair

doi:10.1007/s12094-020-02542-0

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…NDN methylation was also observed in endometrial carcinoma by Liu [ 38 ]. Osaily [ 39 ] reported that miRNA can regulate the methylation of genes associated with breast cancer stem cells. Interestingly, a meta-analysis [ 40 ] indicated that the miR-200 family can cause NDN methylation, which is consistent with our results, and suggested that further exploration of the changes in NDN due to miR-200c is necessary.…”

Section: Discussionmentioning

confidence: 99%

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Wang

et al. 2022

Bioengineered

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MicroRNA-200c (miR-200c) generally acts as a tumor suppressor in multiple cancer types and a promising therapeutic target in tumorigenesis. However, only a few studies have explained the role of miR-200c in the development of osteosarcoma (OS). In this study, we investigated the role of miR-200c in OS progression and identified the regulatory pathway protein NDN involved in inhibiting the occurrence and development of OS. Firstly, we found that miR-200c is downregulated in OS cells and tissues. As well, in vitro and in vivo experiments showed that upregulating miR-200c inhibits the proliferation, invasion, metastasis of Saos-2 cells, promotes the apoptosis of Saos-2 cells and suppresses tumor growth in mice, indicating miR-200c plays a major role in regulating the OS progression. Furthermore, bioinformatics analysis showed that an anti-tumor protein, necdin (NDN), might be a potential target by miR-200c. To verify this hypothesis, we measured the expression level of NDN in OS cells and tissues and found NDN is downregulated, suggesting NDN is functional in OS progression. Moreover, we found that the expression levels of NDN and miR-200c in in vivo and in vitro experiments were positively correlated. However, the results of dual-luciferase reporter gene experiment showed miR-200c does not directly act on the 3ʹ untranslated region (UTR) of NDN gene, indicating that NDN might be an important pathway protein which regulates OS progression in the presence of miR-200c. Therefore, miR-200c/NDN could be potential targets for developing effective treatment against OS.

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Section: Discussionmentioning

confidence: 99%

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Wang

et al. 2022

Bioengineered

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“…Breast cancer stem cells (BCSCs) were first described in a publication in 2004 [8,64]. They were identified and isolated based on their cell surface marker expressions CD44 + , CD24 -/low and epithelial surface antigen (ESA).…”

Section: Fig 2 Adapted Schematic Illustrating Carcinogenesis Arising ...mentioning

confidence: 99%

“…They were identified and isolated based on their cell surface marker expressions CD44 + , CD24 -/low and epithelial surface antigen (ESA). The expression of high levels of aldehyde dehydrogenase 1 (ALDH1) on BCSCs and the analysis of CD44 and ALDH1 on the cells in the serum showed high level of CD44 expressions were associated with reduced cancer survival when compared to the low serum expression of CD44 [9,64]. Thus, CD44 is a cell surface antigen that is important for cell-to-cell interaction, migration, and adhesion.…”

Section: Fig 2 Adapted Schematic Illustrating Carcinogenesis Arising ...mentioning

confidence: 99%

The Role of MicroRNAs Regulated Breast Cancer Stem Cells in the Pathogenesis, Prognosis and Aggressiveness of Breast Cancer

Agboola

Okikiade

Afolayan-Oloye

2023

AIR

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Breast cancer is a leading cause of cancer related death among women worldwide and it is a complex disease characterized by its heterogeneity. The heterogenous characteristic of the breast cancer is responsible for its aggressiveness and treatment resistance. Breast cancer stem cells (BCSCs) are population of heterogenous cells with increased propensity for proliferation, differentiation, migration, invasion and mammosphere formation with increased resistance to treatment. They have ability to initiate and propagate cancer cells population and share similarity with the normal stem cells in relation to their expressions of cell surface markers; Cluster of Differentiation 44 positive (CD44+), Cluster of Differentiation 24 negative/low (CD24-/low) and Alkaline dehydrogenase 1 (ALDH1). MicroRNAs (miRNAs) are responsible for the RNA silencing and post-transcriptional regulation of the gene expression. MicroRNAs (miRNAs) control gene expression by acting as oncogenes or tumor suppressor genes. The epigenetic mechanisms are used for the regulation of miRNAs expression in the breast cancer, and this can be targeted to reverse the cancer progression. This review aims to provide an overview of the pathogenic mechanism of the microRNA’s expressions on the breast cancer stem cells in controlling the prognosis and aggressiveness of breast cancer disease. This is done by highlighting the regulatory mechanism of miRNA expressions on the BCSCs. Dysregulation of the MicroRNAs expression in the breast cancer stem cells and upregulated miRNAs expression were identified in the breast cancer stem cells (BCSCs), including miR-10b, miR-21, miR-155, miR-181, miR-183, miR-210 and miR-221/222, and the downregulated miRNAs expression in BCSCs include Let-7, miR-22, miR-30e, miR-31, miR-103/107, miR-200, miR-205, miR-335, miR-449a, miR-519c, miR-600, miR-708, miR-760. Studies showed association between the miRNA expression and breast cancer metastasis and aggressiveness. MicroRNAs were noticed to have a regulatory effect on neovascularization, drug resistance, and cancer metastasis. They can be used as predictive indicators in determining prognosis of breast cancer following cancer treatment and miRNAs expression can be used to determine the metastatic BCSCs for an efficient targeting mechanism and identification of the metastatic cancer cells. The systematic literature review aims at providing insight into the potential roles of microRNA in oncology especially breast cancers. The data for this study review were obtained from the Medline on OvidSP, includes PubMed of the US National Library of Medicine and the search was done through the University of Bristol Library services. This is to provide an overview of the pathogenic mechanism of microRNAs regulatory effect on the breast cancer stem cells in controlling the prognosis and aggressiveness of breast cancer disease. The review will be providing highlight of the dysregulation of oncogenic and tumor suppressor microRNAs in the breast cancer stem cells and highlighting the roles of miRNAs regulated BCSCs in the pathogenesis, prognosis, and aggressiveness of the breast cancer. We further discuss the biogenesis of miRNAs, regulatory roles of miRNAs on BCSCs, and then summarize the mechanism of miRNAs regulated BCSCs.

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“…Besides, miR-770-5p repressed invasion and EMT via inhibition of DNMT3A and restoration of E-cadherin in MDA-MB-231 TNBC cells ( Noyan et al, 2021 ). Moreover, miR-203 and miR-150 can regulate the expression of DNMT3A and DNMT3B in breast cancer cells, resulting in regulation of CD44, ALDH1A3, OCT3/4, SOX2 expression, which are critical for breast cancer stem cell development ( El-Osaily et al, 2021 ). Clearly, numerous miRNAs regulate the expression of DNMT3A and DNMT3B in breast cancer.…”

Section: Introductionmentioning

confidence: 99%

DNMT3A and DNMT3B in Breast Tumorigenesis and Potential Therapy

Man

Wang

et al. 2022

Front. Cell Dev. Biol.

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Breast cancer has become a leading cause of cancer-related deaths in women worldwide. DNA methylation has been revealed to play an enormously important role in the development and progression of breast cancer. DNA methylation is regulated by DNA methyltransferases (DNMTs), including DNMT1, DNMT2, and DNMT3. DNMT3 family has three members: DNMT3A, DNMT3B, and DNMT3L. The roles and functions of DNMT1 in breast cancer have been well reviewed. In this article, the roles of DNMT3A and DNMT3B in breast tumorigenesis and development are reviewed. We also discuss the SNP and mutations of DNMT3A and DNMT3B in breast cancer. In addition, we summarize how DNMT3A and DNMT3B are regulated by non-coding RNAs and signaling pathways in breast cancer, and targeting the expression levels of DNMT3A and DNMT3B may be a promising therapeutic approach for breast cancer. This review will provide reference for further studies on the biological functions and molecular mechanisms of DNMT3A and DNMT3B in breast cancer.

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Impact of miRNAs expression modulation on the methylation status of breast cancer stem cell-related genes

Cited by 9 publications

References 20 publications

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

The Role of MicroRNAs Regulated Breast Cancer Stem Cells in the Pathogenesis, Prognosis and Aggressiveness of Breast Cancer

DNMT3A and DNMT3B in Breast Tumorigenesis and Potential Therapy

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