Impact of miR‐26b on cardiomyocyte differentiation in P19 cells through regulating canonical/non‐canonical Wnt signalling

Wang, Duo; Liu, Chang; Wang, Yumei; Wang, Wenjing; Wang, Kang; Wu, Xiujuan; Li, Zhigang; Zhao, Cuimei; Li, Li; Peng, Luying

doi:10.1111/cpr.12371

Cited by 16 publications

(12 citation statements)

References 51 publications

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“…This mode of pairing usually negatively regulates the translation of the target through the repression of the initial ribosome binding to the mRNA or the ribosome drop-off [ 21 ]. The analysis of miRNA expression in cardiomyocyte progenitor cells (CMPCs) showed that 188 miRNAs were detectable in proliferating CMPCs and 195 in differentiated CMPCs such as miR1, miR1-2, miR499, miR322, miR503, miR208, miR133, and miR26b [ 30 , 31 , 32 , 33 , 34 ]. MiR-208, together with miR-1, miR-133, and miR-206, are called myomiRs as they are expressed specifically in the heart and skeletal muscles.…”

Section: Regulatory Pathways and Epigenetic Control Of Cardiomyogementioning

confidence: 99%

Human-Induced Pluripotent Stem Cell Technology and Cardiomyocyte Generation: Progress and Clinical Applications

Baldassarre

Cimetta

Bollini

et al. 2018

Cells

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Human-induced pluripotent stem cells (hiPSCs) are reprogrammed cells that have hallmarks similar to embryonic stem cells including the capacity of self-renewal and differentiation into cardiac myocytes. The improvements in reprogramming and differentiating methods achieved in the past 10 years widened the use of hiPSCs, especially in cardiac research. hiPSC-derived cardiac myocytes (CMs) recapitulate phenotypic differences caused by genetic variations, making them attractive human disease models and useful tools for drug discovery and toxicology testing. In addition, hiPSCs can be used as sources of cells for cardiac regeneration in animal models. Here, we review the advances in the genetic and epigenetic control of cardiomyogenesis that underlies the significant improvement of the induced reprogramming of somatic cells to CMs; the methods used to improve scalability of throughput assays for functional screening and drug testing in vitro; the phenotypic characteristics of hiPSCs-derived CMs and their ability to rescue injured CMs through paracrine effects; we also cover the novel approaches in tissue engineering for hiPSC-derived cardiac tissue generation, and finally, their immunological features and the potential use in biomedical applications.

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Section: Regulatory Pathways and Epigenetic Control Of Cardiomyogementioning

confidence: 99%

Human-Induced Pluripotent Stem Cell Technology and Cardiomyocyte Generation: Progress and Clinical Applications

Baldassarre

Cimetta

Bollini

et al. 2018

Cells

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“…Cells were cultured in α-minimal essential medium with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin and 100 µg/ml streptomycin (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) at 37˚C in an incubator containing 5% CO 2 . To induce P19 cell differentiation, DMSO (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) was added to the culture medium at a final concentration of 1.0% (27)(28)(29). P19 cells aggregated over the following 4 days to form embryoid bodies (EBs), during which time the medium was replenished every 24 h. On day 4, the EBs were transferred into a 6-well plate and cultured in DMSO-free medium to allow adherent proliferation of P19 cells.…”

Section: Methodsmentioning

confidence: 99%

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

Chen

et al. 2018

Int J Mol Med

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Our previous studies identified that the expression of microRNA-29c (miR-29c-3p) was significantly increased in the serum of pregnant women carrying fetuses with congenital heart disease (CHD) compared with in that of normal pregnant women. However, the mechanism by which miR-29c-3p affects development of the embryonic heart remained unclear. The aim of the present study was to investigate the effect and potential molecular mechanism of miR-29c-3p overexpression on P19 cell proliferation, apoptosis and differentiation. miR-29c-3p-overexpression and protein kinase Bγ (Akt3)-knockdown cell lines were constructed using transfection technology. The function of miR-29c-3p and Akt3 in cardiomyocyte development was investigated by determining the proliferation, apoptosis and differentiation of P19 cells, which can differentiate into cardiomyocytes induced by dimethylsulfoxide. Bioinformatic analysis and luciferase assays were performed to explore the association between Akt3 and miR-29c-3p. The results of the present study revealed that miR-29c-3p overexpression and Akt3 knockdown suppressed proliferation, and promoted apoptosis and differentiation in P19 cells. Akt3 was also demonstrated to be a target of miR-29c-3p. Therefore, overexpression of miR-29c-3p may inhibit proliferation, and promote apoptosis and differentiation in P19 cells by inhibiting the expression of Akt3. miR-29c-3p may be a potential therapeutic target for the treatment of CHD.

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“…21 The rest of the information related to the assay is described in Supplemental Experimental Procedures and in the Table S2. 21 The rest of the information related to the assay is described in Supplemental Experimental Procedures and in the Table S2.…”

Section: Immunostainingmentioning

confidence: 99%

“…The major experimental steps are described in accordance with the previous description. 21 The rest of the information related to the assay is described in Supplemental Experimental Procedures and in the…”

Section: Immunostainingmentioning

confidence: 99%

Laminin promotes differentiation of rat embryonic stem cells into cardiomyocytes by activating the integrin/FAK/PI3K p85 pathway

Wang

Liu

et al. 2019

J Cellular Molecular Medi

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The generation of germline competent rat embryonic stem cells (rESCs) allows the study of their lineage commitment. Here, we developed a highly efficient system for rESC‐derived cardiomyocytes, and even the formation of three‐dimensional (3D)‐like cell clusters with cTNT and α‐Actinin. We have validated that laminin can interact with membrane integrin to promote the phosphorylation of both phosphatidylinositol 3‐kinase (PI3K) p85 and the focal adhesion kinase (FAK). In parallel, GATA4 was up‐regulated. Upon inhibiting the integrin, laminin loses the effect on cardiomyocyte differentiation, accompanied with a down‐regulation of phosphorylation level of PI3K p85 and FAK. Meanwhile, the expression of Gata4 was inhibited as well. Taken together, laminin is a crucial component in the differentiation of rESCs into cardiomyocytes through increasing their proliferation via interacting with integrin pathway. These results provide new insights into the pathways mediated by extracellular laminin involved in the fate of rESC‐derived cardiomyocytes.

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Impact of miR‐26b on cardiomyocyte differentiation in P19 cells through regulating canonical/non‐canonical Wnt signalling

Abstract: Our results indicated that miR-26b exerts a role on promoting cardiomyocyte differentiation of P19 cells by controlling the canonical and non-canonical Wnt signalling.

Cited by 16 publications

References 51 publications

Human-Induced Pluripotent Stem Cell Technology and Cardiomyocyte Generation: Progress and Clinical Applications

Human-Induced Pluripotent Stem Cell Technology and Cardiomyocyte Generation: Progress and Clinical Applications

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

Laminin promotes differentiation of rat embryonic stem cells into cardiomyocytes by activating the integrin/FAK/PI3K p85 pathway

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