Impact of microstructure on cell behavior and tissue mechanics in collagen and dermal decellularized extra-cellular matrices

Girardeau‐Hubert, Sarah; Lynch, Barbara; Zuttion, Francesca; Label, Rabab; Rayee, Chrystelle; Brizion, Sébastien; Ricois, Sylvie; Martinez, Anthony; Park, Eunhye; Kim, Chang-Hwan; Marinho, Paulo A.; Shim, Jin‐Hyung; Jin, Songwan; Rielland, Maïté; Sœur, Jérémie

doi:10.1016/j.actbio.2022.02.035

Cited by 15 publications

(21 citation statements)

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“…The digestion process for dECM is typically a combination of an acid with pepsin. The most common acid used is HCl at a concentration of 0.01 M [ 14 , 56 , 82 , 86 , 87 , 90 , 92 , 109 , 111 , 117 , 138 , 143 , 163 , 168 , 171 , 172 , 173 , 187 , 195 , 197 , 199 , 200 , 202 , 203 , 211 , 227 , 237 , 321 , 324 ], though acetic acid at a concentration of 0.5 M is another popular acid [ 83 , 96 , 107 , 114 , 115 , 125 , 136 , 139 , 159 , 160 , 161 , 162 , 201 , 320 ], with both used at a ratio of 1 g of dECM powder:100 mg pepsin:100 mL acid and stirred for 48 h. There are different effects of the type and concentration of acid used, with 0.1 M HCl accelerating the digestion, creating higher cell viability, migration, and proliferation, and enhancing the ability to induce tissue formation, though it results in a softer and more unstable gel than acetic acid [ 325 ]. There are also optional steps after solubilizing dECM; the first is to filter the colloidal dispersion to remove the undigested particles that might affect later use [ 125 , 159 , ...…”

Section: Ecm Modification and Methodsmentioning

confidence: 99%

“…Kidney dECM has been shown to be more effective at promoting the recruitment and migration of host cells, accelerate the formation of vascular networks and maintain vascular integrity, and contribute to the self-arrangement and maturation of cells to form glomerular-like structures in vivo [ 98 ]. Fibroblasts have been shown to be more responsive to their microenvironment when grown in skin dECM bioink compared to type I collagen [ 107 ]. Cartilage dECM has been shown to be effective at preventing chondrocyte hypertrophy and calcification of the cartilage in the repair of cartilage defects [ 108 ].…”

Section: Structure and Properties Of Ecmmentioning

confidence: 99%

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Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.

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Section: Ecm Modification and Methodsmentioning

confidence: 99%

Section: Structure and Properties Of Ecmmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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“…Therefore, it can only be used as a temporary dressing. 3D printing technology can help researchers to precisely manufacture materials based on optimally designed structures and active ingredient formula composition, enabling the creation of artificial substances that are more flexible and tailored for accurate therapeutic purposes. , Girardeau-Hubert et al demonstrated in vitro that 3D-printed decellularized ECM (dECM)-based skin analogues can promote the response of epidermal-forming cells and fibroblasts to the microenvironment compared with type I collagen-based skin analogues. Chen et al used 3D printing to prepare dermal analogues with pore sizes similar to those of the human dermal matrix, which significantly improved the appearance of healing and reduced scar contraction in vivo compared with commercial dermal matrix materials (Pelnac) and split-thickness skin graft (STSG).…”

Section: Introductionmentioning

confidence: 99%

Copper-Epigallocatechin Gallate Enhances Therapeutic Effects of 3D-Printed Dermal Scaffolds in Mitigating Diabetic Wound Scarring

Xiong

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

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Morbid dermal templates, microangiopathy, and abnormal inflammation are the three most critical reasons for the scarred healing and the high recurrence rate of diabetic wounds. In this present study, a combination of a methacrylated decellularized extracellular matrix (ECMMA, aka EM)-based hydrogel system loaded with copperepigallocatechin gallate (Cu-EGCG) capsules is proposed to fabricate bio-printed dermal scaffolds for diabetic wound treatment. Copper ions act as a bioactive element for promoting angiogenesis, and EGCG can inhibit inflammation on the wound site. In addition to the above activities, EM/Cu-EGCG (E/C) dermal scaffolds can also provide optimized templates and nutrient exchange space for guiding the orderly deposition and remodeling of ECM. In vitro experiments have shown that the E/C hydrogel can promote angiogenesis and inhibit the polarization of macrophages to the M1 proinflammatory phenotype. In the full-thickness skin defect model of diabetic rats, the E/C dermal scaffold combined with split-thickness skin graft transplantation can alleviate pathological scarring via promoting angiogenesis and driving macrophage polarization to the anti-inflammatory M2 phenotype. These may be attributed to the scaffold-actuated expression of angiogenesis-related genes in the HIF-1α/vascular endothelial growth factor pathway and decreased expression of inflammation-related genes in the TNF-α/NF-κB/MMP9 pathway. The results of this study show that the E/C dermal scaffold could serve as a promising artificial dermal analogue for solving the problems of delayed wound healing and reulceration of diabetic wounds.

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“…In the past few decades, dECM has attracted great research interest due to its good biocompatibility, biological activity, and moderate mechanical properties. Various bioscaffolds derived from dECM have been widely used in tissue engineering, such as dermal tissue repair, heart valve replacement, and vascular tissue regeneration (27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

Biomimetic anisotropic macroporous cryogel promotes the repair of osteoporotic bone defects through altering the ROS niche via down-regulating the ROMO1

Hai

Pan

Liu

et al. 2023

Preprint

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Background Osteoporosis is a systemic bone disease prone to fractures due to decreased bone density and bone quality. The pathological environment of osteoporosis interferes with the normal process of fracture healing. The clinical regenerative repair materials specifically used for osteoporotic bone defects are not satisfactory, and the study of the mechanism of bone defect regeneration in the pathological environment of osteoporosis is helpful for the design of related materials.Methods In this study, the cryogels were prepared from decellularized extracellular matrix (dECM), methacrylate gelatin (GelMA), and carboxymethyl chitosan (CMCS) via unidirectional freezing, photocrosslinking, and genipin crosslinking. dECM extracted from normal or osteoporotic rats was applied for the preparation of the cryogels, named as GelMA-CMCS@Normal or GelMA-CMCS@OVX, respectively. The effects of different cryogels on BMSCs isolated from osteoporotic rats (OVX-BMSCs) were observed in vitro and in vivo.Results It was verified that the cryogels had excellent in vitro and in vivo biocompatibility. Furthermore, the GelMA-CMCS@Normal could effectively improve the proliferation of OVX-BMSCs, and promote the differentiation of OVX-BMSCs into osteoblasts in vitro and in vivo. RNA sequencing found that the OVX-BMSCs co-cultured with GelMA-CMCS@Normal cryogel exhibited down-regulated expression of reactive oxygen species modulator 1 (Romo1), which could activate the expression of nuclear factor erythroid 2-related factor 2 (Nfe2l2, NRF2). Further evidence showed that the reactive oxygen species (ROS) of the OVX-BMSCs were scavenged effectively after co-cultured with the GelMA-CMCS@Normal cryogel.Conclusions The results indicated that GelMA-CMCS@Normal cryogel was expected to be a clinical candidate for the repair of osteoporotic bone defects by regulating the ROS niche of OVX-BMSCs.Trial registration: Not applicable.

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Impact of microstructure on cell behavior and tissue mechanics in collagen and dermal decellularized extra-cellular matrices

Cited by 15 publications

References 50 publications

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Copper-Epigallocatechin Gallate Enhances Therapeutic Effects of 3D-Printed Dermal Scaffolds in Mitigating Diabetic Wound Scarring

Biomimetic anisotropic macroporous cryogel promotes the repair of osteoporotic bone defects through altering the ROS niche via down-regulating the ROMO1

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