Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study

Mitala, Christina M.; Wang, Yuexiang; Borland, Laura M.; Jung, Moon Chul; Shand, Stuart H.; Watkins, Simon C.; Weber, Stephen G.; Michael, Adrian C.

doi:10.1016/j.jneumeth.2008.06.034

Cited by 61 publications

(80 citation statements)

References 50 publications

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“…8. 37 This is evidence that the probes open the BBB. We also found that fluorescent nanobeads used to represent blood flow spill out of blood vessels near microdialysis probes, but not elsewhere in the brain, providing direct evidence that the BBB is opened, as shown in Fig.…”

Section: Immunohistology Of Probe Implantation and Mitigating Damagementioning

confidence: 94%

A review of the effects of FSCV and microdialysis measurements on dopamine release in the surrounding tissue

Jaquins-Gerstl

Michael

2015

Analyst

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Microdialysis is commonly used in neuroscience to obtain information about the concentration of substances, including neurotransmitters such as dopamine (DA), in the extracellular space (ECS) of the brain. Measuring DA concentrations in the ECS with in vivo microdialysis and/or voltammetry is a mainstay of investigations into both normal and pathological function of central DA systems. Although both techniques are instrumental in understanding brain chemistry each has its shortcomings. The objective of this review is to characterize some of the tissue and DA differences associated with each technique in vivo. Much of this work will focus on immunohistochemical and microelectrode measurements of DA in the tissue next to the microdialysis probe and mitigating the response to the damage caused by probe implantation.

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Section: Immunohistology Of Probe Implantation and Mitigating Damagementioning

confidence: 94%

A review of the effects of FSCV and microdialysis measurements on dopamine release in the surrounding tissue

Jaquins-Gerstl

Michael

2015

Analyst

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“…The procedures for immunohistochemistry and fluorescence microscopy are described in our previous papers. 32,[48][49] Rats were deeply anesthetized with isoflurane (2.5% by volume O 2 ) and perfused transcardially with 200 ml 0.01 M phosphate-buffered saline (PBS), pH 7.4, followed by 160mL of 4% paraformaldehyde, and then with 50mL of a solution containing commercially available Table 1. The noise in a 10-min baseline prior to each needle prick was used to create a threshold for detection of the glucose dip (see Figure S4 for details).…”

Section: All Procedures Involving Animals Were Approved By the Univermentioning

confidence: 99%

Enhancing Continuous Online Microdialysis Using Dexamethasone: Measurement of Dynamic Neurometabolic Changes during Spreading Depolarization

Varner

Leong

Jaquins-Gerstl

et al. 2017

ACS Chem. Neurosci.

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Microdialysis is well established in chemical neuroscience as a mainstay technology for real time intracranial chemical monitoring in both animal models and human patients. The capabilities of microdialysis sampling have the potential to be further enhanced through mitigation of the penetration injury unavoidably caused by the insertion of microdialysis probes into brain tissue. Herein, we show that dexamethasone retrodialysis in the rat cortex enhances the microdialysis detection of K+ and glucose transients induced by spreading depolarization. Once inserted, the probes were perfused continuously (1.67 μL/min) either with or without dexamethasone. Without dexamethasone, glucose transients were too small to reliably quantify at 5 days after probe insertion. With dexamethasone, robust K+ and glucose transients were readily quantified at 2 hrs, 5 days, and 10 days after probe insertion. Although the amplitudes of the K+ transients declined day-to-day following probe insertion, amplitudes of the glucose transients were consistent. Immunohistochemistry and fluorescence microscopy confirm that dexamethasone is highly effective at preventing ischemia and gliosis in the vicinity of microdialysis probes implanted for 10 days.

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“…The results indicate confusion from tissue damage. (59,60) How can we measure in spaces of nanometer dimensions without destroying the very architecture that determines the problem of interest? Below we present two approaches, both with strengths and weaknesses.…”

Section: Measuring In Vivo Profilesmentioning

confidence: 99%

Windows to cell function and dysfunction: Signatures written in the boundary layers

Smith¹,

Collis²,

Messerli³

2010

BioEssays

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The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments whose profiles both reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily with cultured cells but we extend our consideration to the far more complex environment of tissues and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task 20 in hand. IntroductionThe advent of electron tomography with 3 dimensional image reconstruction has revealed a fundamental beauty and complexity behind the structure of the living cell. Notable contributions have come from several imaging laboratories but one of the most compelling can be found in Marsh et al.(1) From such reconstructions the complexity within a cell is quite staggering and in the 4 th dimension of time this entire structure is in motion. The question that becomes extremely interesting is how molecular and ionic signals are regulated and move within these matrices, where chemical diffusion will be significantly altered. (2) However, complexity, both structural and functional, does not stop at the plasma membrane but extends outwards 30 into the extracellular/intercellular space (EICS: Fig.1). The chemical dynamics within this space are hypothesized to play key roles in cancer, spreading depression, epilepsy and sleep disorders. (3)(4)(5)(6) The purpose of this essay is to consider what we can learn from this space and the methods for following the dynamics of chemical movement within the diffusive boundary layers that comprise an integral part of a cell. We will start by noting the physical and chemical features involved.

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Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study

Cited by 61 publications

References 50 publications

A review of the effects of FSCV and microdialysis measurements on dopamine release in the surrounding tissue

A review of the effects of FSCV and microdialysis measurements on dopamine release in the surrounding tissue

Enhancing Continuous Online Microdialysis Using Dexamethasone: Measurement of Dynamic Neurometabolic Changes during Spreading Depolarization

Windows to cell function and dysfunction: Signatures written in the boundary layers

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