Impact of mesenchymal stem cell-secretome-loaded hydrogel on proliferative and migratory activities of hyperglycemic fibroblasts

Sears, Victoria; Danaoui, Youssef; Ghosh, Gargi

doi:10.1016/j.mtcomm.2021.102285

Cited by 11 publications

(12 citation statements)

References 38 publications

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“…The wound healing of DFU requires the collaboration of multiple types of cells. MSC-Exos also target fibroblasts and keratinocytes for the acceleration of re-epithelialization, collagen deposition, and remodeling via regulating MAPK/ERK ( Li et al, 2016 ), PI3K/AKT ( Li et al, 2015 ; Zhang et al, 2018 ; Sears et al, 2021 ), and Wnt/β-catenin ( Lv et al, 2020 ) pathways. Engineered hADSC-Exos overexpressing miR-21 significantly strengthened the migration and proliferation of keratinocytes by upregulating Matrix Metallopeptidase 7 ( Lv et al, 2020 ).…”

Section: Msc-exos For Therapeutic Applications In Microbial Diseasesmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosome Therapy of Microbial Diseases: From Bench to Bed

Wu¹,

Jin²,

Ding³

et al. 2022

Front. Microbiol.

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Microbial diseases are a global health threat, leading to tremendous casualties and economic losses. The strategy to treat microbial diseases falls into two broad categories: pathogen-directed therapy (PDT) and host-directed therapy (HDT). As the typical PDT, antibiotics or antiviral drugs directly attack bacteria or viruses through discerning specific molecules. However, drug abuse could result in antimicrobial resistance and increase infectious disease morbidity. Recently, the exosome therapy, as a HDT, has attracted extensive attentions for its potential in limiting infectious complications and targeted drug delivery. Mesenchymal stem cell-derived exosomes (MSC-Exos) are the most broadly investigated. In this review, we mainly focus on the development and recent advances of the application of MSC-Exos on microbial diseases. The review starts with the difficulties and current strategies in antimicrobial treatments, followed by a comprehensive overview of exosomes in aspect of isolation, identification, contents, and applications. Then, the underlying mechanisms of the MSC-Exo therapy in microbial diseases are discussed in depth, mainly including immunomodulation, repression of excessive inflammation, and promotion of tissue regeneration. In addition, we highlight the latest progress in the clinical translation of the MSC-Exo therapy, by summarizing related clinical trials, routes of administration, and exosome modifications. This review will provide fundamental insights and future perspectives on MSC-Exo therapy in microbial diseases from bench to bedside.

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Section: Msc-exos For Therapeutic Applications In Microbial Diseasesmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosome Therapy of Microbial Diseases: From Bench to Bed

Wu¹,

Jin²,

Ding³

et al. 2022

Front. Microbiol.

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“…A recent study developed by Victoria et al focused on developing mesenchymal stem cell (MSC)-conditioned media (CM) loaded in hydrogel and its application in an in vitro hyperglycemic human dermal fibroblast to investigate the wound healing potential [ 92 ]. The components of the hydrogel were GelMA-PEGDA, loaded with MSC-CM, which demonstrated higher proliferation of the hyperglycemic fibroblast due to the combined effects of matrix properties together with the prolonged release of MSC-secreted bioactive molecules.…”

Section: Structural Formulation Using Biomaterials With Secretome For...mentioning

confidence: 99%

“…The components of the hydrogel were GelMA-PEGDA, loaded with MSC-CM, which demonstrated higher proliferation of the hyperglycemic fibroblast due to the combined effects of matrix properties together with the prolonged release of MSC-secreted bioactive molecules. Hence, it was potentially beneficial in diabetic chronic wounds [ 92 ].…”

Section: Structural Formulation Using Biomaterials With Secretome For...mentioning

confidence: 99%

Cell Secretome Strategies for Controlled Drug Delivery and Wound-Healing Applications

et al. 2022

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There is significant interest in using stem cells in the management of cutaneous wounds. However, potential safety, efficacy, and cost problems associated with whole-cell transplantation hinder their clinical application. Secretome, a collective of mesenchymal stem-cell-stored paracrine factors, and immunomodulatory cytokines offer therapeutic potential as a cell-free therapy for the treatment of cutaneous wounds. This review explores the possibility of secretome as a treatment for cutaneous wounds and tissue regeneration. The review mainly focuses on in vitro and in vivo investigations that use biomaterials and secretome together to treat wounds, extend secretome retention, and control release to preserve their biological function. The approaches employed for the fabrication of biomaterials with condition media or extracellular vesicles are discussed to identify their future clinical application in wound treatment.

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“…There is thus a disadvantage in topical delivery of the secretome without a vehicle, and consequently a growing interest in sustained delivery of the secretome to wounds. A majority of research groups working on sustained delivery of the secretome have employed hydrogel systems as a delivery vehicle [24][25][26][27][28] due to the advantages of hydrogels to adsorb or encapsulate drugs. [29,30] Hydrogels are also popular as wound healing biomaterials due to their high water content, low elastic modulus, and generally porous structure.…”

Section: Introductionmentioning

confidence: 99%

Squid Suckerin‐Spider Silk Fusion Protein Hydrogel for Delivery of Mesenchymal Stem Cell Secretome to Chronic Wounds

Koh

Wang

Yuan

et al. 2022

Adv Healthcare Materials

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Chronic wounds are non‐healing wounds characterized by a prolonged inflammation phase. Excessive inflammation leads to elevated protease levels and consequently to a decrease in growth factors at wound sites. Stem cell secretome therapy has been identified as a treatment strategy to modulate the microenvironment of chronic wounds via supplementation with anti‐inflammatory/growth factors. However, there is a need to develop better secretome delivery systems that are able to encapsulate the secretome without denaturation, in a sustained manner, and that are fully biocompatible. To address this gap, a recombinant squid suckerin‐spider silk fusion protein is developed with cell‐adhesion motifs capable of thermal gelation at physiological temperatures to form hydrogels for encapsulation and subsequent release of the stem cell secretome. Freeze–thaw treatment of the protein hydrogel results in a modified porous cryogel that maintains slow degradation and sustained secretome release. Chronic wounds of diabetic mice treated with the secretome‐laden cryogel display increased wound closure, presence of endothelial cells, granulation wound tissue thickness, and reduced inflammation with no fibrotic scar formation. Overall, these in vivo indicators of wound healing demonstrate that the fusion protein hydrogel displays remarkable potential as a delivery system for secretome‐assisted chronic wound healing.

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Impact of mesenchymal stem cell-secretome-loaded hydrogel on proliferative and migratory activities of hyperglycemic fibroblasts

Cited by 11 publications

References 38 publications

Mesenchymal Stem Cell-Derived Exosome Therapy of Microbial Diseases: From Bench to Bed

Mesenchymal Stem Cell-Derived Exosome Therapy of Microbial Diseases: From Bench to Bed

Cell Secretome Strategies for Controlled Drug Delivery and Wound-Healing Applications

Squid Suckerin‐Spider Silk Fusion Protein Hydrogel for Delivery of Mesenchymal Stem Cell Secretome to Chronic Wounds

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