“…Besides, to account for the wide diversity of PUFAs, variations of those membrane properties were investigated upon treatment with PUFAs and PUFA-PLs harboring different number of double bonds and differing in the location of their first double bond (3 and 6). Importantly, previous studies [42] have demonstrated that the incubation of cells with PUFAs allows for their incorporation into cellular membranes, mostly in PC and PE, with a 5-10% enrichment level closely resembling the deficiency levels observed in patients suffering from psychiatric disorders [67]. By means of Raman imaging, we here further revealed that PUFA only accumulate in the plasma membrane, rather than in other intracellular organelles membrane.…”