Impact of Melatonin and Molecular Clockwork Components on the Expression of Thyrotropin Beta Chain (Tshb) and the Tsh Receptor in the Mouse Pars Tuberalis

Unfried, Claudia; Ansari, Nariman; Yasuo, Shinobu; Korf, Horst‐Werner; Gall, Charlotte von

doi:10.1210/jcem.94.9.9999

Cited by 17 publications

(43 citation statements)

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“…Another possibility may be that the melatonin signal affecting the POMC system and thus the Pomc expression in the ARC and PD is decoded in the hypophysial pars tuberalis which contains a high density of MT1 receptors [8] and conveys the MT1-mediated melatonin signal retrogradely via thyrotropin to the hypothalamus including the ARC and anterogradely via endocannabinoids to the PD [14,32,[49][50][51][52] .…”

Section: Discussionmentioning

confidence: 99%

“…F6/19) reported here were conducted in accordance with accepted standards of humane animal care and were consistent with Federal Guidelines and Directive of the European Union (2010/63/EU). Mice with a targeted deletion of the MT1 gene (MT1-/-) and the corresponding WT were bred onto melatonin-proficient C3H/HeN background for at least 10 generations [14,32,33] . Experiments were performed with adult (10-to 18-week-old) male littermates obtained by heterozygous breeding.…”

Section: Animalsmentioning

confidence: 99%

“…Melatonin injections were performed according to the protocol described in Unfried et al [32] . WT mice were subcutaneously injected once either with 10 μg melatonin (Sigma-Aldrich, Schnellendorf, Germany) dissolved in 100 μl of 5% ethanol and 0.9% NaCl or with vehicle in the middle of the subjective day (CT6).…”

Section: Melatonin Injectionmentioning

confidence: 99%

“…Radioactive in situ hybridization was carried out with brain and pituitary cryosections of WT and MT1-/-mice (4 mice/genotype and time point) as previously described [32] . The following antisense or the corresponding sense 45-mer probes for Pomc and Npy mRNA expression were used ( Pomc sense: 5 ′ -CCA GAG CCG AGT CCA CGC GAG GGC AAG GCG TCC TAC TCC ATG GAG-3 ′ ; Pomc antisense: 5 ′ -CTC CAT GGA GTA GGA GCG CTT GCC CTC GCG TGG ACT CGG CTC TGG-3 ′ ; Npy sense: 5 ′ -GGG TGT CCC ACC AAT GCA TGC CAC CAC TAG GCT GGA CTC CGC CCC-3 ′ , Npy antisense: 5 ′ -GGG GCG GAG TCC AGC CTA GTG GTG GCA TGC ATT GGT GGG ACA GGC-3 ′ ).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

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Melatonin Receptor 1 Deficiency Affects Feeding Dynamics and Pro-Opiomelanocortin Expression in the Arcuate Nucleus and Pituitary of Mice

Fischer¹,

Mueller²,

Pfeffer³

et al. 2016

Neuroendocrinology

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Background/Methods: Melatonin, the neurohormone for darkness, mediates photoperiod-dependent changes in physiology and behavior by targeting specific membrane-bound receptors (MT1 and MT2). In the present study, we investigated the impact of MT1 receptor deficiency on feeding behavior, locomotor activity and mRNA expression levels encoding for the polypeptide pro-opiomelanocortin (Pomc) and neuropeptide Y (Npy) in the hypothalamic arcuate nucleus (ARC) and the adenohypophysis [pars distalis (PD) and pars intermedia (PI)] in a comparison between wild-type (WT) and MT1-deficient (MT1-/-) mice. Results: The MT1-/- mice spent significantly more time feeding than the WT mice, while the general locomotor behavior, body weight and the total amount of food consumed did not differ between both genotypes. The nocturnal expression levels of Pomc in the ARC and PD were significantly higher in WT as compared to MT1-/- mice and exogenous melatonin administered during the light phase stimulated Pomc expression in WT mice only. No differences were found between WT and MT1-/- mice with regard to Pomc expression levels in the PI. Conclusion: Thus, the MT1-mediated signaling stimulates Pomc expression in a region-specific pattern. Since the MT1-mediated changes in Pomc expression do not elicit direct orexigenic or anorexigenic effects, such effects are obviously mediated by regulatory systems downstream of the Pomc mRNA (e.g. cleavage and release of POMC derivatives), which are independent of MT1 signaling.

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Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Melatonin Injectionmentioning

confidence: 99%

Section: In Situ Hybridizationmentioning

confidence: 99%

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Melatonin Receptor 1 Deficiency Affects Feeding Dynamics and Pro-Opiomelanocortin Expression in the Arcuate Nucleus and Pituitary of Mice

Fischer¹,

Mueller²,

Pfeffer³

et al. 2016

Neuroendocrinology

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“…Recently, thyrotropin (TSH), whose expression in the PT was first described two decades ago (Wittkowski et al 1988;Böckers et al 1990), has been identified as a retrograde messenger from the PT: the expression of thyrotropin-beta (TSH-β) is rapidly induced in the PT of Japanese quail exposed to long-day conditions, and PTderived TSH acts on the ependymal cell layer of the infundibular recess of the third ventricle to regulate several genes including type 2 and type 3 deiodinases (Nakao et al 2008). In sheep and mice, PT-derived TSH appears to play a similar role (Hanon et al 2008;Ono et al 2008;Unfried et al 2009). Notably, the molecules conveying "anterograde" signals from the PT to the PD have not yet been identified.…”

Section: Introductionmentioning

confidence: 99%

An endocannabinoid system is localized to the hypophysial pars tuberalis of Syrian hamsters and responds to photoperiodic changes

et al. 2010

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The hypophysial pars tuberalis (PT), an important interface between neuroendocrine brain centers (hypothalamus, pineal organ) and the pars distalis (PD) of the hypophysis, plays a central role in regulating seasonal reproduction and prolactin release. However, the signaling molecules that transmit photoperiodic information from the PT to the PD and control prolactin release (the so-called "tuberalins") have not yet been identified, despite an intense search for more than three decades. Here, we demonstrate an endocannabinoid system in the PT of the Syrian hamster, a photoperiodic species. By means of in situ hybrization, the PT was found to express N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD), fatty acid amide hydrolase (FAAH), sn-1-selective diacylglycerol lipases (DAGLalpha and DAGLbeta), and monoacylglycerol lipase (MAGL), enzymes involved in endocannabinoid synthesis and degradation. The expression of NAPE-PLD, FAAH, and DAGLalpha was confirmed by immunohistochemistry. Expression and protein levels of DAGLs controlling the synthesis of 2-arachidonoyl glycerol (2-AG), a major endocannabinoid, were upregulated in the PT of Syrian hamsters kept under long-day conditions. Consequently, 2-AG levels were increased in the PT of these hamsters. A primary target of 2-AG, the cannabinoid receptor 1 (CB1), was expressed in the PD. Double-immunolabeling revealed that most of the CB1-immunoreactive cells in the PD were folliculostellate cells that were also immunoreactive for S-100 protein. Thus, the PT comprises an endocannabinoid system, and 2-AG may act as a photoperiodic messenger from the PT to the PD for the regulation of hypophysial hormonal secretion.

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Comparative transcriptomics of the Djungarian hamster hypothalamus during short photoperiod acclimation and spontaneous torpor

Haugg¹,

Borner

Diedrich³

et al. 2021

FEBS Open Bio

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Djungarian hamsters (Phodopus sungorus, Cricetidae) are photoperiodic mammals [1,2]. In summer or summer-like long photoperiod (LP; e.g., 16:8 light:darkness), they have a high body mass, body temperature, and activity level. They are reproductively active and camouflaged to their environment by a light Abbreviations HT, hypothermia; log2(FC), logarithm of fold change to the base 2; LP, summer-like long photoperiod; NT, normothermia; padj, adjusted ?P-value; SP, winter-like short photoperiod; Tb, core body temperature; ZT, Zeitgeber-time.

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Impact of Melatonin and Molecular Clockwork Components on the Expression of Thyrotropin Beta Chain (Tshb) and the Tsh Receptor in the Mouse Pars Tuberalis

Cited by 17 publications

References 0 publications

Melatonin Receptor 1 Deficiency Affects Feeding Dynamics and Pro-Opiomelanocortin<b> </b>Expression in the Arcuate Nucleus and Pituitary of Mice

Melatonin Receptor 1 Deficiency Affects Feeding Dynamics and Pro-Opiomelanocortin<b> </b>Expression in the Arcuate Nucleus and Pituitary of Mice

An endocannabinoid system is localized to the hypophysial pars tuberalis of Syrian hamsters and responds to photoperiodic changes

Comparative transcriptomics of the Djungarian hamster hypothalamus during short photoperiod acclimation and spontaneous torpor

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