Impact of magnesium ions on lysozyme-triggered disintegration and solubilization of waste activated sludge

Zhang, Pengfei; He, Junguo; Zou, Xiang; Zhong, Yijie; Pan, Xinlei; Zhang, Jie; Pang, Heliang

doi:10.1016/j.jenvman.2022.115148

Cited by 13 publications

(3 citation statements)

References 49 publications

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“…Asim et al showed that abundant hydroxyl and carboxyl groups in carboxymethylcellulose (CMC) hydrogel extractants together improved the affinity toward certain metal ions in aqueous samples . Lysozyme from chicken egg white was used as a natural nitrogen and oxygen source and provided a structure-directing effect in the formation of the carbon material . The fabricated carbo-gel was rich in oxygen and carbon moieties and was cast as a thin film using the chitosan biopolymer to avoid adsorbent leaching into the samples.…”

Section: Introductionmentioning

confidence: 99%

“…8 Lysozyme from chicken egg white was used as a natural nitrogen and oxygen source and provided a structuredirecting effect in the formation of the carbon material. 9 The fabricated carbo-gel was rich in oxygen and carbon moieties and was cast as a thin film using the chitosan biopolymer to avoid adsorbent leaching into the samples. The free amino groups present in the chitosan contribute to the properties including polycationic, chelating, and film-forming as well as ready solubility in dilute acetic acid, which has increased the significance of the resulting sorbent in terms of ample active sites.…”

Section: ■ Introductionmentioning

confidence: 99%

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Lysozyme Carbo-Gel Integrated Repackable Miniaturized Microextraction Device: A Platform for Rapid Enrichment and Sensitive Detection of Multimetal Ions

Singh,

Arputharaj,

et al. 2023

ACS EST Eng.

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Sensing and identifying toxic materials within a complex matrix require development of sensitive and selective analytical devices that can enrich the targeted chemical for analysis. We developed a simple and easy approach to enrich and detect various toxic metal ions from different sample matrices by using a microextraction platform device in combination with inductively coupled plasma mass spectrometry. We fabricated a well-defined carbo-gel nanosphere extractant by combining carboxymethylcellulose as a core template with lysozyme, a structural-directing compact globular protein, using hydrothermal carbonization. This was packed into a 3D-printed miniaturized microextraction device that could be repacked and reused multiple times. The surface chemistry and structural significance of the fabricated extractant were studied using electron microscopy and spectroscopy as well as infrared spectroscopy technologies. The detection limits of the established method for Pb 2+ , Bi 3+ , Ag + , Cd 2+ , As 3+ , and Co 2+ ions were in the range of 0.5−125 ng mL −1 , and the relative standard deviations ranged from 2.1 to 4.6%. High recovery between 90.0 and 103% was observed in complex matrices including real and spiked urine samples and was maintained after six regeneration cycles, where the extractant recovery remained above 85%. The multiplex approach of both extractant and device development can significantly improve selective sensing of toxic or emerging contaminants of concern in complex solutions, which demonstrates its major analytical advancement.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Lysozyme Carbo-Gel Integrated Repackable Miniaturized Microextraction Device: A Platform for Rapid Enrichment and Sensitive Detection of Multimetal Ions

Singh,

Arputharaj,

et al. 2023

ACS EST Eng.

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“…Chemical methods include acidolysis, oxidation treatment, 13 and alkaline treatment. 14 The biological methods involve introducing bacteria capable of secreting extracellular enzymes into the sludge, or employing direct addition of enzyme preparations like lysozyme 15,16 or adding biological surfactants with special physical and chemical properties. Among them, thermal and alkali treatment are currently widely utilized.…”

mentioning

confidence: 99%

Study on the effect of alkali on the production of volatile fatty acids from rhamnolipid‐alkali‐thermal pretreated waste activated sludge

Chuanchuan,

Yuling,

Shuaishuai

et al. 2024

Env Prog and Sustain Energy

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This article systematically explores the strengthening effect of NaOH dosage on the fermentation acid production of Waste Activated Sludge (WAS) by the rhamnolipids‐alkali‐thermal combined pretreatment. Analyzed the dissolution characteristics of organic matter in sludge, changes in total volatile fatty acids (VFAs) content and components, and microbial community structure. Research has shown that increasing the amount of NaOH can promote the dissolution of sludge extracellular polymeric substances (EPS) and cell lysis. When NaOH dose was 60 mg/g VS, the dissolution of SCOD was the largest, reaching 5427.78 mg/L. It provides high‐quality substrate for anaerobic flora through the decomposition of refractory humus‐like substances and macromolecular substances, thus improving the production of VFAs. When NaOH dose was 80 mg/g VS, the maximum VFAs yield was 1663.76 mg C/L, and the fermentation type was butyric acid. The increase of NaOH dose promotes the effective enrichment of bacteria, and the dominant bacteria are Proteobacteria, Firmicutes, and Bacteroides at the gate level, with different abundance. The fermentation time plays a decisive role in the evolution of microbial population, and the increase of NaOH dose promotes the enrichment of acid‐producing bacteria, thus improving the production of VFAs.

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Novel nitrogen-doped biochar supported magnetite promotes anaerobic digestion: Material characterization and metagenomic analysis

Zhong

Zhang

et al. 2023

Bioresource Technology

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Impact of magnesium ions on lysozyme-triggered disintegration and solubilization of waste activated sludge

Cited by 13 publications

References 49 publications

Lysozyme Carbo-Gel Integrated Repackable Miniaturized Microextraction Device: A Platform for Rapid Enrichment and Sensitive Detection of Multimetal Ions

Lysozyme Carbo-Gel Integrated Repackable Miniaturized Microextraction Device: A Platform for Rapid Enrichment and Sensitive Detection of Multimetal Ions

Study on the effect of alkali on the production of volatile fatty acids from rhamnolipid‐alkali‐thermal pretreated waste activated sludge

Novel nitrogen-doped biochar supported magnetite promotes anaerobic digestion: Material characterization and metagenomic analysis

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