Impact of macroporous silica nanoparticles at sub-50nm on bio-behaviors and biosafety in drug-resistant cancer models

Xu, Weixia; Zhou, Min; Guo, Zhihan; Lin, Sijin; Li, Mingyu; Kang, Qi; Xu, Yang; Zhang, Xiaokun; Xie, Jingjing

doi:10.1016/j.colsurfb.2021.111912

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…Specifically stated, HeLa-Bcl2 and HepG2-Bcl2-GFP cell lines were, respectively, constructed by our and Dr. Wu’s labs as previously reported . The drug resistance characters of HepG2-Bcl2-GFP, Hela-Bcl2, and LS174T cell lines were previously confirmed by our reports. ,, …”

Section: Methodssupporting

confidence: 56%

“…8 The drug resistance characters of HepG2-Bcl2-GFP, Hela-Bcl2, and LS174T cell lines were previously confirmed by our reports. 8,20,21 2.6. Intracellular pH-Responsive Drug Release Studies.…”

Section: In Vitromentioning

confidence: 99%

“…HeLa-Bcl2 and LS174T cells were seeded in 96-well culture plates at the density of 3 × 10 3 / well and then incubated overnight. The medium was replaced with various drug formulations (N9, Ce, Ce + N9, N9@ M-CA∼8P, N9@ M-CA∼8P + Ce) in complete media at gradient nanoparticle concentrations (1,10,20,50, 100, 200 μg/mL) or at the given Ce concentrations (0.15, 0.3, 0.5, 0.8 μg/mL). Untreated cells represented a negative control with 100% viability.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

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Acid-Responsive Macroporous Silica Nanoparticles for Bcl-2-Functional-Converting Peptide Release and Synergism with Celastrol for Enhanced Therapy against Resistant Cancer

Zhou

Lin

et al. 2023

ACS Appl. Mater. Interfaces

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Combination of chemotherapeutics with polypeptide/protein drugs has been demonstrated to be an effective approach for treatment against cancer multidrug resistance. However, due to the low biostability and weak cell penetrating ability of biomacromolecules, intracellular delivery and release of biomacromolecules in a spatiotemporally controllable manner in target sites in vivo face great challenges, and synergistic effects will not be achieved as expected just by simple drug combination. Here, we conceived an inspired strategy to combat the drug-resistant tumors by fabricating multiarm PEG-gated large pore-sized mesoporous silica nanoparticles for the Bcl-2-functional-converting peptide (denoted as N9@M-CA∼8P) payload and controlled release and realizing synergistic effects with celastrol integration at a low dosage as a curative sensitizer. Our results demonstrated that the N9 peptide could be pH-responsively released from the macropores of the M-CA∼8P nanosystem both in simulated physiological environments and in cancer cells and at tumor sites. Biosafe and enhanced therapeutic outcomes (90% tumor inhibition) were obtained by combination of the N9@M-CA∼8P nanosystem with celastrol coordinatively inducing mitochondrion-mediated cell apoptosis in resistant cancer cell lines and in the corresponding xenografted mice models. Overall, this study provides convincing evidence for effective and safe resistant cancer treatment through a stimulus-responsive biomacromolecule nanosystem combined with a low dosage of a natural compound.

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Section: Methodssupporting

confidence: 56%

Section: In Vitromentioning

confidence: 99%

Section: Cytotoxicity Assaymentioning

confidence: 99%

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Acid-Responsive Macroporous Silica Nanoparticles for Bcl-2-Functional-Converting Peptide Release and Synergism with Celastrol for Enhanced Therapy against Resistant Cancer

Zhou

Lin

et al. 2023

ACS Appl. Mater. Interfaces

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“…Behind the benefits of small-sized particles, the biosafety must be considered when the particle sizes become smaller than 20 nm. 23 As MCPs with higher dispersion stability can be reversibly assembled by applying a direct current magnetic field (DCMF), the assembly state of MCPs can be controlled by the DCMF, even in an intracellular environment. Furthermore, as a weak magnetic field is harmless, an external magnetic field can be utilized as a non-invasive stimulus for the active control of particle localization in cells.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Conventional studies tend to focus on a single particle design, especially small-sized particles due to their high specific surface area, efficient cellular uptake, and mobility in cells. Behind the benefits of small-sized particles, the biosafety must be considered when the particle sizes become smaller than 20 nm …”

Section: Introductionmentioning

confidence: 99%

Intracellular Magnetic Control over the Reversible Assembly of Silica-Based Magnetic Composite Particles for Photothermal Therapy

Kameda

Suzuki

Kato

et al. 2023

ACS Appl. Nano Mater.

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Many photothermal materials have been developed in recent years to achieve efficient photothermal therapy. Although larger-sized materials are preferred to ensure a better photothermal performance, upsizing hampers the cellular uptake of these materials. To overcome this dilemma, we proposed an active control system to manipulate the assembly of magnetic composite particles (MCPs) at the cellular level. Herein, MCPs of different sizes (small, medium, and large) were synthesized, and their surfaces were modified with a polymer. These MCPs were then cultured with HeLa cells, and their uptake and mobility were investigated. Small-sized MCPs (d v = 206 nm) and medium-sized MCPs (d v = 312 nm) could be introduced into HeLa cells. However, a smaller amount of the large-sized MCPs was introduced as compared to the other MCPs. Using a direct current magnetic field [DCMF (B = 150 mT)], medium-sized MCPs were observed to quickly assemble in cells; however, redispersal did not occur after the DCMF was turned off. To improve the particle dispersibility, polyethylene glycol and polyethyleneimine (PEI) were used to modify the medium-sized MCPs. Coating the MCPs with PEI (a polymer molecular weight of 25,000 or 270,000) improved their dispersibility in phosphate-buffered saline and cellular uptake. In addition, the redispersal of assembled PEI-coated MCPs was observed after the DCMF was turned off. The photothermal conversion efficiency of the MCPs was also improved using the DCMF. Consequently, the PEI-coated MCPs were first introduced into cells in a dispersed state, and their assembly was induced at the subcellular scale by applying a DCMF, and the assembly was spontaneously redispersed by switching off the DCMF. Such a remote control could improve the cytotoxicity of MCPs under near-infrared laser irradiation.

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Coordination polysaccharide nanomissiles with size/charge dual-transformability and immunomicroenvironment-reeducating activity potentiate PD-1 blockade in hepatocellular carcinoma

Wang

Qian

et al. 2023

Chemical Engineering Journal

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Impact of macroporous silica nanoparticles at sub-50nm on bio-behaviors and biosafety in drug-resistant cancer models

Cited by 5 publications

References 39 publications

Acid-Responsive Macroporous Silica Nanoparticles for Bcl-2-Functional-Converting Peptide Release and Synergism with Celastrol for Enhanced Therapy against Resistant Cancer

Acid-Responsive Macroporous Silica Nanoparticles for Bcl-2-Functional-Converting Peptide Release and Synergism with Celastrol for Enhanced Therapy against Resistant Cancer

Intracellular Magnetic Control over the Reversible Assembly of Silica-Based Magnetic Composite Particles for Photothermal Therapy

Coordination polysaccharide nanomissiles with size/charge dual-transformability and immunomicroenvironment-reeducating activity potentiate PD-1 blockade in hepatocellular carcinoma

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