Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease

Abstract

Purpose: Although there is still no consensus on the best animal model for dry eye disease research, a model based on lacrimal gland extraction (LGE) model is widely used. In this study, we aimed to investigate the histopathological changes taking place on the contralateral eye after unilateral LGE to determine whether it is useful as a control.Methods: Seven-week-old male C57BL/6 mice were divided into naive control, environmental chamber model, and LGE groups. Corneal fluorescein staining was scored to quantify the severity of damage. Morphological changes in the cornea, conjunctiva, and lacrimal gland (LG) were determined by hematoxylin and eosin staining and compared to those on naive control animals.Results: Compared to naive subjects, the unilateral LGE model showed enhanced corneal erosion scores and loss of conjunctival goblet cells, not only on the ipsilateral but also on the contralateral side. These changes in the ocular surface became more pronounced in a time-dependent manner. Furthermore, loss of LG acinar cells and leukocyte infiltration were detected in the contralateral LGs of the LGE model.Conclusions: Considering the changes observed in the ocular surface and LGs, the contralateral side of the LGE model may not offer proper control conditions for the experimental comparison of the effects of dry eye disease in vivo. There may be regulatory feedback or crosstalk system between both eyes activated in response to LGE.

Exorbital Lacrimal Gland Ablation and Regrafting Induce Local, Corneal and Trigeminal Ganglion Inflammation but Not Regeneration or Dry Eye

“…They are in accordance with previous descriptions of regional and systemic repercussions of LG losses in mice. A recent study observed the response to LG complete ablation in mice and reported changes on the contralateral side, including inflammation of the LG, keratitis, and reduction in conjunctival goblet cells [35]. Another study with LG complete ablation in mice induced an increase of IL-1β, TNF-α, and MMP-9 mRNA in the ipsilateral cornea [28].…”