Impact of Irradiation on laryngeal hydration and lubrication in rat larynx

Lee, Song Yi; Choi, Jeong Seok; Kim, Hun Jung; Kim, Young Mo; Lim, Jae Yol

doi:10.1002/lary.25298

Cited by 7 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Direct laryngeal irradiation has shown to cause pathologic findings of increased collagen content, fibrosis, mucositis or dry mucosa, and edema of the vocal folds, as well as muscle atrophy, endothelial damage of vessels, ultimately impairing function in irradiated vocal folds 25–27 . Animal studies have also characterized the molecular changes in irradiated vocal folds 28,29 . The severity of patient reported symptoms of dysphagia and aspiration due to these radiation changes has been correlated with radiation doses to the larynx and subglottic region 30–32 .…”

Section: Discussionmentioning

confidence: 99%

“… 25 , 26 , 27 Animal studies have also characterized the molecular changes in irradiated vocal folds. 28 , 29 The severity of patient reported symptoms of dysphagia and aspiration due to these radiation changes has been correlated with radiation doses to the larynx and subglottic region. 30 , 31 , 32 Our study highlights that laryngeal dysfunction occurs even in patients who did not receive primary laryngeal irradiation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Late laryngeal dysfunction in head and neck cancer survivors

Almas,

Jeffery

2023

Laryngoscope Investig Oto

View full text Add to dashboard Cite

ObjectivesHead and neck cancer (HNC) survivorship issues are areas of increasing research interest. Laryngeal dysfunction in HNC patients is particularly important given the importance of the larynx in voice, swallowing, and airway protection. The objective of our study is to characterize late laryngeal dysfunction in a cohort of long‐term HNC survivors.MethodsHNC survivors who were at least 2 years post‐treatment were recruited prospectively for standard collection of videolaryngoscopy findings, videofluoroscopic swallowing studies, and assessment of clinical outcomes. Descriptive statistics were performed and clinical presentation and outcomes were compared between survivors >10 years and <10 years post‐treatment. Additional factor analysis to correlate clinical outcomes with clinical variables was performed.ResultsThirty participants were analyzed with a mean age of 66 years. The majority were male (80%) patients treated for oropharyngeal squamous cell carcinoma (67%). Within the cohort, 43% underwent primary chemoradiation therapy and had 13% radiation alone. Common presenting symptoms included swallowing dysfunction (83%), voice change (67%), and chronic cough (17%). Laryngeal findings on video laryngoscopy include vocal fold motion abnormalities (VFMA) in over half of participants (61%) and mucosal changes in 20%. A weak correlation was found between time since treatment and laryngeal dysfunction (r = .182, p = .34), and no correlation was found between age, sex, time since treatment, or primary site and the presence or absence of VFMA, G‐tube status, or tracheostomy‐tube status.ConclusionLate laryngeal dysfunction in HNC survivors contributes to significant morbidity, is difficult to treat, and remains static decades after treatment for their original cancer.Lay SummaryThe voice‐box, or the larynx, plays an important role in voice, swallowing and airway protection. It is particularly vulnerable to radiation‐related damage and changes. This study demonstrates the sequelae of long‐term damage of the larynx in head and cancer survivors.Level of EvidenceIV.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Late laryngeal dysfunction in head and neck cancer survivors

Almas,

Jeffery

2023

Laryngoscope Investig Oto

View full text Add to dashboard Cite

show abstract

“…A complication connected to radiation therapy in head and neck cancer is that salivary tissue is destroyed in the process and respective patients suffer severely from dry a mouth leading to ulcers, inflammation, infections, tooth decay, and swallowing difficulties (Baum et al, 2012;Lee et al, 2015). Since the atrophy of laryngeal cells is not treatable in a classical way, gene technological attempts have been made in rat irradiation models to bring AQP1 to expression and restore saliva production (Baum et al, 2012).…”

Section: Aqp Gene Replacement Therapymentioning

confidence: 98%

Challenges and achievements in the therapeutic modulation of aquaporin functionality

Beitz

Golldack

Rothert

et al. 2015

Pharmacology & Therapeutics

View full text Add to dashboard Cite

“…We also tried to further demonstrate that these specifically isolated single clonal cells actually have an effect on repairing and/or regenerating damaged laryngeal tissue, by using an irradiation-induced fibrosis animal model. Avoiding radiation exposure to the larynx is a significant clinical problem for patients with head and neck cancer, since irradiation-induced laryngeal fibrosis, edema, and desiccated mucosa result in intractable dysphonia [ 11 – 13 ]. The use of MSCs as a treatment to prevent or ameliorate damage associated with radiation may be a useful approach.…”

Section: Introductionmentioning

confidence: 99%

“…The use of MSCs as a treatment to prevent or ameliorate damage associated with radiation may be a useful approach. For these reasons, we employed our previously established irradiation-induced laryngeal dysfunction animal model to investigate regenerative potential following radiation injury [ 13 ]. Results presented herein will advance the understanding of laryngeal tissue stem/progenitor cells, and serve as a prerequisite step for development of clinical VF regenerative therapies.…”

Section: Introductionmentioning

confidence: 99%

Comparative characteristics of laryngeal-resident mesenchymal stromal cell populations isolated from distinct sites in the rat larynx

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundAlthough tissue-resident mesenchymal stromal cells (MSCs) in the larynx have been described, their distinct characteristics and roles have not been thoroughly explored. Therefore, we investigated stem cell characteristics and regenerative potentials of single clonal populations isolated from rat epiglottic mucosa (EM), lamina propria (LP), and macula flava (MF) to determine whether they comprised laryngeal tissue-resident stem cells.MethodsSingle clonal laryngeal cells were isolated following microdissection of the EM, LP, and MF from the rat larynx. Several clonal populations from the three laryngeal subsites were selected and expanded in vitro. We compared the stem cell characteristics of self-renewal and differentiation potential, as well as the cell surface phenotypes and gene expression profiles, of laryngeal MSC-like cells to that of bone marrow MSCs (BM-MSCs). We also investigated the regenerative potential of the laryngeal cells in a radiation-induced laryngeal injury animal model.ResultsSelf-renewing, clonal cell populations were obtained from rat EM, LP, and MF. EM-derived and LP-derived clonal cells had fibroblast-like features, while MF-resident clonal cells had stellate cell morphology and lipid droplets containing vitamin A. All laryngeal clonal cell populations had MSC-like cell surface marker expression (CD29, CD44, CD73, and CD90) and the potential to differentiate into bone and cartilage cell lineages; EM-derived and MF-derived cells, but not LP-derived cells, were also able to differentiate into adipocytes. Clonal cells isolated from the laryngeal subsites exhibited differential extracellular matrix-related gene expression. We found that the mesenchymal and stellate cell-related genes desmin and nestin were enriched in laryngeal MSC-like cells relative to BM-MSCs (P < 0.001). Growth differentiation factor 3 (GDF3) and glial fibrillary acidic protein (GFAP) transcript and protein levels were higher in MF-derived cells than in other laryngeal populations (P < 0.001). At 4 weeks after transplantation, laryngeal MF-derived and EM-derived cells contributed to laryngeal epithelial and/or glandular regeneration in response to radiation injury.ConclusionsThese results suggest that cell populations with MSC characteristics reside in the EM, LP, and MF of the larynx. Laryngeal MSC-like cells contribute to regeneration of the larynx following injury; further investigation is needed to clarify the differential roles of the populations in laryngeal tissue regeneration, as well as the clinical implications for the treatment of laryngeal disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0650-y) contains supplementary material, which is available to authorized users.

show abstract

Impact of Irradiation on laryngeal hydration and lubrication in rat larynx

Abstract: N/A.

Cited by 7 publications

References 22 publications

Late laryngeal dysfunction in head and neck cancer survivors

Late laryngeal dysfunction in head and neck cancer survivors

Challenges and achievements in the therapeutic modulation of aquaporin functionality

Comparative characteristics of laryngeal-resident mesenchymal stromal cell populations isolated from distinct sites in the rat larynx

Contact Info

Product

Resources

About