Impact of Iron Supply on the Kinetics of Recovery of Photosynthesis in Cd-stressed Poplar (Populus glauca)

Solti, Ádám; Gáspár, László; Mészáros, Ilona; Szigeti, Zoltán; Lévai, László; Sárvári, Éva

doi:10.1093/aob/mcn160

Cited by 58 publications

(39 citation statements)

References 55 publications

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“…The finding of decreases in calcium, iron, manganese, and zinc in cadmium-treated P. 3 canescens is consistent with previous studies Metwally et al, 2005;Solti et al, 2008;Rodríguez-Serrano et al, 2009) and probably due to competition with cadmium ions for transporters. Reduction in CO 2 assimilation is probably due to defects in the photosynthetic apparatus (McCarthy et al, 2001;Solti et al, 2008;Ivanova et al, 2011), inactivation of photosynthesis-related enzymes (Leon et al, 2002;He et al, 2011), decreases in photosynthetic pigments or iron (Solti et al, 2008;Besson-Bard et al, 2009;Wu et al, 2012), and differential expression of photosynthesis-related genes and proteins (Kieffer et al, 2009a(Kieffer et al, , 2009bDurand et al, 2010). Reduced CO 2 assimilation often leads to changes in the concentrations of sugars and sugar alcohols in cadmium-treated plants (Devi et al, 2007;Kieffer et al, 2009a;He et al, 2011).…”

Section: Changed Nutrient and Carbohydrate Concentrations And Shiftedsupporting

confidence: 92%

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

et al. 2013

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Bark tissue of Populus 3 canescens can hyperaccumulate cadmium, but microstructural, transcriptomic, and physiological response mechanisms are poorly understood. Histochemical assays, transmission electron microscopic observations, energydispersive x-ray microanalysis, and transcriptomic and physiological analyses have been performed to enhance our understanding of cadmium accumulation and detoxification in P. 3 canescens. Cadmium was allocated to the phloem of the bark, and subcellular cadmium compartmentalization occurred mainly in vacuoles of phloem cells. Transcripts involved in microstructural alteration, changes in nutrition and primary metabolism, and stimulation of stress responses showed significantly differential expression in the bark of P. 3 canescens exposed to cadmium. About 48% of the differentially regulated transcripts formed a coregulation network in which 43 hub genes played a central role both in cross talk among distinct biological processes and in coordinating the transcriptomic regulation in the bark of P. 3 canescens in response to cadmium. The cadmium transcriptome in the bark of P. 3 canescens was mirrored by physiological readouts. Cadmium accumulation led to decreased total nitrogen, phosphorus, and calcium and increased sulfur in the bark. Cadmium inhibited photosynthesis, resulting in decreased carbohydrate levels. Cadmium induced oxidative stress and antioxidants, including free proline, soluble phenolics, ascorbate, and thiol compounds. These results suggest that orchestrated microstructural, transcriptomic, and physiological regulation may sustain cadmium hyperaccumulation in P. 3 canescens bark and provide new insights into engineering woody plants for phytoremediation.

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Section: Changed Nutrient and Carbohydrate Concentrations And Shiftedsupporting

confidence: 92%

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

et al. 2013

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“…Whereas the concentration of Fe did not changed significantly in the leaves, the chloroplast-directed re-distribution of Fe may have been of special importance in the hardening process. Any increase in the chloroplast Fe content results in an increase of the strongly Fe-dependent processes such as the biosynthesis of Chls and the formation of Fe-containing PSI complexes [26]. In addition to the increase in Φ PSII , the observed increase in the β-carotene/ Chl and Chl a/b ratios indicates a de novo formation of reaction centres.…”

Section: Changes In Excitation Energy Allocation During Stress Hardeningmentioning

confidence: 98%

“…Carotenoid components were separated by HPLC method and quantified using zeaxanthin as standard [25,26]. The de-epoxidation state of xanthophyll cycle pigments (DEEPS) was calculated as (DEEPs = Z + 0.5A)/(V + A + Z) and diurnal change as ΔDEEPS = DEEPS light -DEEPS dark .…”

Section: Pigment Contentsmentioning

confidence: 99%

Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus

Solti

Sárvári

Szöllősi

et al. 2016

Zeitschrift Für Naturforschung C

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Cadmium (Cd), a highly toxic heavy metal affects growth and metabolic pathways in plants, including photosynthesis. Though Cd is a transition metal with no redox capacity, it generates reactive oxygen species (ROS) indirectly and causes oxidative stress. Nevertheless, the mechanisms involved in long-term Cd tolerance of poplar, candidate for Cd phytoremediation, are not well known. Hydroponically cultured poplar (Populus jacquemontiana var. glauca cv. 'Kopeczkii') plants were treated with 10 μM Cd for 4 weeks. Following a period of functional decline, the plants performed acclimation to the Cd induced oxidative stress as indicated by the decreased leaf malondialdehyde (MDA) content and the recovery of most photosynthetic parameters. The increased activity of peroxidases (PODs) could have a great impact on the elimination of hydrogen peroxide, and thus the recovery of photosynthesis, while the function of superoxide dismutase (SOD) isoforms seemed to be less important. Re-distribution of the iron content of leaf mesophyll cells into the chloroplasts contributed to the biosynthesis of the photosynthetic apparatus and some antioxidative enzymes. The delayed increase in photosynthetic activity in relation to the decline in the level of lipid peroxidation indicates that elimination of oxidative stress damage by acclimation mechanisms is required for the restoration of the photosynthetic apparatus during long-term Cd treatment.

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“…Fluorescence induction measurements of leaf samples were performed using a PAM 101-102-103 Chlorophyll Fluorometer (Walz, Effeltrich, Germany) according to Solti et al (2008). Maximal quantum efficiency of the photosystem II (PSII) F v /F m = (F m -F 0 )/F m was used to monitor the physiological status of the leaves.…”

Section: Leaf Fluorescence Measurementsmentioning

confidence: 99%

“…Fluorescence imaging was performed by a compact flashlamp imaging system (Szigeti, 2008) according to Solti et al (2008). Shortly after, excitation of the adaxial side of the lightadapted leaves was carried out by xenon-lamp flashes (k exc = 360-370 nm) at room temperature and the detection of fluorescence was performed by a CCD video camera at different wavelengths using appropriate interference filters.…”

Section: Leaf Fluorescence Measurementsmentioning

confidence: 99%

Cd, Fe, and Light Sensitivity: Interrelationships in Cd-Treated Populus

Solti

Gáspár

Vági

et al. 2011

OMICS: A Journal of Integrative Biology

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Cadmium is a toxic heavy metal causing iron deficiency in the shoot and light sensitivity of photosynthetic tissues that leads to decreased photosynthetic performance and biomass production. Light intensity had strong impact on both photosynthetic activity and metal accumulation of cadmium-treated plants. At elevated irradiation, cadmium accumulation increased due to the higher dry mass of plants, but its allocation hardly changed. A considerable amount of iron accumulated in the roots, and iron concentration was higher in leaves developed at moderate rather than low irradiation. At the same time, the higher the irradiation the lower the maximal photochemical quantum efficiency. The decreased photochemical efficiency, however, started to recover after a week of Cd treatment at moderate light without substantial change in metal concentrations but following the accumulation of green fluorescent compounds. Both cadmium treatment and higher light caused the accumulation of flavonoids in leaf mesophyll vacuoles/chloroplasts, but accumulation of flavonols, fluorescing at 510 nm, was characteristic to cadmium stress. Therefore, flavonoids, which may act by scavenging reactive radicals, chelating Cd, and shielding against excess irradiation, play an important part in Cd stress tolerance of Populus, and may have special impact on its phytoremediation capacity.

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Impact of Iron Supply on the Kinetics of Recovery of Photosynthesis in Cd-stressed Poplar (Populus glauca)

Abstract: Iron deficiency is a key factor in Cd-induced inhibition of photosynthesis.

Cited by 58 publications

References 55 publications

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus

Cd, Fe, and Light Sensitivity: Interrelationships in Cd-Treated Populus

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