Impact of iron dextran on polymorphonuclear cell function among hemodialysis patients

Guo, Daoxia; Jaber, Bertrand L.; Lee, S; Perianayagam, Mary C.; King, AJ; Pereira, Brian J.G.; Balakrishnan, Vaidyanathapuram S.

doi:10.5414/cnp58134

Cited by 30 publications

(25 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study showed that PMN from hemodialysis patients who were treated with low-dose iron sucrose (10 mg three times weekly after hemodialysis) and a ferritin level Ͼ650 g/L presented with impaired intracellular killing as well as phagocytosis, as compared with PMN harvested from healthy subjects. Furthermore, it was demonstrated that iron dextran attenuates PMN function in hemodialysis patients even without signs of iron overload and with normal iron indices at clinically relevant concentrations (40). No such studies are currently available for iron gluconate.…”

Section: Discussionmentioning

confidence: 99%

Impairment of Transendothelial Leukocyte Migration by Iron Complexes

Sengoelge

Kletzmayr

Ferrara

et al. 2003

Journal of the American Society of Nephrology

View full text Add to dashboard Cite

Abstract. Although iron sucrose and iron gluconate are generally well tolerated in patients who are treated for renal anemia, recent clinical studies and cell culture experiments suggested significant toxicity and long-term side effects arising from the use of these iron complexes. Because of the possible role of iron in infection or cardiovascular disease, it was theorized that parenteral iron compounds influence endothelial and PMN interaction in vitro. A well-established double-chamber method was used to assess the effect of different concentrations of iron sucrose and iron gluconate (1, 25, 50, and 100 g/ml) on the transendothelial migration of PMN. Preincubation of PMN and endothelial cells as well as preincubation of PMN alone with 25, 50, or 100 g/ml iron resulted in a significant decrease in PMN migration. In contrast, after incubation of the endothelial cells alone with iron, no reduction in the transendothelial migration of PMN was observed. Preincubation of PMN and/or endothelial cells with 1 g/ml iron did not lead to any decrease in the rate of migrated PMN. The only significant change in experiments with 1 g/ml was an increase in PMN migration after preincubation of endothelial cells and PMN with iron gluconate. A four-way ANOVA showed a significant effect of the iron concentration (P Ͻ 0.000001), of type of iron complex (P Ͻ 0.005), of the preincubation of endothelial cell (P Ͻ 0.001), and of the preincubation of PMN with iron (P Ͻ 0.000001) on PMN diapedesis. It is concluded that iron sucrose and iron gluconate cause a significant inhibition of transendothelial migration of PMN.Renal anemia therapy requires an intravenous iron substitution in addition to the erythropoietin therapy in the majority of patients (1). Iron substitution not only reduces the erythropoietin dosage needed but also is necessary to maintain the target hemoglobin above 11 g/dl (2,3). There are several iron preparations for intravenous use available, all of which have potential side effects, such as allergic reactions, cell injury, or endothelial dysfunction (4 -7). Moreover, iron therapy may be associated with infectious complications and with loss of the ability of patient serum to resist the bacterial growth (8 -10). PMN play a vital role in the nonspecific immune reaction against bacterial infections executing functions such as chemotaxis, transendothelial migration, phagocytosis, and intracellular killing by proteolytic enzymes or toxic oxygen radicals. Although the effects of iron on chemotaxis of PMN, phagocytosis, and intracellular killing in PMN were studied previously, the effect of iron complexes on PMN-endothelial cell interaction is unknown. Therefore, we examined the effect of incubation of PMN and/or endothelial cells with two widely used iron complexes, iron(III)-hydroxide-sucrose complex (iron sucrose) and iron(III)-sodium-gluconate in sucrose (iron gluconate), on the PMN migration through the endothelium in an in vitro setting.

show abstract

Section: Discussionmentioning

confidence: 99%

Impairment of Transendothelial Leukocyte Migration by Iron Complexes

Sengoelge

Kletzmayr

Ferrara

et al. 2003

Journal of the American Society of Nephrology

View full text Add to dashboard Cite

show abstract

“…Finally, elevated free iron is known to have a profound and detrimental effect on immune cells necessary for combating mucormycosis. Specifically, elevated concentrations of iron were found to be toxic to phagocytes (19)(20)(21), and spleen cells from mice fed excess levels of iron secrete less IFN-γ (22), a cytokine that upregulates killing of several Mucorales family members (including R. oryzae) by human neutrophils (23). Additionally, BHB, which has been reported to abrogate the formation of bovine neutrophil extracellular traps and their bactericidal activity against pathogen E. coli (9), attenuated the ability of human neutrophils to kill R. oryzae ex vivo by reducing ROS production.…”

Section: Discussionmentioning

confidence: 99%

Bicarbonate correction of ketoacidosis alters host-pathogen interactions and alleviates mucormycosis

Gebremariam¹,

Lin²,

Liu³

et al. 2016

Journal of Clinical Investigation

127

View full text Add to dashboard Cite

“…Inhibition of bactericidal activity of PMN in the presence of FeSO 4 has also been demonstrated (Gladstone and Walton 1971). Guo et al (2002) have reported significant impairment of phagocyte oxidative metabolism of PMN from patients on haemodialysis.…”

Section: Iron Dextran Reactive Oxygen Species Antioxidantmentioning

confidence: 99%

“…However, studies evaluating oxidative stress induced by iron dextran in human patients are based mainly on in vitro studies (Guo et al 2002). Therefore experiments conducted on a swine model may provide valuable information.…”

Section: Iron Dextran Reactive Oxygen Species Antioxidantmentioning

confidence: 99%

See 1 more Smart Citation

Effect of an Acute Iron Overdose on PMN Cells Chemiluminescence and Indices of Inner Environment in a Swine Model

et al. 2006

View full text Add to dashboard Cite

The objective of the study was to evaluate the effect of an acute iron dextran administration on polymorphonuclear cells chemiluminescence and indices of inner environment in piglets. Piglets in the experimental group (n = 16) were given 2000 mg (20 ml) Fe 3+ in the form of iron dextran i.m. Piglets in the control group (n = 13) were given the same amount of physiological saline. Blood was taken before administration, and 1, 4 and 24 hours after. Iron application resulted in a ten times higher concentration of iron in blood plasma in the experimental group. No differences in spontaneous, PMA-and OZP-stimulated chemiluminescence were recorded between the two groups. The increased activities of CK, AST and LDH are indicative of a muscle injury at the injection site. No changes indicating liver or kidney injury were reported. Our study provides evidence of a good safety of iron dextran after parenteral administration. Iron dextran, reactive oxygen species, antioxidant

show abstract

Impact of iron dextran on polymorphonuclear cell function among hemodialysis patients

Cited by 30 publications

References 0 publications

Impairment of Transendothelial Leukocyte Migration by Iron Complexes

Impairment of Transendothelial Leukocyte Migration by Iron Complexes

Bicarbonate correction of ketoacidosis alters host-pathogen interactions and alleviates mucormycosis

Effect of an Acute Iron Overdose on PMN Cells Chemiluminescence and Indices of Inner Environment in a Swine Model

Contact Info

Product

Resources

About