Impact of Inorganic Xenograft on Bone Healing and Osseointegration

Munhoz, Etiene Andrade; Bodanezi, Augusto; Cestari, Biol Tania Mary; Graeff, Márcia Sirlene Zardin; Ferreira, Olga; Carvalho, P.S.P. de; Taga, Rumio

doi:10.1097/id.0000000000000694

Cited by 2 publications

(4 citation statements)

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“…The surgical site was easily accessible through the conventional intraoral approach. There was minimal blood loss and low morbidity compared to other dental implant models as no extraction of primary teeth, lateralization of the inferior alveolar nerves or excessive bone removal were required [ 16 , 35 – 42 ]. The presented model was designed considering the three fundamental principles of animal research; replacement, reduction and refinement.…”

Section: Discussionmentioning

confidence: 99%

“…However, in many of these models the implants were placed in extraoral locations. Dental implants have been placed in the extraction sockets of the rabbit mandibular teeth by a handful of authors [ 16 , 37 , 39 – 42 ]. Munhoz et al in 2017 evaluated the impact of xenograft (Gen-Ox) on the osseointegration of dental implants after the extraction of the lower incisors bilaterally [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Dental implants have been placed in the extraction sockets of the rabbit mandibular teeth by a handful of authors [ 16 , 37 , 39 – 42 ]. Munhoz et al in 2017 evaluated the impact of xenograft (Gen-Ox) on the osseointegration of dental implants after the extraction of the lower incisors bilaterally [ 16 ]. The removal of the lower incisors may, however, have compromised the animal welfare, the rabbits are lagomorph animals which are entirely herbivorous, and the lower incisors are crucial for chewing and biting of food [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Another added advantage is the fact that the rabbit is classified as a small animal and so can be housed in a small animal facility, but it is large enough to allow the testing of the osseointegration of dental implants. Rabbits are considered a reliable model for studies related to bone regeneration, periodontal wound healing and the integration of dental implants [ 10 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

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A novel surgical model for the preclinical assessment of the osseointegration of dental implants: a surgical protocol and pilot study results

et al. 2021

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Background Dental implants are considered the gold standard replacement for missing natural teeth. The successful clinical performance of dental implants is due to their ability to osseointegrate with the surrounding bone. Most dental implants are manufactured from Titanium and it alloys. Titanium does however have some shortcomings so alternative materials are frequently being investigated. Effective preclinical studies are essential to transfer the innovations from the benchtop to the patients. Many preclinical studies are carried out in the extra-oral bones of small animal models to assess the osseointegration of the newly developed materials. This does not simulate the oral environment where the dental implants are subjected to several factors that influence osseointegration; therefore, they can have limited clinical value. Aim This study aimed to develop an appropriate in-vivo model for dental implant research that mimic the clinical setting. The study evaluated the applicability of the new model and investigated the impact of the surgical procedure on animal welfare. Materials and methods The model was developed in male New Zealand white rabbits. The implants were inserted in the extraction sockets of the secondary incisors in the maxilla. The model allows a split-mouth comparative analysis. The implants’ osseointegration was assessed clinically, radiographically using micro-computed tomography (µ-CT), and histologically. A randomised, controlled split-mouth design was conducted in 6 rabbits. A total of twelve implants were inserted. In each rabbit, two implants; one experimental implant on one side, and one control implant on the other side were applied. Screw-shaped implants were used with a length of 8 mm and a diameter of 2 mm. Results All the rabbits tolerated the surgical procedure well. The osseointegration was confirmed clinically, histologically and radiographically. Quantitative assessment of bone volume and mineral density was measured in the peri-implant bone tissues. The findings suggest that the new preclinical model is excellent, facilitating a comprehensive evaluation of osseointegration of dental implants in translational research pertaining to the human application. Conclusion The presented model proved to be safe, reproducible and required basic surgical skills to perform.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel surgical model for the preclinical assessment of the osseointegration of dental implants: a surgical protocol and pilot study results

et al. 2021

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Comparison between fractal analysis and radiopacity evaluation as a tool for studying repair of an osseous defect in an animal model using biomaterials

Gomes

Albergaria

Henriques

et al. 2019

Dentomaxillofacial Radiology

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Objectives: To evaluate bone repair of an osseous defect in a rat animal model through fractal analysis and radiopacity analysis in radiographic images. Methods: 120 rats were subjected to extraction of their first molar and divided into four groups (n = 6/group) according to the material used for bone grafting: mineralized bovine bone, demineralized bovine bone (DBB), blood clot (BC - control) or Bio-Oss® (BO). The animals were sacrificed after 1, 7, 14, 21 and 49 days and subjected to radiographic evaluation. For fractal analysis (FA), a square regionof interest of 30 × 30 pixels was used, and radiopacity was measured as the mean gray scale (MGS) value for three points of 5 × 5 pixels in the apical, medial and coronal regions of the defect. Histomorphometric evaluation was realized as the gold standard for bone neo-formation and maturation of the new osseous matrix. Results: Histomorphometric evaluation suggested that DBB showed faster mineralized deposition and resulted in more mature bone at the final time point of evaluation. Mineralized bovine bone and Bio-Oss presented similar results. The mineralized groups did not show significant differences in bone maturation. The radiopacity analysis revealed a significant difference (p < 0.05) between the DBB and blood clot groups at the final time point. FA did not show any significant differences at the final time point. Conclusions: Mean gray scale seemed to be more effective for the quantification of bone repair than FA in the demineralized group in this animal model. Results for the mineralized groups did not reveal a significant difference, leading to the conclusion that both methods are effective.

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Impact of Inorganic Xenograft on Bone Healing and Osseointegration

Abstract: The bone healing or the course of osseointegration was not impaired by the use of an inorganic xenogenic graft before insertion of a titanium implant.

Cited by 2 publications

References 28 publications

A novel surgical model for the preclinical assessment of the osseointegration of dental implants: a surgical protocol and pilot study results

A novel surgical model for the preclinical assessment of the osseointegration of dental implants: a surgical protocol and pilot study results

Comparison between fractal analysis and radiopacity evaluation as a tool for studying repair of an osseous defect in an animal model using biomaterials

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