Impact of innervation and exercise on muscle regeneration in neovascularized muscle grafts in rats

Noah, Ernst Magnus; Winkel, R.; Schramm, Uda; Kühnel, Wolfgang

doi:10.1016/s0940-9602(02)80018-8

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…There is evidence that aggressive exercise immediately after surgical implantation of ECM scaffolds to VML injuries has significant benefit on matrix remodelling1454. Moreover, positive effects of exercise have been observed within 30 days following muscle grafting in rats, resulting in improved re-innervation535455. In agreement with these reports, when we followed treatment of VML injuries with bioconstructs and exercise, we found enhanced maturation of innervation and enhanced force production in vivo at 30 days following treatment.…”

Section: Discussionsupporting

confidence: 85%

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss

et al. 2017

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Volumetric muscle loss (VML) is associated with loss of skeletal muscle function, and current treatments show limited efficacy. Here we show that bioconstructs suffused with genetically-labelled muscle stem cells (MuSCs) and other muscle resident cells (MRCs) are effective to treat VML injuries in mice. Imaging of bioconstructs implanted in damaged muscles indicates MuSCs survival and growth, and ex vivo analyses show force restoration of treated muscles. Histological analysis highlights myofibre formation, neovascularisation, but insufficient innervation. Both innervation and in vivo force production are enhanced when implantation of bioconstructs is followed by an exercise regimen. Significant improvements are also observed when bioconstructs are used to treat chronic VML injury models. Finally, we demonstrate that bioconstructs made with human MuSCs and MRCs can generate functional muscle tissue in our VML model. These data suggest that stem cell-based therapies aimed to engineer tissue in vivo may be effective to treat acute and chronic VML.

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Section: Discussionsupporting

confidence: 85%

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss

et al. 2017

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“…Decherchi and Dousset32 showed that central neuron activity adaptation to exercise is activated by exercise‐induced changes in muscle metabolism. In another study, alterations in denervated muscle, such as in histochemically stained muscle fiber, and enzyme activities after treadmill training were observed 33. When lower motor neuron weakness occurred, the CMAP amplitude was determined either by the nerve, the integrity of the neuromuscular junction, or the quality of the muscle innervated by the nerve.…”

Section: Discussionmentioning

confidence: 98%

Effects of treadmill running on rat gastrocnemius function following botulinum toxin A injection

Tsai

Chen

et al. 2011

Journal Orthopaedic Research

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Exercise can improve and maintain neural or muscular function, but the effects of exercise in physiological adaptation to paralysis caused by botulinum toxin A has not been well studied. Twenty-four rats were randomly assigned into control and treadmill groups. The rats assigned to the treadmill group were trained on a treadmill three times per week with the running speed set at 15 m/min. The duration of training was 20 min/session. Muscle strength, nerve conduction study and sciatic functional index (SFI) were used for functional analysis. Treadmill training improved the SFI at 2, 3, and 4 weeks (p = 0.01, 0.004, and 0.01, respectively). The maximal contraction force of the gastrocnemius muscle in the treadmill group was greater than in the control group (p < 0.05). The percentage of activated fibers was higher in the treadmill botox group than the percentage for the control botox group, which was demonstrated by differences in amplitude and area of compound muscle action potential (CMAP) under the curve between the groups (p < 0.05). After BoNT-A injection, treadmill improved the physiological properties of muscle contraction strength, CMAP amplitude, and the recovery of SFI.

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“…Since blood circulation is thought to play an important role in muscle repair [9][10][11][12][13] (see introduction), we therefore hypothesized that peripheral arterial insufficiency would markedly reduce muscle recovery after myotoxic injury. The present study demonstrates for the first time that artery ligation has a strong detrimental effect on muscle recovery after myotoxic injury in mice.…”

Section: Effect Of Hindlimb Ischemia On Regenerating Musclesmentioning

confidence: 99%

“…In favor of such a role is the impairment of muscle regeneration by the administration of a hemorrhagic agent [12]. Revascularization, i.e., muscle blood supply, might also be considered critical, since the centripetal gradient of muscle regeneration stages (from the periphery toward the center of the muscle) is observed in terminal devascularized muscle graft and is reversed when graft is wrapped around vessels [13]. To our knowledge, no study has analyzed the effect of femoral artery ligation on the functional recovery of hindlimb muscles that are treated by myotoxic agents.…”

mentioning

confidence: 99%

Sustained Peripheral Arterial Insufficiency Durably Impairs Normal and Regenerating Skeletal Muscle Function

Hourdé¹,

Vignaud

Beurdy

et al. 2006

J. Physiol. Sci

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Peripheral vascular occlusive diseases are frequently observed in humans, and studies with animal models have been largely used. However the effects of sustained lower limb ischemia on normal and regenerating hindlimb skeletal muscles are not well known in the mouse model. Therefore prolonged unilateral hindlimb ligation was generated by femoral artery ligation. Normal (myotoxic-untreated) and regenerating (myotoxicreated) ischemic muscles were studied by analyses of the in situ contractile properties and histological parameters. Concerning normal mouse muscles, we found that femoral artery ligation reduced hindlimb perfusion and altered muscle structure and function. Thus 7 days after ligation, maximal tetanic force was reduced by about 70%, (p < 0.05). By 56 days after ligation, muscle weights and cross-section areas of muscle fibers were still reduced (p < 0.05). Concerning myotoxic treated muscles, we report that ligation reduced the recovery of muscle weight and maximal tetanic force and increased fatigue resistance at 56 days (p < 0.05). In conclusion, our results demonstrate that sustained peripheral arterial insufficiency in mice induces long-term as well as acute detrimental effects in both normal and regenerating muscles.

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Impact of innervation and exercise on muscle regeneration in neovascularized muscle grafts in rats

Cited by 11 publications

References 29 publications

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss

Effects of treadmill running on rat gastrocnemius function following botulinum toxin A injection

Sustained Peripheral Arterial Insufficiency Durably Impairs Normal and Regenerating Skeletal Muscle Function

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