Impact of increased serum 8-hydroxy-2′-deoxyguanosine levels on extent of coronary artery lesions in elderly patients with type 2 diabetes

Abstract

Objective Patients with type 2 diabetes (T2DM) are prone to cardiovascular disease, and both conditions are linked to oxidative DNA damage, which produces 8-hydroxy-2′-deoxyguanosine (8-OHdG). We investigated the impact of 8-OHdG on coronary heart disease (CHD) in elderly patients with T2DM. Methods We assessed the demographic, clinical, and biochemical characteristics of 147 patients with T2DM (mean age 73.29 ± 8.19 years) with or without CHD. Serum 8-OHdG was detected by enzyme-linked immunosorbent assay. CHD was diagnosed as ≥50% stenosis in at least one main branch of the coronary arteries determined by coronarography, evaluated by Gensini score. Results Serum 8-OHdG, number of stenotic branches, and Gensini score were all significantly increased in the CHD group. After adjustment for various factors, the number of stenotic branches and Gensini score remained positively correlated with 8-OHdG levels in the CHD group. Coronary artery lesions were significantly more severe in the CHD compared with the non-CHD group when 8-OHdG levels were >0.523 ng/mL. The number of stenotic branches and Gensini score were significantly independently associated with 8-OHdG levels in patients with T2DM. Conclusions 8-OHdG is a marker of oxidative DNA damage, and is highly associated with the extent of coronary artery lesions in ageing patients with T2DM. Trial registration: Registration number: 1.0/20170720; date of registration 26/07/2016 (retrospectively registered).

DNA damage and arterial hypertension. A systematic review and meta-analysis

“…4B, Table 1, 2) (ref. [8][9][10][11][12][13][14][15][16][17][19][20]22 ). Meta-analysis showed an average mean difference between hypertensive patients and healthy controls of 7.5 (6.6; 8.6) ng/mg creatinine for 8OHdG and 14.7 (6.4; 23.0) from the Comet assay (Fig.…”

“…In contrast to comet assay, 8OHdG was also tested in a group without any specification of treatment (3 studies; N=139 pts) (ref. 14,15,22 ), and in a group with treated HT (3 studies; N=126 pts) (ref. 18,19,21 ).…”

“…The effects of different substances (especially vasodilators, vasoconstrictors, mechanical interventions) on different DNA damage types in HT animals were tested [24][25][26][27][28][29][30][31][32][33] . Regardless of antihypertensive pharmacotherapy, a majority of the selected studies demonstrated significantly higher DNA damage in HT 731 pts (349 pts; comet assay; blood) and (382 pts; 8OHdG; 122 in blood and 260 in urine) compared to controls [8][9][10][11][12][13][14][15][16][17]19,20,22 .…”

“…10,13,16,17 ), non-dippers 11 , elderly 19 , CHD (ref. 22 ) (Table 3). Unfortunately, data are based on small pts groups (10 1 -10 2 of pts), and small numbers of studies (1 0 ) usually written by the same author team.…”