Impact of Ileal Transposition Surgical Intervention on Antioxidant Status Measured in Liver Tissue of Obese Zucker Rats (Crl:ZUC‐Lepr^fa)

Abstract

Background The main factor characteristic for low-grade systemic inflammation typical for obesity is oxidative stress (OS). Reactive oxygen species (ROS) production is higher and more increased in time in the obese patients than in lean subjects. Aims To assess the effect of ileal transposition (IT) and sham types of bariatric procedures on the antioxidative systems in the liver tissue of Zucker rats (Crl:ZUC Leprfa). Method 21 animals were divided into the experimental groups: control group (n = 7), sham group (n = 7), and IT group (n = 7). Sham and IT animals underwent selected surgery. The concentration of total antioxidant capacity (TAC), total antioxidant status (TOS), and activity of glutathione reductase (glutathione-disulfide reductase, GR, GSR), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and total superoxide dismutase activity (SOD) were assessed in liver tissue 3 months after surgery. Results IT procedure significantly increased TAC when compared to sham and the control group. Animals after IT showed higher levels of TOS when compared to sham procedure. The total amount of TOS was similar in IT and control groups. GPx activity was increased in the groups submitted to the sham and IT surgery in relation to control. GR and CAT activities were significantly higher after IT in comparison to control and sham procedures. Total SOD and MnSOD were significantly higher in sham-operated animals in comparison to IT intervention and control groups. Conclusions IT procedure had a positive impact on the diminishing of oxidative stress measured by TAC and TOS markers. The dynamic, adaptive, and protective mechanisms of enzymatic antioxidant systems were observed after the IT but not sham procedure. Nevertheless, 3 months after surgery, the midterm effect of bariatric surgery was observed, which might not fully balance the antioxidative response.

General, 21-Day Postoperative Rehabilitation Program Has Beneficial Effect on Oxidative Stress Markers in Patients after Total Hip or Knee Replacement

“…This intensifies mitochondrial energy metabolism, which enhances free radicals formation [ 12 , 13 ]. The intensity of oxidative stress can be assessed using various general and specific indicators [ 14 – 16 ]. Total antioxidant capacity (TAC) shows the interaction between synergistic and antagonistic antioxidants.…”

Reverse Transposition Of The Ileum Is A New Surgical Model For The Study Of The «Hindgut" Hypothesis In Diabetes Mellitus

“…We present a novel experimental surgical procedure to investigate the role of the ileum in diabetes mellitus and the Hindgut hypothesis. As in most similar previous studies, surgical technique proposed by us is based on the movement of the ileum in the proximal direction and its location after the ligament of Treitz and duodenum, but there is not only interposition of the terminal segment of the ileum [12,13,14], but also reverse transposition -antiperistaltic location of almost the entire small intestine.…”

Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl:ZUC(ORL)-Leprfa) rats

“…Therefore, the continuous research on the metabolic shifts and their pathophysiological mechanisms after different types of bariatric surgery is still needed. Despite wide research in the animal model, the effects of ileal transposition, type of bariatric surgery, are not fully studied yet but based on the current knowledge 24 – 26 , we hypothesised that IT would have a beneficial effect on the glucose metabolism and inflammatory processes measured by selected hepatokines. We studied the effects of IT protocol on RBP4, aHSG/fetuin-A, FGF21, and CRP levels, liver histology, HOMA-IR as well as total lipid content in the obese Zucker (Crl:ZUC(ORL)-Lepr fa ) rats—an animal model of insulin resistance, glucose intolerance, metabolic syndrome, and genetic obesity 27 .…”