Sustainable agriculture aims to provide food needs while improving soil health and protecting it from degradation and contamination from excessive chemical fertilizer use. Sandy-textured soils have low fertility and water-holding capacity. This study assessed the integrated impact of super absorbent polymers (SAPs) and biofertilizer application on the soil chemical characteristics and wheat growth parameters in sandy loam soil. Two super absorbent polymers (SAPs) included Barbary plant G3 (P1) and Aqua Gool polymer (P2), and four microbial inoculations (Trichoderma harzianum (T), Actinomycetes (Streptomyces rochei and Streptomyces atrovirens) (AC1 and AC2), and Bacillus subtilis (B)) as biofertilizers were used in our pot experiment. The SAPs were applied to soil at a level of 0.2% (w/w), while biofertilizers were applied in the form of microbial cell suspensions (50 ml per pot) in addition to treating wheat seed with these suspensions during cultivation. Wheat plants were irrigated every 8 days to field capacity level. Amending soil with super absorbent polymers and microbes either individually or in combination significantly reduced pH and EC, increased organic matter level, and the availability of macro- and micronutrients in soil. Wheat growth metrics, including shoot length, tiller number, biomass accumulation, leaf area, and grain yield, exhibited considerable enhancements relative to the plants of the control treatment. The interaction between P1 polymer and Streptomyces atrovirens (AC2) showed the highest performance in improving the almost studied parameters. The application of SAPs with microbial biofertilizers offers a promising eco-friendly method for enhancing soil health and wheat yield.