Impact of Holder pasteurization on immunological properties of human breast milk over the first year of lactation

Rodríguez-Camejo, Claudio; Puyol, Arturo; Fazio, Laura; Villamil, Emilia; Arbildi, Paula; Sóñora, Cecilia; Castro, Mara; Carroscia, Lilián; Hernández, Ana

doi:10.1038/s41390-019-0500-y

Cited by 18 publications

(25 citation statements)

References 36 publications

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“…These results are in line with a higher level of most immunological factors in colostrum than in mature milk with a role to protect the newborn infant exposed to a great number of environmental factors after birth (Bardanzellu et al, 2020;Moles et al, 2015;Sahin et al, 2020). Furthermore, heat treatment of donor human milk by Holder pasteurization increased AF-compleasome after pasteurization, suggesting a sustained anti-inflammatory effect even though many bioactive components are known to be reduced or destroyed (Rodríguez-Camejo et al, 2020). The composition of human milk changes during lactation-both macronutrients and bioactive factors-with higher levels of many components in preterm milk (Moles et al, 2015;Trend et al, 2016).…”

Section: Discussionsupporting

confidence: 73%

“…The analysis of the anonymized human milk from donors demonstrated higher AF levels after Holder pasteurization compared to the analysis done before. This was an unexpected result since pasteurization is known to abolish or reduce many of the bioactive components in human milk (Rodríguez-Camejo et al, 2020). AF is activated in complex with complement factor C3, converting C3 to the inactive form C3c (Lönnroth et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…If MOM is not available, pasteurized donor human milk is often given. However, pasteurization affects many of the bioactive components in human milk and abolishes or reduces their activity (Rodríguez-Camejo et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

et al. 2021

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Background Preterm infants are more susceptible to inflammatory complications than term infants. Human milk contains numerous bioactive components protecting the newborn infant. Antisecretory factor, a protein regulating secretory and inflammatory processes by complex binding with complement factors, is present in human milk. Research Aims To describe antisecretory factor (1) in mother’s own milk in term and preterm infants; and (2) in donor milk before and after Holder pasteurization. Methods The study was prospective, longitudinal, explorative, and descriptive. Antisecretory factor-compleasome was determined using sandwich enzyme-linked immunosorbent assay in longitudinal human milk samples over 12 weeks from mothers ( N = 87) of term ( n = 41) and of preterm ( n = 46) infants and 20 anonymized donor human milk samples before and after Holder pasteurization. Results Antisecretory factor-compleasome was overall higher in colostrum versus mature milk ( p < .001) and no difference was found in term or preterm colostrum ( p = .82). In mature milk, compleasome was higher and more variable in the preterm group ( p = .01). After Holder pasteurization, compleasome levels increased ( p < .001). Conclusion Antisecretory factor followed the pattern of other immunological factors with high levels in colostrum. After preterm birth, levels of antisecretory factor were higher and more variable in mature milk. Holder pasteurization did not degrade antisecretory factor, indicating preserved anti-inflammatory properties in donor human milk.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

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Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

et al. 2021

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“…For analysis of immune components, the aqueous fraction (AF) of HM was separated for each sample as previously described. Briefly, once thawed the samples were centrifuged at 4°C in 2 sequential steps to obtain the AF: 1,000 rpm for 10 min and then 10,000 rpm for 30 min to remove the lipid phase (Rodríguez-Camejo et al, 2020). ImmunoglobulinA, IgM, and IgG were quantified in quadruplicate wells by ELISA.…”

Section: Determination Of Immunoglobulin Contentmentioning

confidence: 99%

“…The bioactive properties of milk are critical for preterm newborns (born before wk 37 of gestation), who are highly susceptible to infections and inflammatory diseases (Pineda, 2011). During hospitalization at intensive care units, preterm mothers may not be able to provide enough milk (Eidelman and Schanler, 2012), and stored HM is the best alternative, even better than bovine milk formulas (Arnold, 2006).…”

Section: Introductionmentioning

confidence: 99%

High-pressure homogenization and high hydrostatic pressure processing of human milk: Preservation of immunological components for human milk banks

Irazusta

Rodríguez-Camejo

Jorcin

et al. 2020

Journal of Dairy Science

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Human milk (HM) constitutes the first immunological barrier and the main source of nutrients and bioactive components for newborns. Immune factors comprise up to 10% of the protein content in HM, where antibodies are the major components (mainly IgA, IgG, and IgM). In addition, antibacterial enzymes such as lysozyme and immunoregulatory factors such as soluble cluster of differentiation 14 (sCD14) and transforming growth factor β2 (TGF-β2) are also present and play important roles in the protection of the infant's health. Donor milk processed in HM banks by Holder pasteurization (HoP; 62.5°C, 30 min) is a safe and valuable resource for preterm newborns that are hospitalized, but is reduced in major immunological components due to thermal inactivation. We hypothesized that high hydrostatic pressure (HHP) and high-pressure homogenization (HPH) are 2 processes that can be used on HM to reduce total bacteria counts while retaining immunological components. We studied the effects of HHP (400, 450, and 500 MPa for 5 min applied at 20°C) and HPH (200, 250, and 300 MPa, milk inlet temperature of 20°C) applied to mature HM, on microbiological and immunological markers (IgA, IgG, IgM, sCD14, and TGF-β2), and compared them with those of traditional HoP in HM samples from healthy donors. The HHP processing between 400 and 500 MPa at 20°C reduced counts of coliform and total aerobic bacteria to undetectable levels (<1.0 log cfu/mL) while achieving approximately 100% of immunological component retention. In particular, comparing median percentages of retention of immunological components for 450 MPa versus HoP, we found 101.5 versus 50.5% for IgA, 89.5 versus 26.0% for IgM, 104.5 versus 75.5% for IgG, 125.0 versus 72.5% for lysozyme, 50.6 versus 0.1% for sCD14, and 88.5 versus 61.1% for TGF-β2, respectively. Regarding HPH processing, at a pressure of 250 MPa and inlet temperature of 20°C, the process showed good potential to reduce coliforms to undetectable levels and total aerobic bacteria to levels slightly above those obtained by HoP. The median percentages of retention of immunological markers for HPH versus HoP were 71.5 versus 52.0%, 71.0 versus 27.0%, 104.0 versus 66.5%, and 30.9 versus 0.2%, for IgA, IgM, IgG, and sCD14, respectively; results did not significantly differ for lysozyme and TGF-β2. The HPH at 300 MPa produced higher inactivation of immunological components, similar to values achieved with HoP.

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The human milk microbiome and associated factors

Neu

2021

The Microbiome in Prenatal and Neonatal Life

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Impact of Holder pasteurization on immunological properties of human breast milk over the first year of lactation

Cited by 18 publications

References 36 publications

Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

High-pressure homogenization and high hydrostatic pressure processing of human milk: Preservation of immunological components for human milk banks

The human milk microbiome and associated factors

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