Impact of Heterogeneity within Cultured Cells on Bacterial Invasion: Analysis of
            <i>Pseudomonas aeruginosa</i>
            and
            <i>Salmonella enterica</i>
            Serovar Typhi Entry into MDCK cells by Using a Green Fluorescent Protein-Labelled Cystic Fibrosis Transmembrane Conductance Regulator Receptor

Gerçeker, A. Alev; Zaidi, Tanweer; Marks, Peter; Golan, David E.; Pier, Gerald B.

doi:10.1128/iai.68.2.861-870.2000

Cited by 26 publications

(23 citation statements)

References 21 publications

(24 reference statements)

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“…It should be noted that Fig. 6B through E cannot be interpreted quantitatively, since this cell line demonstrates high cell-to-cell variability in expression of the CFTR-GFP fusion protein (8). These data can only be analyzed qualitatively as an indication of CFTR-GFP localization within the cells.…”

Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Typhi Modulates Cell Surface Expression of Its Receptor, the Cystic Fibrosis Transmembrane Conductance Regulator, on the Intestinal Epithelium

Lyczak

Pier

2002

Infect Immun

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The cystic fibrosis transmembrane conductance regulator (CFTR) protein is an epithelial receptor mediating the translocation of Salmonella enterica serovar Typhi to the gastric submucosa. Since the level of cell surface CFTR is directly related to the efficiency of serovar Typhi translocation, the goal of this study was to measure CFTR expression by the intestinal epithelium during infection. CFTR protein initially present in the epithelial cell cytoplasm was rapidly trafficked to the plasma membrane following exposure to live serovar Typhi or bacterial extracts. CFTR-dependent bacterial uptake by epithelial cells increased (>100-fold) following CFTR redistribution. The bacterial factor which triggers CFTR redistribution is heat and protease sensitive. These data suggest that serovar Typhi induces intestinal epithelial cells to increase membrane CFTR levels, leading to enhanced bacterial ingestion and submucosal translocation. This could be a key, early step in the infectious process leading to typhoid fever.Salmonella enterica serovar Typhi causes typhoid or enteric fever and initiates infection by entering the enterocytes and specialized M cells of the small intestine, after which the bacterium is translocated to the intestinal submucosa and ingested by host macrophages. The mechanism by which serovar Typhi enters enterocytes is complex and involves intercellular adhesins (5) and intracellular signaling components (18) which modify the host cell cytoskeletal (6) and vacuolar (7) organization. This process is initiated when adhesins and signaling components are delivered into the host cell by the bacterium via the bacterial type III secretion apparatus (4). Since assembly of the type III secretion apparatus is stimulated by bacterium-epithelial cell contact (9), early interactions between serovar Typhi and the epithelial cell play an important role in the initiation of infection.

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Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Typhi Modulates Cell Surface Expression of Its Receptor, the Cystic Fibrosis Transmembrane Conductance Regulator, on the Intestinal Epithelium

Lyczak

Pier

2002

Infect Immun

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“…In the results shown in Fig. 5, internalized bacteria were detected in scans 15 (7.5 m from the apical surface) to 19 (9.5 m from the apical surface) from a total of 23 scans; scans 14,17,19, and 21 are shown. The main location for uptake of bacteria into epithelial cells appeared to be on the edge of the transition of ciliated to nonciliated, possibly denuded, areas.…”

Section: Visualization Of P Aeruginosa Attachment and Internalizatiomentioning

confidence: 94%

“…Our previous work (1)(2)(3)17) has shown that CFTR is an epithelial cell receptor for uptake of P. aeruginosa. We have proposed that this process is critical to removal of P. aeruginosa from the respiratory tract.…”

Section: Discussionmentioning

confidence: 99%

Transgenic Cystic Fibrosis Mice Exhibit Reduced Early Clearance of Pseudomonas aeruginosa from the Respiratory Tract

Schroeder

Reiniger

Meluleni

et al. 2001

The Journal of Immunology

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The cystic fibrosis (CF) transmembrane conductance regulator (CFTR) has been proposed to be an epithelial cell receptor for Pseudomonas aeruginosa involved in bacterial internalization and clearance from the lung. We evaluated the role of CFTR in clearing P. aeruginosa from the respiratory tract using transgenic CF mice that carried either the ΔF508 Cftr allele or an allele with a Cftr stop codon (S489X). Intranasal application achieved P. aeruginosa lung infection in inbred C57BL/6 ΔF508 Cftr mice, whereas ΔF508 Cftr and S489X Cftr outbred mice required tracheal application of the inoculum to establish lung infection. CF mice showed significantly less ingestion of LPS-smooth P. aeruginosa by lung cells and significantly greater bacterial lung burdens 4.5 h postinfection than C57BL/6 wild-type mice. Microscopy of infected mouse and rhesus monkey tracheas clearly demonstrated ingestion of P. aeruginosa by epithelial cells in wild-type animals, mostly around injured areas of the epithelium. Desquamating cells loaded with P. aeruginosa could also be seen in these tissues. No difference was found between CF and wild-type mice challenged with an LPS-rough mucoid isolate of P. aeruginosa lacking the CFTR ligand. Thus, transgenic CF mice exhibit decreased clearance of P. aeruginosa and increased bacterial burdens in the lung, substantiating a key role for CFTR-mediated bacterial ingestion in lung clearance of P. aeruginosa.

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“…Short circuit current measurements showed that the protein mediated cAMP-activated transepithelial chloride transport across monolayers of stably transfected MDCK cells (Moyer et al, 1998). Studies of the dynamics of CFTR protein responses to bacterial infections, the manner by which the CFTR protein responds to, interacts with, and mediates translocation of P. aeruginosa and serovar S. typhi from the cell surface into the cell were also done using a GFP-CFTR fused protein (Gerçeker et al, 2000). …”

Section: Construction and In Vitro Applications Of Gfp-cftrmentioning

confidence: 99%

CFTR Gene Transfer and Tracking the CFTR Protein in the Airway Epithelium

Gonzalez¹,

Boulanger²,

Hong³

2012

Cystic Fibrosis - Renewed Hopes Through Research

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Impact of Heterogeneity within Cultured Cells on Bacterial Invasion: Analysis of Pseudomonas aeruginosa and Salmonella enterica Serovar Typhi Entry into MDCK cells by Using a Green Fluorescent Protein-Labelled Cystic Fibrosis Transmembrane Conductance Regulator Receptor

Cited by 26 publications

References 21 publications

Salmonella enterica Serovar Typhi Modulates Cell Surface Expression of Its Receptor, the Cystic Fibrosis Transmembrane Conductance Regulator, on the Intestinal Epithelium

Salmonella enterica Serovar Typhi Modulates Cell Surface Expression of Its Receptor, the Cystic Fibrosis Transmembrane Conductance Regulator, on the Intestinal Epithelium

Transgenic Cystic Fibrosis Mice Exhibit Reduced Early Clearance of Pseudomonas aeruginosa from the Respiratory Tract

CFTR Gene Transfer and Tracking the CFTR Protein in the Airway Epithelium

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