Impact of heavy metals pollution on molecular genetics of some medicinal plants

Abstract

Heavy metals are natural constituents of the environment, but indiscriminate use for human purposes has altered their biochemical and molecular genetic balance. Prolonged exposure and higher accumulation of such heavy metals can have deleterious health effects on human life. Impact of heavy metals pollution may be effect on plant in the DNA molecular genetics level. In the present investigation we focus to evaluate the pollution of heavy metals among three plant species from two sites of polluted and non polluted regions based on analysis of molecular genetics level of ISSR, AFLP. Five out of the 10 ISSR primers were HB9, HB10, HB11, HB12 and HB14 which were succeed to amplify 172 reproducible and polymorphic bands on the other hand AFLP analysis also was used depend on pairs of primers EcoR I-ACA and MseI-CTC which provided a total of 116 bands ranging from 1550 to 154 bp. Molecular genetics ISSR and AFLP markers appeared more significant differences between polluted and non polluted plants which will provide a new insight for better understanding of the molecular basis of nutritional stress responses of wild medicinal plants to pollution which reflect the genetic defense action and reaction against genetically through appearance some bands product on the transcription and translation level.