Impact of growth regulators on callus formation and regeneration of ornamental plant zamiifolia

Abstract

Aim: This study aimed to access the rapid and improved propagation protocol production of Zamifolia plant using tissue culture. Material and Methods: For callus formation and regeneration, two independent factorial experiments were carried out in a completely randomized design with 3 replications in the tissue culture laboratory of the National Research Institute of Flowers and Ornamental Plants in 2018. In the first experiment, to induce callogenesis, 2,4-D (0, 1, 2, and 4 mg/l) and BA (0, 1 and 2 mg/l) were used. In the second experiment, or regeneration of explants, NAA (0 and 0.5 mg/l) and BA (0, 0.5, and 1 mg/l) were applied. Results:The results of the callogenesis experiment showed that a modified basal MS medium with concentration of 2 mg/l BA and 1 mg /l 2,4-D produced the best callus with 85% regeneration. At the regeneration stage of the callus, 0.5 mg/l BA and 0.5 mg NAA produced the highest number of seedlings and regenerated tubers (90%). Subsequently, single tuber seedlings were successfully adapted to 80%-90% in the cocopeat and perlite substrates. Conclusion:The results were successful and could be possible to achieve the callogenesis and regeneration of Zamifolia plant with the formula presented and recommend as practical a protocol for commercial micropropagation of this valuable ornamental plant.

Evaluating the effect of gamma rays on Zamiifolia (Zamioculcas zamiifolia) plant in vitro and genetic diversity of the resulting genotypes using the ISSR marker

“…Then washing was performed in three steps (5, 10, and 15 min) under the laminar machine. The sterilized leaves parts were divided under the laminar into 1 × 1 cm pieces containing the main vein and cultivated in jars containing the culture medium 16 .…”

“…The basic culture mediums of this experiment to induce callus were MS, 2,4-D growth regulators at three levels (0, 1 and 2 mg/l) and BA at three levels (0, 1, and 2 mg/l) 16 .…”

“…After the emergence of callus and reaching the appropriate volume in terms of size and quality, all calluses were transferred to a new culture medium containing MS base culture medium for regeneration and plant production and then transferred together with NAA (0, 0.5 and 1 mg/l) and BA (0, 0.5 and 1 mg/l). The sucrose used was 30 g/l 1iter, and the pH of the medium was set to 5.7 16 . A factorial experiment based on a completely random design was used to determine the best treatments and their interaction effects.…”