Impact of growth conditions on transport behavior of E. coli

Marcus, Ian M.; Bolster, Carl H.; Cook, Kyle DeMeo; Opot, Stephen R.; Walker, Sharon L.

doi:10.1039/c2em10960c

Cited by 15 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in previous work using regression analysis, attachment efficiency was found to be correlated to electrophoretic mobility (72)(73)(74) and hydrophobicity (77). Additionally, attachment efficiency was inversely proportional to the EPS sugar-to-protein ratio (P Ͻ 0.05) (74).…”

Section: Discussionsupporting

confidence: 48%

“…The extensive phenotypic cell characteristics tested-hydrophobicity, electrophoretic mobility (EPM), radius, extracellular polymeric substance (EPS) content, and attachment efficiencywere chosen because they have been linked to the fate and transport of microorganisms in the environment (42,(72)(73)(74) and can be used for comparative evaluation of different environmental microbial communities. In the absence of E. coli O157:H7, cells from the colon had lower hydrophobicity, EPM, EPS, and attachment efficiency values than microbial communities in the aquatic systems.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Linking Microbial Community Structure to Function in Representative Simulated Systems

Marcus

Wilder

Quazi

et al. 2013

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Pathogenic bacteria are generally studied as a single strain under ideal growing conditions, although these conditions are not the norm in the environments in which pathogens typically proliferate. In this investigation, a representative microbial community along with Escherichia coli O157:H7, a model pathogen, was studied in three environments in which such a pathogen could be found: a human colon, a septic tank, and groundwater. Each of these systems was built in the lab in order to retain the physical/ chemical and microbial complexity of the environments while maintaining control of the feed into the models. The microbial community in the colon was found to have a high percentage of bacteriodetes and firmicutes, while the septic tank and groundwater systems were composed mostly of proteobacteria. The introduction of E. coli O157:H7 into the simulated systems elicited a shift in the structures and phenotypic cell characteristics of the microbial communities. The fate and transport of the microbial community with E. coli O157:H7 were found to be significantly different from those of E. coli O157:H7 studied as a single isolate, suggesting that the behavior of the organism in the environment was different from that previously conceived. The findings in this study clearly suggest that to gain insight into the fate of pathogens, cells should be grown and analyzed under conditions simulating those of the environment in which the pathogens are present.

show abstract

Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Linking Microbial Community Structure to Function in Representative Simulated Systems

Marcus

Wilder

Quazi

et al. 2013

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Within the current body of literature, little attention has been paid to the fate of waterborne pathogenic microbes under nutrient-deficient conditions that prevail in the natural environment and wastewater treatment facilities (17,(22)(23)(24). Walker reported that, for both Burkholderia cepacia G4g and ENV435g, the deposition rates for cells cultivated in nutrient-rich medium differed from those in nutrient-poor basal salt medium (17).…”

mentioning

confidence: 99%

“…Walker reported that, for both Burkholderia cepacia G4g and ENV435g, the deposition rates for cells cultivated in nutrient-rich medium differed from those in nutrient-poor basal salt medium (17). In addition, Marcus et al investigated the effect of the growth solution on the cell surface properties and transport behavior of 11 Escherichia coli isolates, and they observed significant differences in cell surface properties and transport behavior for some cells according to the growth solution (24). However, to our knowledge, no such studies have been performed on bacterial deposition and transport in aqueous chemistry relevant to treated wastewater.…”

mentioning

confidence: 99%

Retention in Treated Wastewater Affects Survival and Deposition of Staphylococcus aureus and Escherichia coli in Sand Columns

Zhao

Tian

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

The fate and transport of pathogenic bacteria from wastewater treatment facilities in the Earth's subsurface have attracted extensive concern over recent decades, while the impact of treated-wastewater chemistry on bacterial viability and transport behavior remains unclear. The influence of retention time in effluent from a full-scale municipal wastewater treatment plant on the survival and deposition of Staphylococcus aureus and Escherichia coli strains in sand columns was investigated in this paper. In comparison to the bacteria cultivated in nutrient-rich growth media, retention in treated wastewater significantly reduced the viability of all strains. Bacterial surface properties, e.g., zeta potential, hydrophobicity, and surface charges, varied dramatically in treated wastewater, though no universal trend was found for different strains. Retention in treated wastewater effluent resulted in changes in bacterial deposition in sand columns. Longer retention periods in treated wastewater decreased bacterial deposition rates for the strains evaluated and elevated the transport potential in sand columns. We suggest that the wastewater quality should be taken into account in estimating the fate of pathogenic bacteria discharged from wastewater treatment facilities and the risks they pose in the aquatic environment.

show abstract

“…The impact on various growth conditions on the transport of E. coli was also investigated (Marcus et al, 2012). When measuring E. coli grown in a standard laboratory growth medium and a dairy manure extract solution, cells grown in manure extract were more hydrophobic, had a more negative zeta potential, had lower amounts of surface macromolecules, and had lower attachment efficiencies than cells grown in laboratory growth medium.…”

Section: Occurrence Of Fecal Indicators In Watershedsmentioning

confidence: 99%

Detection and Occurrence of Indicator Organisms and Pathogens

Kronlein¹,

Stedtfeld²,

Sorensen³

et al. 2013

Water Environment Research

View full text Add to dashboard Cite

This review summarizes the literature published in 2012 pertaining to the occurrence and detection of fecal indicator bacteria (FIB) and pathogens. It is organized into the following sections: i) methods for the detection and quantification of waterborne fecal indicators and pathogens including polymerase chain reaction (PCR), isothermal amplification, label-free methods, microarrays, immunoassay-based methods, microfluidic-based methods, and advances in sample processing and concentration at the limit of detection (LOD), ii) microbial source tracking (MST) using both genotypic and phenotypic-based methods, iii) environmental occurrence, persistence, and transport of fecal indicators and pathogens.

show abstract

Impact of growth conditions on transport behavior of E. coli

Cited by 15 publications

References 50 publications

Linking Microbial Community Structure to Function in Representative Simulated Systems

Linking Microbial Community Structure to Function in Representative Simulated Systems

Retention in Treated Wastewater Affects Survival and Deposition of Staphylococcus aureus and Escherichia coli in Sand Columns

Detection and Occurrence of Indicator Organisms and Pathogens

Contact Info

Product

Resources

About