Impact of groundwater samples and leachates from Gbagede dumpsite, Amoyo, Kwara State, Nigeria, on testes and prostate of male Wistar rats: A biochemical and histological study

Abstract

This study evaluated the impact of groundwater samples and leachate from Gbagede dumpsite in Amoyo, Kwara State, on the testicular and prostatic function indices of male rats. The groundwater sample 1 (GW1), groundwater sample 2 (GW2), 2.5%, 5.0%, 7.5% and 10.0% leachate progressively reduced (p < .05) feed intake, groundwater and leachate intake, body weight, weights of testes and prostate, and testes–body weight ratio. The groundwater and leachates significantly (p < .05) reduced the sperm count, motility, normal morphology and testicular volume; activities of semen acid phosphatase (ACP) and α‐glucosidase, testicular alkaline phosphatase, ACP, ᵧ‐GT, lactate dehydrogenase, glutathione reductase, catalase and superoxide dismutase; testicular total protein, glycogen, total cholesterol, sialic acid, testosterone, reduced glutathione, total antioxidant capacity, zinc, iron and copper; serum LH and FSH; prostatic calcium and phosphate. Treatments increased testicular malondialdehyde, prostate‐specific antigen and ACP whilst prostatic pH remained significantly unaltered. Only the leachates reduced prostate–body weight ratio. The treatments induced distortions of seminiferous tubules, destroyed spermatogonic cells and degenerated prostatic acinus. The study concluded that metals in the groundwater and leachate samples have adversely impacted on the testes and prostates of the male rats via endocrine disruption and oxidative stress, with attendant implications on reproductive capacity.

Kandungan Logam Berat dan Vitamin E pada Testis Tikus Wistar setelah Dipaparkan Air Lindi

“…Penyebab abnormalitas tersebut adalah komponen yang ada di dalam air lindi. Salah satu senyawa yang dapat menyebabkan gangguan reproduksi yang ada di air lindi adalah logam berat (Yakubu & Omar, 2020). Logam berat dapat merusak struktur testis, gangguan hormonal dan gangguan spermatogenesis sehingga menghasilkan spermatozoa abnormal (He et al, 2020).…”

“…Penurunan konsentrasi vitamin E testis pada perlakuan air lindi 63% sebesar 86,6 % jika dibandingkan dengan perlakuan akuabides. Paparan air lindi menyebabkan ketidakseimbangan pro-oksidan/antioksidan sehingga meningkatkan radikal pada testis (Yakubu & Omar, 2020). Peningkatan ROS seperti lipid peroksida dan gangguan level antioksidan menyebabkan penurunan kualitas sperma dan disfungsi tesis (Asadi et al, 2017).…”