Impact of grassland management regimes on bacterial endophyte diversity differs with grass species

Wemheuer, Franziska; Wemheuer, Bernd; Kretzschmar, Daniel; Pfeiffer, Birgit; Herzog, Sarah; Daniel, Rolf; Vidal, Stefan

doi:10.1111/lam.12551

Cited by 29 publications

(37 citation statements)

References 30 publications

(69 reference statements)

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“…Recent research has shown that bacterial endophytic communities in plants within the Poaceae family are affected by fertilizer application789. In two previous studies, fertilizer application influenced the nitrogen-fixing (diazotrophic) bacterial community in maize roots and stems8 as well as in rice roots10.…”

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confidence: 99%

Bacterial endophyte communities of three agricultural important grass species differ in their response towards management regimes

Wemheuer

Kaiser

Karlovský

et al. 2017

Sci Rep

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Endophytic bacteria are critical for plant growth and health. However, compositional and functional responses of bacterial endophyte communities towards agricultural practices are still poorly understood. Hence, we analyzed the influence of fertilizer application and mowing frequency on bacterial endophytes in three agriculturally important grass species. For this purpose, we examined bacterial endophytic communities in aerial plant parts of Dactylis glomerata L., Festuca rubra L., and Lolium perenne L. by pyrotag sequencing of bacterial 16S rRNA genes over two consecutive years. Although management regimes influenced endophyte communities, observed responses were grass species-specific. This might be attributed to several bacteria specifically associated with a single grass species. We further predicted functional profiles from obtained 16S rRNA data. These profiles revealed that predicted abundances of genes involved in plant growth promotion or nitrogen metabolism differed between grass species and between management regimes. Moreover, structural and functional community patterns showed no correlation to each other indicating that plant species-specific selection of endophytes is driven by functional rather than phylogenetic traits. The unique combination of 16S rRNA data and functional profiles provided a holistic picture of compositional and functional responses of bacterial endophytes in agricultural relevant grass species towards management practices.

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confidence: 99%

Bacterial endophyte communities of three agricultural important grass species differ in their response towards management regimes

Wemheuer

Kaiser

Karlovský

et al. 2017

Sci Rep

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“…The diazotrophic community can be altered, especially by soil characteristics, by exudation patterns of the plants [32], by type of vegetation, among plant genotypes, by phenological stage, and by niche of colonization [6,10,26,33], besides the action of animals on the vegetation, during grazing [34]. However, the association of these bacteria with forage grasses constitutes an important resource to be used in the recovery of areas with soil and grasses in various stages of degradation [7,30].…”

Section: Cultivated (Not Validated) Diazotrophic Bacterial Communitymentioning

confidence: 99%

“…Therefore, due to the capacity to colonize intra-and intercellular spaces, the endophytic microorganisms have a close relationship with their host [10], which contributed to reducing their diversity. The advance of the knowledge about the microbial diversity was provided by the evolution of the methodologies of molecular biology applied to the study of the environment [37].…”

Section: Degradation Stage and Association Regionmentioning

confidence: 99%

“…Impacts on the community of microorganisms associated with the plant and present in the soil are expected after the use of chemical fertilizers [28,29] and changes in the management [10].…”

Section: Cultivated (Not Validated) Diazotrophic Bacterial Communitymentioning

confidence: 99%

“…Although the importance of these bacteria in cultivation systems of forage grasses is frequently acknowledged [1], the interactions between these 2 Applied and Environmental Soil Science microorganisms and these plants under different management regimes are not yet well understood [10]. In spite of that, the comparative study of the functional beta-diversity of bacterial communities in contrasting environments has been used as a parameter to indicate stability of biotic and abiotic phenomena in ecosystems [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Diazotrophic Bacterial Community of Degraded Pastures

Oliveira

Figueredo

Diniz

et al. 2017

Applied and Environmental Soil Science

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Pasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation. Samples of roots and rhizospheric soil were collected from slightly, partially, and highly degraded pastures. McCrady's table was used to obtain the Most Probable Number (MPN) of bacteria per gram of sample, in order to determine population density and calculate the Shannon-Weaver diversity index. The diversity of total diazotrophic bacterial community was determined by the technique of Denaturing Gradient Gel Electrophoresis (DGGE) of the nif H gene, while the diversity of the culturable diazotrophic bacteria was determined by the Polymerase Chain Reaction (BOX-PCR) technique. The increase in the degradation stage of the B. decumbens Stapf. pasture did not reduce the population density of the cultivated diazotrophic bacterial community, suggesting that the degradation at any degree of severity was highly harmful to the bacteria. The structure of the total diazotrophic bacterial community associated with B. decumbens Stapf. was altered by the pasture degradation stage, suggesting a high adaptive capacity of the bacteria to altered environments.

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Assessing Bacterial and Fungal Diversity in the Plant Endosphere

Wemheuer

2016

Methods in Molecular Biology

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Plants are colonized various microorganisms including endophytes. These microbes can play an important role in agricultural production as they promote plant growth and/or enhance the resistance of their host plant against diseases and environmental stress conditions. Although culture-independent molecular approaches such as DNA barcoding have greatly enhanced our understanding of bacterial and fungal endophyte communities, there are some methodical problems when investigating endophyte diversity. One main issue are sequence contaminations such as plastid-derived rRNA gene sequences which are co-amplified due to their high homology to bacterial 16S rRNA genes. The same is true for plant and fungal ITS sequences. The application of highly specific-primers suppressing co-amplification of these sequence contaminations is a good solution for this issue. Here, we describe a detailed protocol for assessing bacterial and fungal endophyte diversity in plants using these primers in combination with next-generation sequencing.

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Impact of grassland management regimes on bacterial endophyte diversity differs with grass species

Cited by 29 publications

References 30 publications

Bacterial endophyte communities of three agricultural important grass species differ in their response towards management regimes

Bacterial endophyte communities of three agricultural important grass species differ in their response towards management regimes

Diazotrophic Bacterial Community of Degraded Pastures

Assessing Bacterial and Fungal Diversity in the Plant Endosphere

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