Impact of glutathione-HbA1c on HbA1c measurement in diabetes diagnosis via array isoelectric focusing, liquid chromatography, mass spectrometry and ELISA

Li, Si; Guo, Chaowan; Lü, Chen; Yin, Xiao-Yang; Wu, Yixin; Fan, Liu-Yin; Fan, Hongsong; Cao, Cheng-Xi

doi:10.1016/j.talanta.2013.05.040

Cited by 7 publications

(2 citation statements)

References 30 publications

(22 reference statements)

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“…Furthermore precise quantification of HbA1c using available methods in routine diagnostics, such as ion exchange [ 7 ], phenylboronate affinity [ 8 ] or antibody based chromatography [ 9 ], are technically challenging. For example, ion exchange based measurement of HbA1c is influenced by other chemical modifications such as glutathionylation [ 10 ]. Whereas boronate affinity chromatography invariably detects the cis -diol groups of glycated hemoglobin not limited to DFV but also includes other amino acids like lysine and arginine, which may result in overestimation of HbA1c [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Targeted quantification of N-1-(carboxymethyl) valine and N-1-(carboxyethyl) valine peptides of β-hemoglobin for better diagnostics in diabetes

Jagadeeshaprasad

Batkulwar

Meshram³

et al. 2016

Clin Proteom

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BackgroundN-1-(Deoxyfructosyl) valine (DFV) β-hemoglobin (β-Hb), commonly referred as HbA1c, is widely used diagnostic marker in diabetes, believed to provide glycemic status of preceding 90–120 days. However, the turnover of hemoglobin is about 120 days, the DFV-β-Hb, an early and reversible glycation product eventually may undergo irreversible advanced glycation modifications such as carboxymethylation or carboxyethylation. Hence quantification of N-1-(carboxymethyl) valine (CMV) and N-1-(carboxyethyl) valine (CEV) peptides of β-Hb would be useful in assessing actual glycemic status.ResultsFragment ion library for synthetically glycated peptides of hemoglobin was generated by using high resolution–accurate mass spectrometry (HR/AM). Using parallel reaction monitoring, deoxyfructosylated, carboxymethylated and carboxyethylated peptides of hemoglobin were quantified in clinical samples from healthy control, pre-diabetes, diabetes and poorly controlled diabetes. For the first time, we report N-1-β-valine undergoes carboxyethylation and mass spectrometric quantification of CMV and CEV peptides of β-hemoglobin. Carboxymethylation was found to be the most abundant modification of N-1-β-valine. Both CMV-β-Hb and CEV-β-Hb peptides showed better correlation with severity of diabetes in terms of fasting glucose, postprandial glucose and microalbuminuria.ConclusionsThis study reports carboxymethylation as a predominant modification of N-1-β-valine of Hb, and quantification of CMV-β-Hb and CEV-β-Hb could be useful parameter for assessing the severity of diabetes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12014-016-9108-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Targeted quantification of N-1-(carboxymethyl) valine and N-1-(carboxyethyl) valine peptides of β-hemoglobin for better diagnostics in diabetes

Jagadeeshaprasad

Batkulwar

Meshram³

et al. 2016

Clin Proteom

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“…For the extraction of pure hemoglobin (Hb), blood was collected from the exsanguinated chicken samples. Hemoglobin sample was obtained from the red blood cells in accordance with the standard protocol with some modifications. Further details are given in the .…”

Section: Methodsmentioning

confidence: 99%

A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China

Saud

Sun

et al. 2019

Electrophoresis

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A novel analytical protocol was developed for general quality screening of chicken meat based on IEF and protein extraction. To demonstrate the developed protocol, 24 chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in water, and that in Group 3 were infected by new castle disease virus. Proteins were extracted from the meat samples by using pure water as an extractant, separated by IEF, verified by western blot, and quantified via imaging analysis. The relevant experiments demonstrated that two myoglobin (Mb) bands were detected at pI 6.8 and 7.04 for all samples of Groups 1, 2, and 3, but there were additional hemoglobin (Hb) bands at pI 7.09 and 7.13 (P < 0.05) for the samples of Groups 2 and 3. The results implied that Hb bands might be a potential biomarker for the screening of chicken meat quality. The RSD values of two Mb bands (pI 6.8 and 7.04) in Group 1 were respectively 4.08 and 3.63%, the ones of two Hb bands (pI 7.09 and 7.13) in Group 2 were 3.66 and 2.10%, and those in Group 3 were 2.17% and 2.77%, respectively. All the RSD values indicated high stability and reliability of the developed protocol. Additionally, the protocol had a direct readout of protein bands in IEF without staining. However, it was time‐consuming and had high cost. Even so, the relevant general method and finding have potential for screening of chicken meat quality.

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Isoelectric focusing array with immobilized pH gradient and dynamic scanning imaging for diabetes diagnosis

Dong

et al. 2019

Analytica Chimica Acta

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Impact of glutathione-HbA1c on HbA1c measurement in diabetes diagnosis via array isoelectric focusing, liquid chromatography, mass spectrometry and ELISA

Cited by 7 publications

References 30 publications

Targeted quantification of N-1-(carboxymethyl) valine and N-1-(carboxyethyl) valine peptides of β-hemoglobin for better diagnostics in diabetes

Targeted quantification of N-1-(carboxymethyl) valine and N-1-(carboxyethyl) valine peptides of β-hemoglobin for better diagnostics in diabetes

A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China

Isoelectric focusing array with immobilized pH gradient and dynamic scanning imaging for diabetes diagnosis

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