Impact of freeze-drying and subsequent storage on milk metabolites based on 1H NMR and UHPLC-QToF/MS

Zhu, Dan; Kebede, Biniam; Chen, Gang; McComb, Kiri; Frew, Russell

doi:10.1016/j.foodcont.2019.107017

Cited by 22 publications

(18 citation statements)

References 44 publications

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“…However, Hamid et al [ 23 ] did not directly compare lyophilisation to liquid nitrogen extraction. Zhu et al [ 27 ] have shown that freeze-drying milk to produce milk powders has a limited effect on the milk metabolites; this study also showed stability in the metabolome when stored at either 4 or −20 °C, but storage at room temperature resulted in significant changes. Cheng et al [ 28 ] suggest using lyophilised faecal samples, rather than fresh frozen samples.…”

Section: Resultssupporting

confidence: 56%

Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs

et al. 2021

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Metabolomics is a technique that allows for the evaluation of the entire extractable chemical profile of a plant, for example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due to drought. Metabolomic analysis is dependent upon the efficiency of the extraction protocol. Currently, there are two common extraction procedures widely used in metabolomic experiments, those that extract from plant tissue processed in liquid nitrogen or extraction from lyophilised plant tissues. Here, we evaluated the two using non-targeted metabolomics to show that lyophilisation can stabilise the maize (Zea mays) extractable metabolome, increasing throughput and efficiency of extraction as compared to the more traditional processing in liquid nitrogen. Then, we applied the lyophilisation approach to explore the effect of drought upon the maize metabolome in a non-targeted HRMS metabolomics approach. Metabolomics revealed differences in the mature maize metabolome having undergone three drought conditions imposed at two critical development stages (three-leaf stage and grain-fill stage); moreover, this difference was observed across two tissue types (kernel and inner cob/pith). It was shown that under ideal conditions, the biochemical make-up of the tissue types is different. However, under stress conditions, the stress response dominates the metabolic profile. Drought-related metabolites known from other plant systems have been identified and metabolomics has revealed potential novel drought-stress indicators in our maize system.

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Section: Resultssupporting

confidence: 56%

Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs

et al. 2021

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“…These results were also confirmed by Zhu et al (2020a). The presence of N-acetylglucosamine and N-acetylgalactosamine in bovine milk in connection to freeze-drying and subsequent storage was also described by Zhu et al (2020b). The N-acetyl carbohydrates in caprine milk have also been studied in relation to the milk metabolome and the anti-adhesive effect of these compounds on bacterial, viral, and protozoan pathogens, preventing them from binding to mammary tissue.…”

Section: Role Of N-acetyl Carbohydrates In Bovine and Caprine Milkmentioning

confidence: 65%

Detection of bovine milk adulteration in caprine milk with N-acetyl carbohydrate biomarkers by using 1H nuclear magnetic resonance spectroscopy

Rysová

Legarová

Pacáková

et al. 2021

Journal of Dairy Science

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In a return to tradition, the popularity of caprine milk is on the rise. However, particularly in countries with developed dairy industries based on bovine milk, there is the risk of adulteration with bovine milk, which is a cheaper alternative. Thus, a rapid, robust, and simple method for the detection of bovine milk added to caprine milk is necessary, and 1 H nuclear magnetic resonance spectroscopy appears to provide a solution. A matrix of 115 pure and artificially adulterated pasteurized milk samples was prepared and used to discover biomarkers of bovine milk that are independent of chemical and biological variation caused by factors such as genetics, diet, or seasonality. Principal component analysis and orthogonal projections to latent structures discriminant analysis of pure bovine milk and pure caprine milk revealed spectral features that were assigned to the resonances of 4 molecules. Of these, the peaks corresponding to protons in the N-acetylglucosamine and N-acetylgalactosamine acetyl moieties showed significant applicability for our method. Receiver operating characteristic curve analysis was used to evaluate the performance of the peak integrals as biomarkers of adulteration. This approach was able to distinguish caprine milk adulterated with 5% of bovine milk with 84.78% accuracy and with 10% of bovine milk an excellent 95.65% accuracy. This study demonstrates that N-acetyl carbohydrates could be used as biomarkers for the detection of bovine milk in caprine milk and could help in protecting caprine milk authenticity.

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“…The stability of the metabolome of milk powders underwent minor changes after freeze-drying as well as after storage at 4°C and − 20°C, while significant changes occurred in samples stored at 20°C. The study has revealed the importance of the prior storage temperature of milk when applying metabolomics measurements in the verification of the origin or of the authenticity of milk samples because the changes in metabolites sensitive to storage are very important (Zhu et al, 2020b).…”

Section: Nmr Spectroscopymentioning

confidence: 99%

Application of Spectroscopic Techniques to Evaluate Heat Treatments in Milk and Dairy Products: an Overview of the Last Decade

Aït‐Kaddour

Hassoun

Bord

et al. 2021

Food Bioprocess Technol

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In the food industry, thermal treatments are generally an essential step to increase the shelf life of the products. This is especially true for milk and dairy products in which heat treatments help to eliminate pathogenic organisms, minimize microbiological development, and improve some sensory properties. However, they can also induce biochemical, physico-chemical, and sensory changes in foods, and then adversely affect the final quality of the products. To assess the quality of milk and dairy products during heating, some nondestructive techniques exist. In this article, the application of spectroscopic non-destructive techniques (fluorescence, infrared, NMR) is analyzed to point out the pertinence by using them as tools to monitor milk and dairy product quality changes during heating. An overview of the last studies on the effect of different conventional and emerging methods of milk and dairy product heating on biochemical, physico-chemical, and sensory quality is also presented, as well as the perspectives of research in this topic.

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Impact of freeze-drying and subsequent storage on milk metabolites based on 1H NMR and UHPLC-QToF/MS

Cited by 22 publications

References 44 publications

Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs

Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs

Detection of bovine milk adulteration in caprine milk with N-acetyl carbohydrate biomarkers by using 1H nuclear magnetic resonance spectroscopy

Application of Spectroscopic Techniques to Evaluate Heat Treatments in Milk and Dairy Products: an Overview of the Last Decade

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