Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape

Müller, Henry; Berg, Gabriele

doi:10.1007/s10526-007-9111-3

Cited by 84 publications

(62 citation statements)

References 31 publications

(27 reference statements)

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“…Bfreedom^(Seminis, Holland) seeds were surface-sterilized for 5 min using 2 % NaOCl. Bio-priming was performed in accordance with the modified protocol of Müller and Berg (2008). Seeds were immersed in the cell suspension for 4 h at 20°C under agitation.…”

Section: Seed Treatment (Bio-priming)mentioning

confidence: 99%

Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions

et al. 2015

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Aims Verticillium wilt is difficult to suppress, and causes severe yield losses in a broad range of crops. Five Serratia and five Paenibacillus endophytic isolates showing antagonistic properties against fungal pathogens were compared for their plant growth-promoting (PGP) potential under different plant growth conditions with the objective of evaluating the PGP of endophytic strains in different ad planta systems. Methods Preselected isolates were applied to the surfacesterilized seeds of oilseed rape and cauliflower using biopriming. The isolates' PGP effect and root colonization capacities were compared under gnotobiotic conditions. One strain from each genus was selected and tested for its PGP qualities in sterile and non-sterile soil. Results Serratia treatment resulted in different levels of PGP, while Paenibacillus strains damaged roots under gnotobiotic conditions. P. polymyxa Sb3-1 did not have a significant effect on plant growth in non-sterile soil; however it did promote plant growth in the sterile soil. S. plymuthica 3RP8 and P. polymyxa Sb3-1 were selected for further testing of their biocontrol effect under field conditions. Conclusions The choice of growth environments in the investigation of plant-bacterium interaction is crucial. Non-sterile soil is suggested as the ideal medium for use in studying the PGP effect.

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Section: Seed Treatment (Bio-priming)mentioning

confidence: 99%

Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions

et al. 2015

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“…Seeds were soaked in a bacterial suspension to allow imbibition of water and bacteria into the seed. Bio-priming seeds provided higher control against V. longisporum than coating bacteria on the seed surface (Müller and Berg 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Preliminary investigations in laboratory and greenhouse trials revealed that both bacteria have potential to control Verticillium longisporum and L.maculans in the OSR cultivar Talent and in a field trail a significant reduction of L. maculans leaf infection was recorded after seed bio-priming with S. plymuthica (Hammoudi 2007). As S. plymuthica cells survive less well on the outer surface of OSR seeds (Müller and Berg 2008), the authors applied the bacteria by bio-priming. Seeds were soaked in a bacterial suspension to allow imbibition of water and bacteria into the seed.…”

Section: Introductionmentioning

confidence: 99%

Effect of seed priming with Serratia plymuthica and Pseudomonas chlororaphis to control Leptosphaeria maculans in different oilseed rape cultivars

2011

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The efficacy of a seed treatment of oilseed rape (OSR) (Brassica napus) with the rhizobacteria Serratia plymuthica (strain HRO-C48) and Pseudomonas chlororaphis (strain MA 342) applied alone or in combination against the blackleg disease caused by Leptosphaeria maculans was tested with different cultivars. Seeds were soaked in bacterial suspensions (bio-priming) to obtain log 10 6-7 CFU seed −1 . Cotyledons were inoculated with a 10 ul droplet of L. maculans spore suspension of log 10 7 spores ml −1 and the disease index (size of lesions) was evaluated 14 days later. A mean disease reduction of 71.6% was recorded for S. plymuthica and of 54% for P. chlororaphis. The combined treatment was not superior to the treatment with S. plymuthica alone. The reduction of the disease caused by S. plymuthica was independent of the cultivar's susceptibility, whereas the control effect recorded with P. chlororaphis increased with decreasing cultivar resistance to blackleg disease. The bacterial colonization of OSR was restricted to the roots and hypocotyl. No significant difference in bacterial colonization of the rhizosphere was observed between different cultivars, nor between single or combined bacterial seed treatments.

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“…Potting soil (Einheitserdewerk, Uetersen, Germany) was then mixed with vermiculite and the vermiculitefungal culture at a ratio of 20:5:1 (v/v/v). In healthy controls pots were filled with potting soil mixed with vermiculite (4:1, v/v) (Müller and Berg 2008).…”

Section: Verticillium Longisporum Inoculum Preparation and Soil Infesmentioning

confidence: 99%

“…Both antagonistic bacteria had recently been tested in several OSR cultivars to reduce infection with P. lingam (Abuamsha et al unpublished). Müller and Berg (2008) improved seed treatment techniques for control of V. longisporum with S. plymuthica in greenhouse trials and reported highest control when applying S. plymuthica by bio-priming to OSR seeds. The present study included P. chlororaphis because the existing registration as plant protection product would enable a quicker introduction into practice for this strain than for S. plymuthica, which is not yet registered.…”

Section: Introductionmentioning

confidence: 99%

Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis

2010

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Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis Abstract The effect of a seed treatment with the antagonistic bacteria Serratia plymuthica (strain HRO-C48) and/or Pseudomonas chlororaphis (strain MA 342) on the infection of oilseed rape with Verticillium longisporum was assessed with ten different cultivars. Soil was inoculated with microsclerotia and mycelium of a V. longisporum culture. Seeds were treated with rifampicin-resistant antagonistic bacteria at a rate of log 10 6-7 cells per seed. Resistance against V. longisporum infection did not differ between cultivars and was generally low. A significant disease reduction recorded as area under disease progress curve (AUDPC) was obtained with both antagonistic rhizobacteria with no significant difference between the treatments. Percent of healthy plants was approximately 70% in all bacterial treatments. Significant differences were observed between the cultivars ranging from 46.5% (cultivar Titan) to 72.6% (Trabant). The combined use of both bacteria could not provide additional control effects. The bacterial density in the rhizosphere was not related to the control effect, but increased by log 10 2 on infection with V. longisporum. Growth promotion effects were also not related to the control effect. At present, neither the application of chemical fungicides nor breeding for resistance against V. longisporum in oilseed rape can provide a solution for this increasingly problematic plant pathogen. The present results now open perspectives to control V. longisporum in oilseed rape by making use of cultivars, which express resistance against this pathogen on interaction with the antagonistic rhizobacteria S. plymuthica or P. chlororaphis.

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Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape

Cited by 84 publications

References 31 publications

Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions

Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions

Effect of seed priming with Serratia plymuthica and Pseudomonas chlororaphis to control Leptosphaeria maculans in different oilseed rape cultivars

Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis

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